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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
14/10/2014 |
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Data da última atualização: |
23/01/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ZULETA, L. F. G.; CUNHA, C. de O.; CARVALHO, F. M. de; CIAPINA, L. P.; SOUZA, R. C; MERCANTE, F. M.; FARIA, S. M. de; BALDANI, J. I.; STRALIOTTO, R.; HUNGRIA, M.; VASCONCELOS, A. T. R. de. |
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Afiliação: |
SERGIO MIANA DE FARIA, CNPAB; JOSE IVO BALDANI, CNPAB; ROSANGELA STRALIOTTO, CNPAB. |
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Título: |
The complete genome of Burkholderia phenoliruptrix strain BR3459a, a symbiont of Mimosa flocculosa: highlighting the coexistence of symbiotic and pathogenic genes. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Genomics, v.15, n. 1, p. 535, Jun. 2014. |
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DOI: |
10.1186/1471-2164-15-535 |
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Idioma: |
Português |
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Notas: |
The complete genome of Burkholderia phenoliruptrix strain BR3459a, a symbiont of Mimosa flocculosa: highlighting the coexistence of symbiotic and pathogenic genes |
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Conteúdo: |
BACKGROUND: Burkholderia species play an important ecological role related to xenobiosis, the promotion of plant growth, the biocontrol of agricultural diseases, and symbiotic and non-symbiotic biological nitrogen fixation. Here, we highlight our study as providing the first complete genome of a symbiotic strain of B. phenoliruptrix, BR3459a (=CLA1), which was originally isolated in Brazil from nodules of Mimosa flocculosa and is effective in fixing nitrogen in association with this leguminous species. RESULTS: Genomic comparisons with other pathogenic and non-pathogenic Burkholderia strains grouped B. phenoliruptrix BR3459a with plant-associated beneficial and environmental species, although it shares a high percentage of its gene repertoire with species of the B. cepacia complex (Bcc) and "pseudomallei" group. The genomic analyses showed that the bce genes involved in exopolysaccharide production are clustered together in the same genomic region, constituting part of the Group III cluster of non-pathogenic bacteria. Regarding environmental stresses, we highlight genes that might be relevant in responses to osmotic, heat, cold and general stresses. Furthermore, a number of particularly interesting genes involved in the machinery of the T1SS, T2SS, T3SS, T4ASS and T6SS secretion systems were identified. The xenobiotic properties of strain BR3459a were also investigated, and some enzymes involved in the degradation of styrene, nitrotoluene, dioxin, chlorocyclohexane, chlorobenzene and caprolactam were identified. The genomic analyses also revealed a large number of antibiotic-related genes, the most important of which were correlated with streptomycin and novobiocin. The symbiotic plasmid showed high sequence identity with the symbiotic plasmid of B. phymatum. Additionally, comparative analysis of 545 housekeeping genes among pathogenic and non-pathogenic Burkholderia species strongly supports the definition of a new genus for the second branch, which would include BR3459a. CONCLUSIONS: The analyses of B. phenoliruptrix BR3459a showed key property of fixing nitrogen that together with genes for high tolerance to environmental stresses might explain a successful strategy of symbiosis in the tropics. The strain also harbours interesting sets of genes with biotechnological potential. However, the resemblance of certain genes to those of pathogenic Burkholderia raise concerns about large-scale applications in agriculture or for bioremediation. MenosBACKGROUND: Burkholderia species play an important ecological role related to xenobiosis, the promotion of plant growth, the biocontrol of agricultural diseases, and symbiotic and non-symbiotic biological nitrogen fixation. Here, we highlight our study as providing the first complete genome of a symbiotic strain of B. phenoliruptrix, BR3459a (=CLA1), which was originally isolated in Brazil from nodules of Mimosa flocculosa and is effective in fixing nitrogen in association with this leguminous species. RESULTS: Genomic comparisons with other pathogenic and non-pathogenic Burkholderia strains grouped B. phenoliruptrix BR3459a with plant-associated beneficial and environmental species, although it shares a high percentage of its gene repertoire with species of the B. cepacia complex (Bcc) and "pseudomallei" group. The genomic analyses showed that the bce genes involved in exopolysaccharide production are clustered together in the same genomic region, constituting part of the Group III cluster of non-pathogenic bacteria. Regarding environmental stresses, we highlight genes that might be relevant in responses to osmotic, heat, cold and general stresses. Furthermore, a number of particularly interesting genes involved in the machinery of the T1SS, T2SS, T3SS, T4ASS and T6SS secretion systems were identified. The xenobiotic properties of strain BR3459a were also investigated, and some enzymes involved in the degradation of styrene, nitrotoluene, dioxin, chlorocyclohexane, chlorobe... Mostrar Tudo |
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Palavras-Chave: |
Comparative genomics; FBN; Genômica; Nitrogen fixing; Pathogenic Bacteria. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03613naa a2200325 a 4500
001 1997358
005 2015-01-23
008 2014 bl --- 0-- u #d
024 7 $a10.1186/1471-2164-15-535$2DOI
100 1 $aZULETA, L. F. G.
245 $aThe complete genome of Burkholderia phenoliruptrix strain BR3459a, a symbiont of Mimosa flocculosa$bhighlighting the coexistence of symbiotic and pathogenic genes.
260 $c2014
500 $aThe complete genome of Burkholderia phenoliruptrix strain BR3459a, a symbiont of Mimosa flocculosa: highlighting the coexistence of symbiotic and pathogenic genes
520 $aBACKGROUND: Burkholderia species play an important ecological role related to xenobiosis, the promotion of plant growth, the biocontrol of agricultural diseases, and symbiotic and non-symbiotic biological nitrogen fixation. Here, we highlight our study as providing the first complete genome of a symbiotic strain of B. phenoliruptrix, BR3459a (=CLA1), which was originally isolated in Brazil from nodules of Mimosa flocculosa and is effective in fixing nitrogen in association with this leguminous species. RESULTS: Genomic comparisons with other pathogenic and non-pathogenic Burkholderia strains grouped B. phenoliruptrix BR3459a with plant-associated beneficial and environmental species, although it shares a high percentage of its gene repertoire with species of the B. cepacia complex (Bcc) and "pseudomallei" group. The genomic analyses showed that the bce genes involved in exopolysaccharide production are clustered together in the same genomic region, constituting part of the Group III cluster of non-pathogenic bacteria. Regarding environmental stresses, we highlight genes that might be relevant in responses to osmotic, heat, cold and general stresses. Furthermore, a number of particularly interesting genes involved in the machinery of the T1SS, T2SS, T3SS, T4ASS and T6SS secretion systems were identified. The xenobiotic properties of strain BR3459a were also investigated, and some enzymes involved in the degradation of styrene, nitrotoluene, dioxin, chlorocyclohexane, chlorobenzene and caprolactam were identified. The genomic analyses also revealed a large number of antibiotic-related genes, the most important of which were correlated with streptomycin and novobiocin. The symbiotic plasmid showed high sequence identity with the symbiotic plasmid of B. phymatum. Additionally, comparative analysis of 545 housekeeping genes among pathogenic and non-pathogenic Burkholderia species strongly supports the definition of a new genus for the second branch, which would include BR3459a. CONCLUSIONS: The analyses of B. phenoliruptrix BR3459a showed key property of fixing nitrogen that together with genes for high tolerance to environmental stresses might explain a successful strategy of symbiosis in the tropics. The strain also harbours interesting sets of genes with biotechnological potential. However, the resemblance of certain genes to those of pathogenic Burkholderia raise concerns about large-scale applications in agriculture or for bioremediation.
653 $aComparative genomics
653 $aFBN
653 $aGenômica
653 $aNitrogen fixing
653 $aPathogenic Bacteria
700 1 $aCUNHA, C. de O.
700 1 $aCARVALHO, F. M. de
700 1 $aCIAPINA, L. P.
700 1 $aSOUZA, R. C
700 1 $aMERCANTE, F. M.
700 1 $aFARIA, S. M. de
700 1 $aBALDANI, J. I.
700 1 $aSTRALIOTTO, R.
700 1 $aHUNGRIA, M.
700 1 $aVASCONCELOS, A. T. R. de
773 $tBMC Genomics$gv.15, n. 1, p. 535, Jun. 2014.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 27 | |
| 8. |  | CARVALHO, F. M. de; VIANA, A. E. S.; MATSUMATO, S. N.; CARDOSO, C. E. L.; GOMES, I. R. Aspectos sociofundiários do cultivo da mandioca em treze municípios da região sudoeste da Bahia. In: CONGRESSO BRASILEIRO DA MANDIOCA, 12., 2007, Paranavaí. Mandioca: bioenergia, alimento e renda. Botucatu: CERAT- UNESP, [2007].| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 9. | | COSTA, J. L.; OLIVEIRA, E. J. de; OLIVEIRA, G. A. F.; CARVALHO, F. M. de; DANTAS, J. L. L. Otimização de locos de microssatélites para diferentes espécies de Passiflora. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 4., 2010, Cruz das Almas. [Anais...] Cruz das Almas: Embrapa Mandioca e Fruticultura, 2010. (Embrapa Mandioca e Fruticultura. Documentos, 190). 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 10. | | CARVALHO, F. M. de; VIANA, A. E. S.; CARDOSO, C. E. L.; MATSUMOTO, S. N.; GOMES, I. R. Sistemas de produção de mandioca em treze municípios da Região Sudoeste da Bahia. Bragantia, Campinas, v. 68, n. 3, p. 699-702, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 11. | | FONSECA, N.; REIS, R. V. dos; SANTOS, L. F. dos; SILVA, P. H. da; CARVALHO, F. M. de; MAGALHÃES, V. C.; DALTRO, P. S. Caracterização e estimação de parâmetros genéticos em genótipos de mangueira. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 5., 2009, Guarapari. O melhoramento e os novos cenários da agricultura: anais. Vitória: Incaper, 2009. 1 CD-ROM. pdf 2822| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 12. | | OLIVEIRA, G. A. F.; OLIVEIRA, E. J. de; CARVALHO, F. M. de C.; SILVA, A. dos S.; COSTA, J. L; DANTAS, J. L. L. Identificação de genótipos com alto nível de homozigosidade visando à obtenção de linhagens de mamoeiro por seleção assistida por marcadores microssatélites. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 4., 2010, Cruz das Almas. [Anais...] Cruz das Almas: Embrapa Mandioca e Fruticultura, 2010. (Embrapa Mandioca e Fruticultura. Documentos, 190).1 CD-ROM. PDF. 014.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 13. | | SILVA, A. dos S.; OLIVEIRA, E. J. de; CARVALHO, F. M. de; SANTOS, L. F. dos; COSTA, J. L.; AMORIM, V. B. de O. Identificação de linhagens de mamoeiro por meio da seleção assistida por marcadores microssatélites. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA TROPICAL, 3., 2009, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | SILVA, A. dos S.; OLIVEIRA, E. J. de; CARVALHO, F. M. de; SANTOS, L. F. dos; AMORIM, V. B. de O.; DANTAS, J. L. L. Identificação de linhagens de mamoeiro por seleção assistida por marcadores microssatélites. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 5., 2009, Guarapari. O melhoramento e os novos cenários da agricultura: anais. Vitória: Incaper, 2009. 1 CD-ROM. pdf 2508| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 15. | | SILVA, A. dos S.; OLIVEIRA, E. J. de; CARVALHO, F. M. de; SANTOS, L. F. dos; COSTA, J. L.; AMORIM, V. B. de O. Identificação de linhagens de mamoeiro por seleção assistida por marcadores microssatélites. In: SEMINÁRIO DE PESQUISA DO RECÔNCAVO DA BAHIA, 3.; SEMINÁRIO ESTUDANTIL DE PESQUISA DA UFRB, 3.; SEMINÁRIO DE PÓS-GRADUAÇÃO DA UFRB, 3., 2009, Cruz das Almas. Evolução do conhecimento e o desenvolvimento social: [resumos...]. Cruz das Almas: Universidade Federal do Recôncavo da Bahia, 2009. 01 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 16. | | SANTOS, L. F. dos; OLIVEIRA, E. J. de; SILVA, A. dos S.; CARVALHO, F. M. de; COSTA, J. l.; PADUA, J. G. ISSR Markers as a Tool for the Assessment of Genetic Diversity in Passiflora Biochemical Genetics, v.49, n.7-8, p.540-554, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | OLIVEIRA, E. J. de; SILVA, A. dos S.; CARVALHO, F. M. de; SANTOS, L. F. dos; COSTA, J. L.; AMORIM, V. B. de O.; DANTAS, J. L. L. Polymorphic microsatellite marker set for Carica papaya L. and its use in molecular-assisted selection. Euphytica, Wageningen, v. 173, n. 2, p. 279-287, 2010. Disponivel em: Acesso em: 14 out. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 18. | | OLIVEIRA, E. J. de; COSTA, J. L.; SANTOS, L. F. dos; CARVALHO, F. M. de; SILVA, A. dos S.; DANTAS, J. L. L. Molecular characterization of papaya genotypes using AFLP markers. Revista Brasileira de Fruticultura, Jaboticabal, v. 33, n. 3, p. 849-858, set, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 19. | | CARVALHO, F. M. de; VIANA, A. E. S.; MATSUMOTO, S. N.; REBOUÇAS, T. N. H.; CARDOSO, C. E. L.; GOMES, I. R. Manejo de solo em cultvo com mandioca em treze municípios da região sudoeste da Bahia. Ciência e Agrotecnologia, Lavras, v. 31, n. 2, p. 378-384, mar/abr, 2007| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 27 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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