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Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
16/07/2010 |
Data da última atualização: |
20/07/2010 |
Autoria: |
PÁDUA, G. P. de; FRANCA NETO, J. de B.; CARVALHO, M. L. M. de; COSTA, O.; KRZYZANOWSKI, F. C.; COSTA, N. P. da. |
Afiliação: |
GILDA PIZZOLANTE DE PÁDUA, EMBRAPA - EPAMIG; JOSE DE BARROS FRANCA NETO, CNPSo; MARIA LAENE MOREIRA DE CARVALHO, UFLA; ODAIR COSTA, Sementes Adriana; FRANCISCO CARLOS KRZYZANOWSKI, CNPSo; NILTON PEREIRA DA COSTA, CNPSo. |
Título: |
Tolerance level of green seed in soybean seed lots after storage. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
Revista Brasileira de Sementes, Londrina, v. 29, n. 3, p. 128-138, dez. 2007. |
DOI: |
10.1590/S0101-31222007000300015 |
Idioma: |
Português |
Palavras-Chave: |
Sementes esverdeadas. |
Thesagro: |
Armazenamento; Soja. |
Categoria do assunto: |
-- |
URL: |
https://www.scielo.br/scielo.php?script=sci_pdf&pid=S0101-31222007000300015&lng=pt&nrm=iso&tlng=en
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Marc: |
LEADER 00648nam a2200205 a 4500 001 1858020 005 2010-07-20 008 2007 bl uuuu u0uu1 u #d 024 7 $a10.1590/S0101-31222007000300015$2DOI 100 1 $aPÁDUA, G. P. de 245 $aTolerance level of green seed in soybean seed lots after storage. 260 $aRevista Brasileira de Sementes, Londrina, v. 29, n. 3, p. 128-138, dez. 2007.$c2007 650 $aArmazenamento 650 $aSoja 653 $aSementes esverdeadas 700 1 $aFRANCA NETO, J. de B. 700 1 $aCARVALHO, M. L. M. de 700 1 $aCOSTA, O. 700 1 $aKRZYZANOWSKI, F. C. 700 1 $aCOSTA, N. P. da
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Embrapa Soja (CNPSO) |
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Registro Completo
Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
02/12/2014 |
Data da última atualização: |
02/12/2014 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
FERNANDES, M. F.; SAXSENA, J.; DICK, R. P. |
Afiliação: |
MARCELO FERREIRA FERNANDES, CPATC. |
Título: |
Comparison of whole-cell fatty acid (MIDI) or phospholipid fatty acid (PLFA) extractants as biomarkers to profile soil microbial communities. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Microbial Ecology, v. 66, p. 145-157, 2013. |
DOI: |
10.1007/s00248-013-0195-2 |
Idioma: |
Inglês |
Conteúdo: |
The whole-cell lipid extraction to profile micro-bial communities on soils using fatty acid (FA) biomarkers is commonly done with the two extractants associated with the hospholipid fatty acid (PLFA) or Microbial IDentifica-tion Inc. (MIDI) methods. These extractants have very dif-ferent chemistry and lipid separation procedures, but often shown a similar ability to discriminate soils from various management and vegetation systems. However, the mecha-nism and the chemistry of the exact suite of FAs extracted by these two methods are poorly understood. Therefore, the objective was to qualitatively and quantitatively compare the MIDI and PLFA microbial profiling methods for detect-ing microbial community shifts due to soil type or manage-ment. Twenty-nine soil samples were collected from a wide range of soil types across Oregon and extracted FAs by each method were analyzed by gas chromatography (GC) and GC-mass spectrometry. Unlike PLFA profiles, which were highly related to microbial FAs, the overall MIDI-FA pro-files were highly related to the plant-derived FAs. Plant-associated compounds were quantitatively related to partic-ulate organic matter (POM) and qualitatively related to the standing vegetation at sampling. These FAs were negatively correlated to respiration rate normalized to POM (Resp POM), which increased in systems under more intensive manage-ment. A strong negative correlation was found between MIDI-FA to PLFA ratios and total organic carbon (TOC). When the reagents used in MIDI procedure were tested for the limited recovery of MIDI-FAs from soil with high or-ganic matter, the recovery of MIDI-FA microbial signatures sharply decreased with increasing ratios of soil to extractant. Hence, the MIDI method should be used with great caution for interpreting changes in FA profiles due to shifts in microbial communities. MenosThe whole-cell lipid extraction to profile micro-bial communities on soils using fatty acid (FA) biomarkers is commonly done with the two extractants associated with the hospholipid fatty acid (PLFA) or Microbial IDentifica-tion Inc. (MIDI) methods. These extractants have very dif-ferent chemistry and lipid separation procedures, but often shown a similar ability to discriminate soils from various management and vegetation systems. However, the mecha-nism and the chemistry of the exact suite of FAs extracted by these two methods are poorly understood. Therefore, the objective was to qualitatively and quantitatively compare the MIDI and PLFA microbial profiling methods for detect-ing microbial community shifts due to soil type or manage-ment. Twenty-nine soil samples were collected from a wide range of soil types across Oregon and extracted FAs by each method were analyzed by gas chromatography (GC) and GC-mass spectrometry. Unlike PLFA profiles, which were highly related to microbial FAs, the overall MIDI-FA pro-files were highly related to the plant-derived FAs. Plant-associated compounds were quantitatively related to partic-ulate organic matter (POM) and qualitatively related to the standing vegetation at sampling. These FAs were negatively correlated to respiration rate normalized to POM (Resp POM), which increased in systems under more intensive manage-ment. A strong negative correlation was found between MIDI-FA to PLFA ratios and total organic carbon (TOC). When the r... Mostrar Tudo |
Palavras-Chave: |
Lipídios. |
Thesagro: |
Microbiologia; Solo. |
Thesaurus NAL: |
soil. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02496naa a2200205 a 4500 001 2001362 005 2014-12-02 008 2013 bl uuuu u00u1 u #d 024 7 $a10.1007/s00248-013-0195-2$2DOI 100 1 $aFERNANDES, M. F. 245 $aComparison of whole-cell fatty acid (MIDI) or phospholipid fatty acid (PLFA) extractants as biomarkers to profile soil microbial communities.$h[electronic resource] 260 $c2013 520 $aThe whole-cell lipid extraction to profile micro-bial communities on soils using fatty acid (FA) biomarkers is commonly done with the two extractants associated with the hospholipid fatty acid (PLFA) or Microbial IDentifica-tion Inc. (MIDI) methods. These extractants have very dif-ferent chemistry and lipid separation procedures, but often shown a similar ability to discriminate soils from various management and vegetation systems. However, the mecha-nism and the chemistry of the exact suite of FAs extracted by these two methods are poorly understood. Therefore, the objective was to qualitatively and quantitatively compare the MIDI and PLFA microbial profiling methods for detect-ing microbial community shifts due to soil type or manage-ment. Twenty-nine soil samples were collected from a wide range of soil types across Oregon and extracted FAs by each method were analyzed by gas chromatography (GC) and GC-mass spectrometry. Unlike PLFA profiles, which were highly related to microbial FAs, the overall MIDI-FA pro-files were highly related to the plant-derived FAs. Plant-associated compounds were quantitatively related to partic-ulate organic matter (POM) and qualitatively related to the standing vegetation at sampling. These FAs were negatively correlated to respiration rate normalized to POM (Resp POM), which increased in systems under more intensive manage-ment. A strong negative correlation was found between MIDI-FA to PLFA ratios and total organic carbon (TOC). When the reagents used in MIDI procedure were tested for the limited recovery of MIDI-FAs from soil with high or-ganic matter, the recovery of MIDI-FA microbial signatures sharply decreased with increasing ratios of soil to extractant. Hence, the MIDI method should be used with great caution for interpreting changes in FA profiles due to shifts in microbial communities. 650 $asoil 650 $aMicrobiologia 650 $aSolo 653 $aLipídios 700 1 $aSAXSENA, J. 700 1 $aDICK, R. P. 773 $tMicrobial Ecology$gv. 66, p. 145-157, 2013.
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