Data/hora: 02/06/2024 16:06:13

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 26/01/2015
Data da última atualização: | 05/02/2024
Autoria: | FONSECA, P. A. S; ROSSE, I. C.; DEMIRANDA, M.; MACHADO, M. A.; VERNEQUE, R. da S.; PEIXOTO, M. G. C. D.; CARVALHO, M. R. S.
Afiliação: | P. A. S. FONSECA, UFMG; I. C. ROSSE, UFMG; M. DEMIRANDA, UFMG; MARCO ANTONIO MACHADO, CNPGL; RUI DA SILVA VERNEQUE, CNPGL; MARIA GABRIELA CAMPOLINA D PEIXOTO, CNPGL; M. R. S. CARVALHO, UFMG.
Título: | A new tetra-primer ARMS-PCR for genotyping bovine kappa-casein polymorphisms.
Ano de publicação: | 2014
Fonte/Imprenta: | Genetics and Molecular Research, v. 12, n. 4, p. 6521-6526, 2014.
Idioma: | Inglês
Conteúdo: | Kappa-casein (κ-casein) is one of the most abundant milk proteins. Its main function is to avoid the aggregation of casein micelles, keeping them, and therefore calcium phosphate, in pockets in solution. In bovines, a κ-casein functional polymorphism has been associated with fat, calcium, and protein milk contents and faster curd contraction in cheese production. Quicker curd contraction reduces the loss of milk solids, enhancing cheese yield. This polymorphism induces a double amino acid substitution (Thr136Ile and Ala148Asp). The polymorphism is normally detected by PCR-RFLP, which is a laborious method. An interesting methodological alternative is the tetra-primer amplification refractory mutation system PCR (tetra-primer ARMS-PCR). A tetra-primer ARMS-PCR for the detection of this κ-casein polymorphism has been described. However, specificity was not achieved, probably due to problems with primer design. We developed a new tetra-primer ARMS-PCR for the detection of the κ-casein polymorphism. This new method was validated in a double-blind test, by comparison with the results obtained for 50 Guzerá bulls formerly genotyped by PCR-RFLP. This new method achieved 100% sensitivity and specificity. We conclude that this method is a useful, cost-efficient alternative for the detection of functional κ-casein polymorphisms.
Palavras-Chave: | CSN3; K-Casein; K-Casein polymorphism; Tetra-primer ARMS-PCR.
Thesaurus Nal: | casein.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (21624-1) - UPC - AP - DD -  -  -  - DIS