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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
21/08/2012 |
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Data da última atualização: |
23/10/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DALTRO, C. B.; PEREIRA, Á. J.; ALFENAS-ZERBINI, P.; BEZERRA-JUNIOR, J. E. A.; LIMA, J. A. de A.; ZERBINI, F. M.; ANDRADE, E. C. de; CASCARDO, R. de S. |
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Afiliação: |
CLEIDIANE BORGES DALTRO, UFRB; ÁLVARO JÚLIO PEREIRA, UFV; POLIANE ALFENAS-ZERBINI, UFV; JOSÉ EVANDO AGUIAR BEZERRA-JUNIOR, UFC; JOSÉ ALBÉRSIO DE ARAÚJO LIMA, UFC; FRANCISCO MURILO ZERBINI, UFV; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; RENAN DE SOUZA CASCARDO, UFRJ. |
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Título: |
Genetic variability of papaya lethal yellowing virus isolates from Ceará and Rio Grande do Norte states, Brazil. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 37, n. 1, p. 37-43, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The papaya (Carica papaya) is a fruit crop of great economic importance throughout the Brazilian northeast, which is responsible for 60% of the national output. Papayas in the states of Ceará and Rio Grande do Norte are affected by lethal yellowing disease, caused by papaya lethal yellowing virus (PLYV). Previous work suggested that PLYV is a putative sobemovirus. To assess the genetic variability of PLYV, foliar samples were collected in October 2008 and October 2009 in commercial fields from Ceará and Rio Grande do Norte states, and total RNA was extracted. Specific primers based on the sequence of a previously characterized PLYV isolate were used for the RT-PCR-based amplification of a 900 bp fragment corresponding to the central region of the viral genome. Fragments from 21 viral isolates were cloned and sequenced. Sequence analyses indicated >97% nucleotide sequence identity among the isolates, 94-100% identity with the previously sequenced PLYV isolate, and a lower but significant identity with sobemoviruses (43-48.5%). These results suggest a low genetic variability among PLYV isolates, and are in agreement with the provisional placement of PLYV in the genus Sobemovirus. Definitive taxonomic conclusions, however, can only be drawn after the determination of the full-length genomic sequence. |
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Palavras-Chave: |
Papaya; PLYV. |
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Thesagro: |
Carica Papaya. |
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Thesaurus Nal: |
Sobemovirus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/64585/1/Genetic-variability-of-Papaya-lethal-yellowing-virus-isolates-from-Ceara-and-Rio-Grande-do-Norte-states-Brazil..pdf
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Marc: |
LEADER 02058naa a2200253 a 4500
001 1931832
005 2012-10-23
008 2012 bl uuuu u00u1 u #d
100 1 $aDALTRO, C. B.
245 $aGenetic variability of papaya lethal yellowing virus isolates from Ceará and Rio Grande do Norte states, Brazil.
260 $c2012
520 $aThe papaya (Carica papaya) is a fruit crop of great economic importance throughout the Brazilian northeast, which is responsible for 60% of the national output. Papayas in the states of Ceará and Rio Grande do Norte are affected by lethal yellowing disease, caused by papaya lethal yellowing virus (PLYV). Previous work suggested that PLYV is a putative sobemovirus. To assess the genetic variability of PLYV, foliar samples were collected in October 2008 and October 2009 in commercial fields from Ceará and Rio Grande do Norte states, and total RNA was extracted. Specific primers based on the sequence of a previously characterized PLYV isolate were used for the RT-PCR-based amplification of a 900 bp fragment corresponding to the central region of the viral genome. Fragments from 21 viral isolates were cloned and sequenced. Sequence analyses indicated >97% nucleotide sequence identity among the isolates, 94-100% identity with the previously sequenced PLYV isolate, and a lower but significant identity with sobemoviruses (43-48.5%). These results suggest a low genetic variability among PLYV isolates, and are in agreement with the provisional placement of PLYV in the genus Sobemovirus. Definitive taxonomic conclusions, however, can only be drawn after the determination of the full-length genomic sequence.
650 $aSobemovirus
650 $aCarica Papaya
653 $aPapaya
653 $aPLYV
700 1 $aPEREIRA, Á. J.
700 1 $aALFENAS-ZERBINI, P.
700 1 $aBEZERRA-JUNIOR, J. E. A.
700 1 $aLIMA, J. A. de A.
700 1 $aZERBINI, F. M.
700 1 $aANDRADE, E. C. de
700 1 $aCASCARDO, R. de S.
773 $tTropical Plant Pathology$gv. 37, n. 1, p. 37-43, 2012.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 3 | |
| 1. |  | ALMEIDA, J. C. F. de; XAVIER, A. da S.; CASCARDO, R. de S.; REZENDE, R. R. de; SOUZA, F. O. de; LOPES, C. A.; ALFENAS-ZERBINI, P. Genomic and biological characterization of Ralstonia solanacearum inovirus Brazil 1, an inovirus that alters the pathogenicity of the Phytopathogen Ralstonia pseudosolanacearum. Microbial Ecology, v. 84, p. 527-538, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Hortaliças. |
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| 2. | | DALTRO, C. B.; PEREIRA, Á. J.; ALFENAS-ZERBINI, P.; BEZERRA-JUNIOR, J. E. A.; LIMA, J. A. de A.; ZERBINI, F. M.; ANDRADE, E. C. de; CASCARDO, R. de S. Genetic variability of papaya lethal yellowing virus isolates from Ceará and Rio Grande do Norte states, Brazil. Tropical Plant Pathology, v. 37, n. 1, p. 37-43, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|  |
| 3. | | HERMENEGILDO, P. da S.; FREITAS, R. G. de; CASCARDO, R. de S; GUIMARÃES, L. M. S.; BADEL, J. L.; ALFENAS‑ZERBINI, P.; MARQUES, A. S. dos A.; ALFENAS, A. C.; FERREIRA, M. A. S. V. Sensitive detection, quantification, and monitoring of Erwinia psidii colonization of guava plants using intercalating dye-based real-time PCR. Tropical Plant Pathology, v. 48, p. 375-383, 2022. Na publicação: Abi S. A. Marques.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 3 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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