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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
15/01/2009 |
Data da última atualização: |
21/03/2023 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
CARVALHO, G. D.; NASCIMENTO, J. B.; CUNHA, E. de Q.; FERREIRA, E. P. de B.; STONE, L. F.; OLIVEIRA, J. P. de; DIDONET, A. D. |
Afiliação: |
GLAUCILENE DUARTE CARVALHO, estagiária CNPAF; JACQUELINE BARBOSA NASCIMENTO, estagiária CNPAF; EURÂIMI DE QUEIROZ CUNHA, doutorando Universidade Federal de Goiás; ENDERSON PETRONIO DE BRITO FERREIRA, CNPAF; LUIS FERNANDO STONE, CNPAF; JAISON PEREIRA DE OLIVEIRA, CNPAF; AGOSTINHO DIRCEU DIDONET, CNPAF. |
Título: |
Efeito de adubos verdes e sistemas de manejo na compactação de um solo cultivado com feijoeiro comum. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 9., 2008, Campinas. Ciência e tecnologia na cadeia produtiva do feijão. Campinas: Instituto Agronômico, 2008. |
Série: |
(IAC. Documentos, 85). |
Idioma: |
Português |
Conteúdo: |
O presente trabalho teve como objetivo avaliar o efeito da adubação verde sob a compactação do solo e sob o rendimento do feijão cultivado em sistema de plantio direto e convencional, na região de Santo Antônio de Goiás, GO. |
Palavras-Chave: |
CONAFE. |
Thesagro: |
Adubo Verde; Cerrado; Feijão; Phaseolus Vulgaris; Plantio Direto. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/74643/1/234.pdf
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Marc: |
LEADER 01124nam a2200265 a 4500 001 1216167 005 2023-03-21 008 2008 bl uuuu u00u1 u #d 100 1 $aCARVALHO, G. D. 245 $aEfeito de adubos verdes e sistemas de manejo na compactação de um solo cultivado com feijoeiro comum.$h[electronic resource] 260 $aIn: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 9., 2008, Campinas. Ciência e tecnologia na cadeia produtiva do feijão. Campinas: Instituto Agronômico$c2008 490 $a(IAC. Documentos, 85). 520 $aO presente trabalho teve como objetivo avaliar o efeito da adubação verde sob a compactação do solo e sob o rendimento do feijão cultivado em sistema de plantio direto e convencional, na região de Santo Antônio de Goiás, GO. 650 $aAdubo Verde 650 $aCerrado 650 $aFeijão 650 $aPhaseolus Vulgaris 650 $aPlantio Direto 653 $aCONAFE 700 1 $aNASCIMENTO, J. B. 700 1 $aCUNHA, E. de Q. 700 1 $aFERREIRA, E. P. de B. 700 1 $aSTONE, L. F. 700 1 $aOLIVEIRA, J. P. de 700 1 $aDIDONET, A. D.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
11/12/2020 |
Data da última atualização: |
11/12/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
MARQUES, T. C.; SANTOS, E. C. da S.; DIESEL, T. O.; MARTINS, C. F.; CUMPA, H. C. B.; LEME, L. de O.; DODE, M. A. N.; ALVES, B. G.; COSTA, F. P. H.; OLIVEIRA, E. B.; GAMBARINI, M. L. |
Afiliação: |
CARLOS FREDERICO MARTINS, CPAC; MARGOT ALVES NUNES DODE, Cenargen. |
Título: |
Blastocoel fluid removal and melatonin supplementation in the culture medium improve the viability of vitrified bovine embryos. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Theriogenology, Vv. 160, p. 134-141, 2021. |
Páginas: |
p. 134-141 |
ISSN: |
0093-691X |
Idioma: |
Português |
Conteúdo: |
In this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased HSPA5 (P ¼ 0.0118) expression and did not affect SLC2A3, MSH6, KRT8, and FOSL1 expression (P > 0.05). In conclusion, melatonin (109 M) supplementation in the culture medium and BFR on D7 of culture increased the hatching rate 24, 48, and 72 h after warming of the vitrified embryos, indicating an improvement in cryotolerance. MenosIn this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased ... Mostrar Tudo |
Palavras-Chave: |
Apoptose celular; Criotolerância; Fertilização; Melatonina; Vitrificação. |
Thesagro: |
Cultura de Embrião; Embrião. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02843naa a2200349 a 4500 001 2127986 005 2020-12-11 008 2021 bl uuuu u00u1 u #d 022 $a0093-691X 100 1 $aMARQUES, T. C. 245 $aBlastocoel fluid removal and melatonin supplementation in the culture medium improve the viability of vitrified bovine embryos.$h[electronic resource] 260 $c2021 300 $ap. 134-141 520 $aIn this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased HSPA5 (P ¼ 0.0118) expression and did not affect SLC2A3, MSH6, KRT8, and FOSL1 expression (P > 0.05). In conclusion, melatonin (109 M) supplementation in the culture medium and BFR on D7 of culture increased the hatching rate 24, 48, and 72 h after warming of the vitrified embryos, indicating an improvement in cryotolerance. 650 $aCultura de Embrião 650 $aEmbrião 653 $aApoptose celular 653 $aCriotolerância 653 $aFertilização 653 $aMelatonina 653 $aVitrificação 700 1 $aSANTOS, E. C. da S. 700 1 $aDIESEL, T. O. 700 1 $aMARTINS, C. F. 700 1 $aCUMPA, H. C. B. 700 1 $aLEME, L. de O. 700 1 $aDODE, M. A. N. 700 1 $aALVES, B. G. 700 1 $aCOSTA, F. P. H. 700 1 $aOLIVEIRA, E. B. 700 1 $aGAMBARINI, M. L. 773 $tTheriogenology, Vv. 160, p. 134-141, 2021.
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