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| Registros recuperados : 506 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
10/03/2016 |
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Data da última atualização: |
10/03/2016 |
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Autoria: |
SANTOS, F. C.; SILVA, J. F.; BOELONI, J. N.; TEIXEIRA, E.; TURRA, E. M.; SERAKIDES, R.; OCARINO, N. M. |
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Afiliação: |
FERNANDA C. SANTOS, EV/UFMG; JUNEO F. SILVA, EV/UFMG; JANKERLE N. BOELONI, UV/UFMG; EDGAR TEIXEIRA, EV/UFMG; EDUARDO M. TURRA, UV/UFMG; ROGÉRIA SERAKIDES, EV/UFMG; NATÁLIA M. OCARINO, EV/UFMG. |
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Título: |
Morphological and immunohistochemical characterization of angiogenic and apoptotic factors and the expression of thyroid receptors in the ovary of tilapia Oreochromis niloticus in captivity. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35. n. 4, p. 371-376, abr. 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Morphological and immunohistochemical characterization of angiogenic and apoptotic factors and the expression of thyroid receptors in the ovary of tilapia Oreochromis niloticus in captivity were studied. The morphological evaluation of the ovaries was performed by histological paraffin embedded and stained with HE. The immunohistochemical expressions of CDC47, VEGF, Flk-1, angiopoietin, Tie-2 and thyroid receptor (TRα) were performed by the technique of streptavidein-biotin-peroxidase. Apoptosis was assessed using the TUNEL kit. The relative expression of thyroid hormone receptors (TRα and TRβ) was assessed by RT-PCR real time. The nuclear expression of CDC47 increased with the stage of maturation of the oocyte and was observed in the follicle cells. Apoptotic bodies were observed in the follicular cells of atretic follicles and postovulatory follicles from the ovaries of 150g and 350g fish. Expression of VEGF and its receptor Flk-1 was also observed in the follicular cells, and the expression of both increased with the maturity of the oocyte, with a higher intensity observed in the full-grown follicle. The expression of angiopoietin and of its receptor (Tie 2) was discrete and moderate respectively. TRα expression was independent of follicular development. However, the 350 g tilapia exhibited higher expression of TRβ compared with the 50 g tilapia. We conclude that the proliferative activity and the expression of VEGF and its receptor increase with follicular maturation and that the TRs expression increases with ovarian maturity in tilapia (Oreochromis niloticus). MenosMorphological and immunohistochemical characterization of angiogenic and apoptotic factors and the expression of thyroid receptors in the ovary of tilapia Oreochromis niloticus in captivity were studied. The morphological evaluation of the ovaries was performed by histological paraffin embedded and stained with HE. The immunohistochemical expressions of CDC47, VEGF, Flk-1, angiopoietin, Tie-2 and thyroid receptor (TRα) were performed by the technique of streptavidein-biotin-peroxidase. Apoptosis was assessed using the TUNEL kit. The relative expression of thyroid hormone receptors (TRα and TRβ) was assessed by RT-PCR real time. The nuclear expression of CDC47 increased with the stage of maturation of the oocyte and was observed in the follicle cells. Apoptotic bodies were observed in the follicular cells of atretic follicles and postovulatory follicles from the ovaries of 150g and 350g fish. Expression of VEGF and its receptor Flk-1 was also observed in the follicular cells, and the expression of both increased with the maturity of the oocyte, with a higher intensity observed in the full-grown follicle. The expression of angiopoietin and of its receptor (Tie 2) was discrete and moderate respectively. TRα expression was independent of follicular development. However, the 350 g tilapia exhibited higher expression of TRβ compared with the 50 g tilapia. We conclude that the proliferative activity and the expression of VEGF and its receptor increase with ... Mostrar Tudo |
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Palavras-Chave: |
Angiogenic factor; Apoptotic factor; Fator angiogênico; Fator apoptótico; Imuno-histoquímica; Receptor tireoidiano; Thyroid receptors. |
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Thesagro: |
Oreochromis Niloticus; Ovário; Peixe; Tilápia. |
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Thesaurus NAL: |
Fish; Immunohistochemistry. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140987/1/Morphological-and-immunohistochemical.pdf
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Marc: |
LEADER 02703naa a2200349 a 4500
001 2040532
005 2016-03-10
008 2015 bl uuuu u00u1 u #d
100 1 $aSANTOS, F. C.
245 $aMorphological and immunohistochemical characterization of angiogenic and apoptotic factors and the expression of thyroid receptors in the ovary of tilapia Oreochromis niloticus in captivity.
260 $c2015
520 $aMorphological and immunohistochemical characterization of angiogenic and apoptotic factors and the expression of thyroid receptors in the ovary of tilapia Oreochromis niloticus in captivity were studied. The morphological evaluation of the ovaries was performed by histological paraffin embedded and stained with HE. The immunohistochemical expressions of CDC47, VEGF, Flk-1, angiopoietin, Tie-2 and thyroid receptor (TRα) were performed by the technique of streptavidein-biotin-peroxidase. Apoptosis was assessed using the TUNEL kit. The relative expression of thyroid hormone receptors (TRα and TRβ) was assessed by RT-PCR real time. The nuclear expression of CDC47 increased with the stage of maturation of the oocyte and was observed in the follicle cells. Apoptotic bodies were observed in the follicular cells of atretic follicles and postovulatory follicles from the ovaries of 150g and 350g fish. Expression of VEGF and its receptor Flk-1 was also observed in the follicular cells, and the expression of both increased with the maturity of the oocyte, with a higher intensity observed in the full-grown follicle. The expression of angiopoietin and of its receptor (Tie 2) was discrete and moderate respectively. TRα expression was independent of follicular development. However, the 350 g tilapia exhibited higher expression of TRβ compared with the 50 g tilapia. We conclude that the proliferative activity and the expression of VEGF and its receptor increase with follicular maturation and that the TRs expression increases with ovarian maturity in tilapia (Oreochromis niloticus).
650 $aFish
650 $aImmunohistochemistry
650 $aOreochromis Niloticus
650 $aOvário
650 $aPeixe
650 $aTilápia
653 $aAngiogenic factor
653 $aApoptotic factor
653 $aFator angiogênico
653 $aFator apoptótico
653 $aImuno-histoquímica
653 $aReceptor tireoidiano
653 $aThyroid receptors
700 1 $aSILVA, J. F.
700 1 $aBOELONI, J. N.
700 1 $aTEIXEIRA, E.
700 1 $aTURRA, E. M.
700 1 $aSERAKIDES, R.
700 1 $aOCARINO, N. M.
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35. n. 4, p. 371-376, abr. 2015.
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Embrapa Unidades Centrais (AI-SEDE) |
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