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Registros recuperados : 2 | |
2. | | PEREIRA, G. R.; LORENZO, P. L.; CARNEIRO, G. F.; BALL, B. A.; GONÇALVES, P. B. D.; PEGORARO, L. M. C.; BILODEAU-GOESEELS, S.; KASTELIC, J. P.; CASEY, P. J.; LIU, I. K. M. The effect of growth hormone (GH) and insulin-like growth factor-I (IGF-I) on in vitro maturation of equine oocytes. Zygote, Cambridge, p. 1-8, 2011. Biblioteca(s): Embrapa Clima Temperado. |
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Registros recuperados : 2 | |
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Registro Completo
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
09/06/2008 |
Data da última atualização: |
29/06/2018 |
Autoria: |
CARNEIRO, A. A.; GOMES, E. A.; GUIMARÃES, C. T.; FERNANDES, F. T.; CARNEIRO, N. P.; CRUZ, I.; CRUZ, I. |
Afiliação: |
ANDREIA ALMEIDA CARNEIRO, CNPMS; ELIANE APARECIDA GOMES, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; FERNANDO TAVARES FERNANDES, CNPMS; NEWTON PORTILHO CARNEIRO, CNPMS; IVAN CRUZ, CNPMS. |
Título: |
Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 4, p. 513-520, abr. 2008 |
Idioma: |
Inglês |
Notas: |
Título em português: Caracterização molecular e virulência de isolados de Beauveria spp. contra a lagarta-do-cartucho. |
Conteúdo: |
The objective of this work was to evaluate the pathogenicity of 24 Beauveria isolates to Spodoptera frugiperda larvae, and characterize them molecularly through rDNA-ITS sequencing and RAPD markers. Sequencing of rDNA-ITS fragments of 570 bp allowed the identification of isolates as B. bassiana or B. brongniarti by sequence comparison to GenBank. Sixty seven polymorphic RAPD fragments were capable to differentiate 20 among 24 Beauveria isolates, grouping them according to the derived host insect and to pathogenicity against maize fall armyworm larvae. Three RAPD markers were highly associated to the pathogenicity against S. frugiperda, explaining up to 67% of the phenotypic variation. Besides identification and molecular characterization of Beauveria isolates, ITS sequence and RAPD markers proved to be very useful in selecting the isolates potentially effective against S. frugiperda larvae and in monitoring field release of these microorganisms in biocontrol programs. |
Palavras-Chave: |
fungo entomopatogênico; RAPD; rDNA-ITS. |
Thesagro: |
Spodoptera Frugiperda. |
Thesaurus NAL: |
entomopathogenic fungi. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/43272/1/43n04a10.pdf
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Marc: |
LEADER 01865naa a2200265 a 4500 001 1124232 005 2018-06-29 008 2008 bl uuuu u00u1 u #d 100 1 $aCARNEIRO, A. A. 245 $aMolecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm. 260 $c2008 500 $aTítulo em português: Caracterização molecular e virulência de isolados de Beauveria spp. contra a lagarta-do-cartucho. 520 $aThe objective of this work was to evaluate the pathogenicity of 24 Beauveria isolates to Spodoptera frugiperda larvae, and characterize them molecularly through rDNA-ITS sequencing and RAPD markers. Sequencing of rDNA-ITS fragments of 570 bp allowed the identification of isolates as B. bassiana or B. brongniarti by sequence comparison to GenBank. Sixty seven polymorphic RAPD fragments were capable to differentiate 20 among 24 Beauveria isolates, grouping them according to the derived host insect and to pathogenicity against maize fall armyworm larvae. Three RAPD markers were highly associated to the pathogenicity against S. frugiperda, explaining up to 67% of the phenotypic variation. Besides identification and molecular characterization of Beauveria isolates, ITS sequence and RAPD markers proved to be very useful in selecting the isolates potentially effective against S. frugiperda larvae and in monitoring field release of these microorganisms in biocontrol programs. 650 $aentomopathogenic fungi 650 $aSpodoptera Frugiperda 653 $afungo entomopatogênico 653 $aRAPD 653 $arDNA-ITS 700 1 $aGOMES, E. A. 700 1 $aGUIMARÃES, C. T. 700 1 $aFERNANDES, F. T. 700 1 $aCARNEIRO, N. P. 700 1 $aCRUZ, I. 700 1 $aCRUZ, I. 773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 43, n. 4, p. 513-520, abr. 2008
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