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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
29/12/2022 |
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Data da última atualização: |
15/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
KATAYAMA, E.; RODRIGUES, N. A.; BILATTO, S.; CASCIATORI, F. P.; FARINAS, C. S. |
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Afiliação: |
CRISTIANE SANCHEZ FARINAS, CNPDIA. |
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Título: |
Nanocellulose isolation using a thermostable endoglucanase-rich cocktail from Myceliophthora thermophila cultivated in a multilayer packed-bed bioreactor. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Biomass Conversion and Biorefnery, v. 14, n. 8, 2022. |
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Páginas: |
9121-9136 |
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DOI: |
https://doi.org/10.1007/s13399-022-02977-1 |
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Idioma: |
Inglês |
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Conteúdo: |
The isolation of nanocellulose materials by means of enzymatic hydrolysis ofers advantages in terms of their properties and operational conditions, but there is still a lack of enzymatic cocktails specifcally designed for this application. The present work investigates the use of a thermostable endoglucanase-rich enzymatic cocktail from Myceliophthora thermophila for nanocellulose production. An initial set of experiments was conducted to determine the optimal conditions for enzyme production under solid-state cultivation in small-scale polypropylene plastic bags. A full factorial experimental design was used as a statistical tool to evaluate the efects of the solid substrate and moisture content on the production of endoglucanase and ?-glucosidase. Solid-state cultivations in a multilayer packed-bed bioreactor were then carried out under the selected conditions to obtain a larger volume of an enzymatic extract with high endoglucanase selectivity, given by the ratio of endoglucanase (203±6 U/g-substrate) to ?-glucosidase activity (1.6±0.1 U/g-substrate). The enzymatic hydrolysis of eucalyptus cellulose pulp at diferent temperatures, with this endoglucanase-rich cocktail at a loading of 10 mg-protein/g-pulp, resulted in higher glucose release (11±2 g-glucose/L) at 60 °C, with 61±9% cellulose conversion and 16.2% nanocellulose yield. The isolated nanomaterial with average diameter of 36.65 nm and length of 156.63 nm, presented a crystallinity index of 77.4% and thermal stability temperature of 316 °C. These results show the potential to isolate nanocellulose using a thermostable endoglucanase-rich enzymatic cocktail produced locally by solid-state cultivation in a packed-bed bioreactor, in accordance to the biorefnery concept. MenosThe isolation of nanocellulose materials by means of enzymatic hydrolysis ofers advantages in terms of their properties and operational conditions, but there is still a lack of enzymatic cocktails specifcally designed for this application. The present work investigates the use of a thermostable endoglucanase-rich enzymatic cocktail from Myceliophthora thermophila for nanocellulose production. An initial set of experiments was conducted to determine the optimal conditions for enzyme production under solid-state cultivation in small-scale polypropylene plastic bags. A full factorial experimental design was used as a statistical tool to evaluate the efects of the solid substrate and moisture content on the production of endoglucanase and ?-glucosidase. Solid-state cultivations in a multilayer packed-bed bioreactor were then carried out under the selected conditions to obtain a larger volume of an enzymatic extract with high endoglucanase selectivity, given by the ratio of endoglucanase (203±6 U/g-substrate) to ?-glucosidase activity (1.6±0.1 U/g-substrate). The enzymatic hydrolysis of eucalyptus cellulose pulp at diferent temperatures, with this endoglucanase-rich cocktail at a loading of 10 mg-protein/g-pulp, resulted in higher glucose release (11±2 g-glucose/L) at 60 °C, with 61±9% cellulose conversion and 16.2% nanocellulose yield. The isolated nanomaterial with average diameter of 36.65 nm and length of 156.63 nm, presented a crystallinity index of 77.4% and thermal stabili... Mostrar Tudo |
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Palavras-Chave: |
Endoglucanase; Packed-bed bioreactor; Solid-state cultivation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02537naa a2200229 a 4500
001 2150493
005 2024-05-15
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s13399-022-02977-1$2DOI
100 1 $aKATAYAMA, E.
245 $aNanocellulose isolation using a thermostable endoglucanase-rich cocktail from Myceliophthora thermophila cultivated in a multilayer packed-bed bioreactor.$h[electronic resource]
260 $c2022
300 $a9121-9136
520 $aThe isolation of nanocellulose materials by means of enzymatic hydrolysis ofers advantages in terms of their properties and operational conditions, but there is still a lack of enzymatic cocktails specifcally designed for this application. The present work investigates the use of a thermostable endoglucanase-rich enzymatic cocktail from Myceliophthora thermophila for nanocellulose production. An initial set of experiments was conducted to determine the optimal conditions for enzyme production under solid-state cultivation in small-scale polypropylene plastic bags. A full factorial experimental design was used as a statistical tool to evaluate the efects of the solid substrate and moisture content on the production of endoglucanase and ?-glucosidase. Solid-state cultivations in a multilayer packed-bed bioreactor were then carried out under the selected conditions to obtain a larger volume of an enzymatic extract with high endoglucanase selectivity, given by the ratio of endoglucanase (203±6 U/g-substrate) to ?-glucosidase activity (1.6±0.1 U/g-substrate). The enzymatic hydrolysis of eucalyptus cellulose pulp at diferent temperatures, with this endoglucanase-rich cocktail at a loading of 10 mg-protein/g-pulp, resulted in higher glucose release (11±2 g-glucose/L) at 60 °C, with 61±9% cellulose conversion and 16.2% nanocellulose yield. The isolated nanomaterial with average diameter of 36.65 nm and length of 156.63 nm, presented a crystallinity index of 77.4% and thermal stability temperature of 316 °C. These results show the potential to isolate nanocellulose using a thermostable endoglucanase-rich enzymatic cocktail produced locally by solid-state cultivation in a packed-bed bioreactor, in accordance to the biorefnery concept.
653 $aEndoglucanase
653 $aPacked-bed bioreactor
653 $aSolid-state cultivation
700 1 $aRODRIGUES, N. A.
700 1 $aBILATTO, S.
700 1 $aCASCIATORI, F. P.
700 1 $aFARINAS, C. S.
773 $tBiomass Conversion and Biorefnery$gv. 14, n. 8, 2022.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
03/03/2016 |
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Data da última atualização: |
05/04/2024 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ARBEX, W. A.; SANTOS, K. C. L. dos; NASCIMENTO, E. de O.; SANTOS, G. B. dos; CARVALHO, C. dos S. B. de; CARVALHO, B. C. de. |
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Afiliação: |
WAGNER ANTONIO ARBEX, CNPGL; KATIA CRISTINA LAGE DOS SANTOS, CNPGL; ÊNIO DE O. NASCIMENTO; GUSTAVO B. DOS SANTOS; CAIO DOS S. B. DE CARVALHO; BRUNO CAMPOS DE CARVALHO, CNPGL. |
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Título: |
Computação móvel aplicada à pecuária de precisão para a determinação do escore da condição corporal. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE AGROINFORMÁTICA, 10., 2015, Ponta Grossa. Anais... Ponta Grossa: SBIAgro, 2015. |
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Páginas: |
9 p. |
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Idioma: |
Português |
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Palavras-Chave: |
Dispositivo móvel; E-score; ECC; Visão computacional; Zootecnia de precisão. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1039578/1/Computacao-movel-aplicada-a-pecuaria.pdf
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Marc: |
LEADER 00786nam a2200229 a 4500
001 2039578
005 2024-04-05
008 2015 bl uuuu u00u1 u #d
100 1 $aARBEX, W. A.
245 $aComputação móvel aplicada à pecuária de precisão para a determinação do escore da condição corporal.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE AGROINFORMÁTICA, 10., 2015, Ponta Grossa. Anais... Ponta Grossa: SBIAgro$c2015
300 $a9 p.
653 $aDispositivo móvel
653 $aE-score
653 $aECC
653 $aVisão computacional
653 $aZootecnia de precisão
700 1 $aSANTOS, K. C. L. dos
700 1 $aNASCIMENTO, E. de O.
700 1 $aSANTOS, G. B. dos
700 1 $aCARVALHO, C. dos S. B. de
700 1 $aCARVALHO, B. C. de
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