Data/hora: 01/06/2024 05:49:15

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	26/04/2010	
Data da última atualização: 	16/02/2024	
Autoria: 	STEINBERG, R. S.; PEREIRA, L.; LACORTE, G. A.; PEIXOTO, M. G. C. D.; VERNEQUE, R. da S.; TEODORO, R. L.; MACHADO, M. A.; FONSECA, C. G.; CARVALHO, M. R. S.	
Afiliação: 	R. S. STEINBERG, UFMG; L. PEREIRA, UFMG; G. A. LACORTE, UFMG; MARIA GABRIELA CAMPOLINA D PEIXOTO, CNPGL; RUI DA SILVA VERNEQUE, CNPGL; ROBERTO LUIZ TEODORO, Pesquisador aposentado do CNPGL; MARCO ANTONIO MACHADO, CNPGL; C. G. FONSECA, UFMG; M. R. S. CARVALHO, UFMG.	
Título: 	Technical note: A new and cost-effective method for detection of the bovine acyl-CoA: diacyglycerol acyltransferase 1 K232A polymorphism in cattle.	
Ano de publicação: 	2009	
Fonte/Imprenta: 	Journal of Dairy Science, v. 92, n. 2, p. 773-776, 2009.	
Idioma: 	Inglês	
Conteúdo: 	A new, quick, and inexpensive method for detecting the bovine acyl-CoA:diacylglycerol acyltransferase1 (DGAT1) polymorphism (K232A) through tetra-primer amplification refractory mutation system by PCR (ARMS-PCR) was developed in the present investigation. The DGAT1 gene was recently identified as underlying variation in milk production traits. To date, PCR techniques such as PCR-RFLP have been used for detecting the DGAT1 K232A polymorphism, despite being expensive and laborious. The method proposed here, a tetra-primer ARMS-PCR, showed 100% sensitivity and specificity when compared with PCR-RFLP results obtained in a sample of 80 animals tested in a double-blind system. Our results suggest that the use of tetra-primer ARMS-PCR for DGAT1 K232A polymorphism genotyping could greatly reduce costs providing information for both research purposes and for dairy cattle breeders who perform DGAT1 genotyping for gene-assisted selection.	
Palavras-Chave: 	DGAT1.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/711762/1/Technical-note-A-new-and-cost-effective-method-for-detection-of-the-bovine-acyl-CoA.pdf	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (17045-1) - UPC - AP - DD -  -  -  - DIS