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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
19/10/2021 |
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Data da última atualização: |
19/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
EVANS, F. F.; ROSADO, A. S.; SEBASTIÁN, G. V.; CASELLA, R.; MACHADO, P. L. O. de A.; HOLMSTRÖM, C.; KJELLEBERG, S.; ELSAS, J. D. van; SELDIN, L. |
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Afiliação: |
FLAVIA F. EVANS, UFRJ; ALEXANDRE S. ROSADO, UFRJ; GINA V. SEBASTIÁN, CENPES/PETROBRAS; RENATA CASELLA, CENPES/PETROBRAS; PEDRO LUIZ OLIVEIRA DE A MACHADO, CNPS; CAROLA HOLMSTRÖM, UNIVERSITY OF NEW SOUTH WALES; STAFFAN KJELLEBERG, UNIVERSITY OF NEW SOUTH WALES; JAN D. VAN ELSAS, GRONINGEN UNIVERSITY; LUCY SELDIN, UFRJ. |
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Título: |
Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
FEMS Microbiology Ecology, v. 49, n. 2, p. 295-305, Aug. 2004. |
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DOI: |
https://doi.org/10.1016/j.femsec.2004.04.007 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Pedro L. O. A. Machado. |
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Conteúdo: |
The aim of this study was to analyse the effect of oil contamination and biostimulation (soil pH raise, and nitrogen, phosphate and sulphur addition) on the diversity of a bacterial community of an acidic Cambisol under Atlantic Forest. The experiment was based on the enumeration of bacterial populations and hydrocarbon degraders in microcosms through the use of conventional plating techniques and molecular fingerprinting of samples directly from the environment. PCR followed by denaturing gradient gel electrophoresis (DGGE) was used to generate microbial community fingerprints employing 16S rRNA gene as molecular marker. Biostimulation led to increases of soil pH (to 7.0) and of the levels of phosphorus and K, Ca, and Mg. Oil contamination caused an increase in soil organic carbon (170-190% higher than control soil). Total bacterial counts were stable throughout the experiment, while MPN counts of hydrocarbon degraders showed an increase in the biostimulated and oil-contaminated soil samples. Molecular fingerprinting performed with 16S rRNA gene PCR and DGGE analysis revealed stable patterns along the 360 days of experiment, showing little change in oil-contaminated microcosms after 90 days. The DGGE patterns of the biostimulated samples showed severe changes due to decreases in the number of bands as compared to the control samples as from 15 days after addition of nutrients to the soil. Results obtained in the present study indicate that the addition of inorganic compounds to soil in conjunction with oil contamination has a greater impact on the bacterial community than oil contamination only. MenosThe aim of this study was to analyse the effect of oil contamination and biostimulation (soil pH raise, and nitrogen, phosphate and sulphur addition) on the diversity of a bacterial community of an acidic Cambisol under Atlantic Forest. The experiment was based on the enumeration of bacterial populations and hydrocarbon degraders in microcosms through the use of conventional plating techniques and molecular fingerprinting of samples directly from the environment. PCR followed by denaturing gradient gel electrophoresis (DGGE) was used to generate microbial community fingerprints employing 16S rRNA gene as molecular marker. Biostimulation led to increases of soil pH (to 7.0) and of the levels of phosphorus and K, Ca, and Mg. Oil contamination caused an increase in soil organic carbon (170-190% higher than control soil). Total bacterial counts were stable throughout the experiment, while MPN counts of hydrocarbon degraders showed an increase in the biostimulated and oil-contaminated soil samples. Molecular fingerprinting performed with 16S rRNA gene PCR and DGGE analysis revealed stable patterns along the 360 days of experiment, showing little change in oil-contaminated microcosms after 90 days. The DGGE patterns of the biostimulated samples showed severe changes due to decreases in the number of bands as compared to the control samples as from 15 days after addition of nutrients to the soil. Results obtained in the present study indicate that the addition of inorganic compound... Mostrar Tudo |
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Palavras-Chave: |
16S rRNA gene; Atlantic forest; Bacterial diversity; Biostimulation; Crude oil; DGGE; Soil microcosms. |
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Thesaurus Nal: |
Cambisols. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227068/1/Impact-of-oil-contamination-and-biostimulation-2004.pdf
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Marc: |
LEADER 02661naa a2200337 a 4500
001 2135450
005 2021-10-19
008 2004 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.femsec.2004.04.007$2DOI
100 1 $aEVANS, F. F.
245 $aImpact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms.$h[electronic resource]
260 $c2004
500 $aNa publicação: Pedro L. O. A. Machado.
520 $aThe aim of this study was to analyse the effect of oil contamination and biostimulation (soil pH raise, and nitrogen, phosphate and sulphur addition) on the diversity of a bacterial community of an acidic Cambisol under Atlantic Forest. The experiment was based on the enumeration of bacterial populations and hydrocarbon degraders in microcosms through the use of conventional plating techniques and molecular fingerprinting of samples directly from the environment. PCR followed by denaturing gradient gel electrophoresis (DGGE) was used to generate microbial community fingerprints employing 16S rRNA gene as molecular marker. Biostimulation led to increases of soil pH (to 7.0) and of the levels of phosphorus and K, Ca, and Mg. Oil contamination caused an increase in soil organic carbon (170-190% higher than control soil). Total bacterial counts were stable throughout the experiment, while MPN counts of hydrocarbon degraders showed an increase in the biostimulated and oil-contaminated soil samples. Molecular fingerprinting performed with 16S rRNA gene PCR and DGGE analysis revealed stable patterns along the 360 days of experiment, showing little change in oil-contaminated microcosms after 90 days. The DGGE patterns of the biostimulated samples showed severe changes due to decreases in the number of bands as compared to the control samples as from 15 days after addition of nutrients to the soil. Results obtained in the present study indicate that the addition of inorganic compounds to soil in conjunction with oil contamination has a greater impact on the bacterial community than oil contamination only.
650 $aCambisols
653 $a16S rRNA gene
653 $aAtlantic forest
653 $aBacterial diversity
653 $aBiostimulation
653 $aCrude oil
653 $aDGGE
653 $aSoil microcosms
700 1 $aROSADO, A. S.
700 1 $aSEBASTIÁN, G. V.
700 1 $aCASELLA, R.
700 1 $aMACHADO, P. L. O. de A.
700 1 $aHOLMSTRÖM, C.
700 1 $aKJELLEBERG, S.
700 1 $aELSAS, J. D. van
700 1 $aSELDIN, L.
773 $tFEMS Microbiology Ecology$gv. 49, n. 2, p. 295-305, Aug. 2004.
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Registro original: |
Embrapa Solos (CNPS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
12/01/2001 |
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Data da última atualização: |
16/02/2012 |
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Autoria: |
BELTRAO, N. E. de M.; BEZERRA, J. R. C.; BARRETO, A. N.; LIMA, E. F.; OLIVEIRA, F. de A.; RAMALHO, F. de S.; SANTANA, J. C. F. de; COSTA, J. N.; MEDEIROS, J. da C.; BEZERRA, J. E. S.; CARVALHO, J. M. F. C.; NÓBREGA, L. B. da; SILVA, L. C.; CARVALHO, L. P. de; AMORIM NETO, M. da S.; LUZ, M. J. da S. E.; SILVA, O. R. R. F. da; BRAGA SOBRINHO, R.; ALMEIDA, R. P. de; CAMPOS, T. G. da S. |
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Título: |
Recomendações técnicas para cultivo do algodoeiro herbáceo de sequeiro e irrigado nas regiões Nordeste e Norte do Brasil. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Campina Grande: EMBRAPA-CNPA, 1994. |
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Páginas: |
73p. |
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Série: |
(EMBRAPA-CNPA. Circular Tecnica, 17) |
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Idioma: |
Português |
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Palavras-Chave: |
Brasil; Cultivation; Cultivo; Nordeste. |
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Thesagro: |
Algodão; Gossypium Hirsutum. |
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Thesaurus NAL: |
cotton. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/54343/1/Circular-Tecnica-17-19930001.pdf
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Marc: |
LEADER 01261nam a2200433 a 4500
001 1271732
005 2012-02-16
008 1994 bl uuuu 00u1 u #d
100 1 $aBELTRAO, N. E. de M.
245 $aRecomendações técnicas para cultivo do algodoeiro herbáceo de sequeiro e irrigado nas regiões Nordeste e Norte do Brasil.
260 $aCampina Grande: EMBRAPA-CNPA$c1994
300 $a73p.
490 $a(EMBRAPA-CNPA. Circular Tecnica, 17)
650 $acotton
650 $aAlgodão
650 $aGossypium Hirsutum
653 $aBrasil
653 $aCultivation
653 $aCultivo
653 $aNordeste
700 1 $aBEZERRA, J. R. C.
700 1 $aBARRETO, A. N.
700 1 $aLIMA, E. F.
700 1 $aOLIVEIRA, F. de A.
700 1 $aRAMALHO, F. de S.
700 1 $aSANTANA, J. C. F. de
700 1 $aCOSTA, J. N.
700 1 $aMEDEIROS, J. da C.
700 1 $aBEZERRA, J. E. S.
700 1 $aCARVALHO, J. M. F. C.
700 1 $aNÓBREGA, L. B. da
700 1 $aSILVA, L. C.
700 1 $aCARVALHO, L. P. de
700 1 $aAMORIM NETO, M. da S.
700 1 $aLUZ, M. J. da S. E.
700 1 $aSILVA, O. R. R. F. da
700 1 $aBRAGA SOBRINHO, R.
700 1 $aALMEIDA, R. P. de
700 1 $aCAMPOS, T. G. da S.
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Embrapa Algodão (CNPA) |
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