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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
16/02/2017 |
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Data da última atualização: |
20/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FACCIN, H.; LOOSE, R. F.; VIANA, C.; LAMEIRA, O. A.; CARVALHO, L. M. de. |
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Afiliação: |
Henrique Faccin, UFSM; Roberta Fabricio Loose, UFSM; Carine Viana, UFSM; OSMAR ALVES LAMEIRA, CPATU; Leandro Machado de Carvalho, UFSM. |
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Título: |
Determination of phenolic compounds in extracts of Amazonian medicinal plants by liquid chromatography-electrospray tandem mass spectrometry. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Analytical Methods, v. 9, n. 7, p. 1141-1151, Feb. 2017. |
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DOI: |
10.1039/C6AY02937J |
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Idioma: |
Inglês |
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Conteúdo: |
A method for the separation, identification and quantification of 24 phenolic compounds using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was developed and validated. Six species of traditional medicinal plants from the Brazilian Amazon region were studied (Mansoa alliacea, Bauhinia variegata var. variegata, Bauhinia variegata var. alboflava, Connarus perrottetii var. angustifolius, Cecropia obtusa and Cecropia palmata). The analytes were separated by a reversed-phase SB-C18 column (2.1 × 50 mm, 1.8 μm) using a gradient elution of 7 min composed of 0.1% acetic acid in water (v/v) and acetonitrile, at a constant flow rate of 0.8 mL min−1. The limit of detection for the analytes ranged between 0.5 and 130.3 μg L−1. Intra- and inter-day precision showed satisfactory results and the recoveries obtained for the 24 analytes varied between 91.7 and 111.9% for most of the evaluated matrices. Quantifications were performed with the standard addition method (r > 0.99) using the data acquired in a multiple reaction monitoring (MRM) mode. Rutin, apigenin, vanillic acid, caffeic acid, ferulic acid, p-coumaric acid and trans-cinnamic acid were found in all the studied plants. However, we highlight the high contents of rutin in varieties of Bauhinia variegata (up to 2.52 mg g−1 of plant), chlorogenic acid in species of genus Cecropia (up to 0.57 mg g−1 of plant) and catechin in Connarus perrottetii var. angustifolius (1.77 mg g−1 of plant). MenosA method for the separation, identification and quantification of 24 phenolic compounds using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was developed and validated. Six species of traditional medicinal plants from the Brazilian Amazon region were studied (Mansoa alliacea, Bauhinia variegata var. variegata, Bauhinia variegata var. alboflava, Connarus perrottetii var. angustifolius, Cecropia obtusa and Cecropia palmata). The analytes were separated by a reversed-phase SB-C18 column (2.1 × 50 mm, 1.8 μm) using a gradient elution of 7 min composed of 0.1% acetic acid in water (v/v) and acetonitrile, at a constant flow rate of 0.8 mL min−1. The limit of detection for the analytes ranged between 0.5 and 130.3 μg L−1. Intra- and inter-day precision showed satisfactory results and the recoveries obtained for the 24 analytes varied between 91.7 and 111.9% for most of the evaluated matrices. Quantifications were performed with the standard addition method (r > 0.99) using the data acquired in a multiple reaction monitoring (MRM) mode. Rutin, apigenin, vanillic acid, caffeic acid, ferulic acid, p-coumaric acid and trans-cinnamic acid were found in all the studied plants. However, we highlight the high contents of rutin in varieties of Bauhinia variegata (up to 2.52 mg g−1 of plant), chlorogenic acid in species of genus Cecropia (up to 0.57 mg g−1 of plant) and catechin in Connarus perrottetii var. an... Mostrar Tudo |
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Thesagro: |
Planta Medicinal. |
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Thesaurus Nal: |
Amazonia. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02203naa a2200205 a 4500
001 2064445
005 2022-05-20
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1039/C6AY02937J$2DOI
100 1 $aFACCIN, H.
245 $aDetermination of phenolic compounds in extracts of Amazonian medicinal plants by liquid chromatography-electrospray tandem mass spectrometry.$h[electronic resource]
260 $c2017
520 $aA method for the separation, identification and quantification of 24 phenolic compounds using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was developed and validated. Six species of traditional medicinal plants from the Brazilian Amazon region were studied (Mansoa alliacea, Bauhinia variegata var. variegata, Bauhinia variegata var. alboflava, Connarus perrottetii var. angustifolius, Cecropia obtusa and Cecropia palmata). The analytes were separated by a reversed-phase SB-C18 column (2.1 × 50 mm, 1.8 μm) using a gradient elution of 7 min composed of 0.1% acetic acid in water (v/v) and acetonitrile, at a constant flow rate of 0.8 mL min−1. The limit of detection for the analytes ranged between 0.5 and 130.3 μg L−1. Intra- and inter-day precision showed satisfactory results and the recoveries obtained for the 24 analytes varied between 91.7 and 111.9% for most of the evaluated matrices. Quantifications were performed with the standard addition method (r > 0.99) using the data acquired in a multiple reaction monitoring (MRM) mode. Rutin, apigenin, vanillic acid, caffeic acid, ferulic acid, p-coumaric acid and trans-cinnamic acid were found in all the studied plants. However, we highlight the high contents of rutin in varieties of Bauhinia variegata (up to 2.52 mg g−1 of plant), chlorogenic acid in species of genus Cecropia (up to 0.57 mg g−1 of plant) and catechin in Connarus perrottetii var. angustifolius (1.77 mg g−1 of plant).
650 $aAmazonia
650 $aPlanta Medicinal
700 1 $aLOOSE, R. F.
700 1 $aVIANA, C.
700 1 $aLAMEIRA, O. A.
700 1 $aCARVALHO, L. M. de
773 $tAnalytical Methods$gv. 9, n. 7, p. 1141-1151, Feb. 2017.
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