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Registro Completo |
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
15/02/2017 |
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Data da última atualização: |
15/02/2017 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CARVALHO, A. J. de; SOUZA, V. B. de; SILVA, K. J. D. e; ROCHA, M. de M.; PEREIRA FILHO, I. A.; BATISTA, P. S. C.; BRITO, O. G. |
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Afiliação: |
ABNER JOSE DE CARVALHO, UNIMONTES, Campos de Janaúba, MG.; VANET BATISTA DE SOUZA, UEM, Colombo, PR.; KAESEL JACKSON DAMASCENO E SILVA, CPAMN; MAURISRAEL DE MOURA ROCHA, CPAMN; ISRAEL ALEXANDRE PEREIRA FILHO, CNPMS; PAULO SERGIO CARDOSO BATISTA, UNIMONTES, Campos de Janaúba, MG.; ORLANDO GONÇALVES BRITO, UFVJM, Diamantina, MG. |
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Título: |
Adaptabilidade e estabilidade produtiva de linhagens elite e cultivares de feijão-caupi de porte prostrado em ambientes de Mato Grosso e Minas Gerais. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 4., 2016, Sorriso. Feijão-caupi: avanços e desafios tecnológicos e de mercado: resumos. Brasília, DF: Embrapa, 2016. |
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Páginas: |
p. 131. |
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Idioma: |
Português |
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Notas: |
Na publicação: KAESEL JACKSON DAMASCENO-SILVA |
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Palavras-Chave: |
Ensaios de VCU. |
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Thesagro: |
Feijão de corda; Produtividade; Vigna unguiculata. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/155027/1/Adaptabilidade-estabilidade-21.pdf
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Marc: |
LEADER 00961naa a2200253 a 4500
001 2064324
005 2017-02-15
008 2016 bl uuuu u00u1 u #d
100 1 $aCARVALHO, A. J. de
245 $aAdaptabilidade e estabilidade produtiva de linhagens elite e cultivares de feijão-caupi de porte prostrado em ambientes de Mato Grosso e Minas Gerais.$h[electronic resource]
260 $c2016
300 $ap. 131.
500 $aNa publicação: KAESEL JACKSON DAMASCENO-SILVA
650 $aFeijão de corda
650 $aProdutividade
650 $aVigna unguiculata
653 $aEnsaios de VCU
700 1 $aSOUZA, V. B. de
700 1 $aSILVA, K. J. D. e
700 1 $aROCHA, M. de M.
700 1 $aPEREIRA FILHO, I. A.
700 1 $aBATISTA, P. S. C.
700 1 $aBRITO, O. G.
773 $tIn: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 4., 2016, Sorriso. Feijão-caupi: avanços e desafios tecnológicos e de mercado: resumos. Brasília, DF: Embrapa, 2016.
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
13/10/2020 |
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Data da última atualização: |
19/03/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
TEIXEIRA, N. C.; WENDLAND, A.; OLIVEIRA, M. I. de S.; BRANDÃO, L. T. D.; SOUZA, T. L. P. O. de; FARIA, J. C. de; DEL PELOSO, M. J.; CORTES, M. V. de C. B. |
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Afiliação: |
NARA CRISTINA TEIXEIRA, bolsista CNPAF; ADRIANE WENDLAND FERREIRA, CNPAF; MAYTHSULENE INACIO DE SOUZA OLIVEIRA, bolsista CNPAF; LIVIA TEIXEIRA DUARTE BRANDAO, CNPAF; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; JOSIAS CORREA DE FARIA, CNPAF; MARIA JOSE DEL PELOSO, CNPAF; MARCIO VINICIUS DE C BARROS CORTES, CNPAF. |
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Título: |
Rapid identification of RNA-interference-based resistance to Bean golden mosaic virus in transgenic common beans via loop-mediated isothermal amplification. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Crop Science, v. 60, n. 6, p. 3004-3012, Nov./Dec. 2020. |
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ISSN: |
1435-0653 |
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DOI: |
https://doi.org/10.1002/csc2.20107 |
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Idioma: |
Inglês |
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Conteúdo: |
Amolecular toolwas adapted for practical identification of theworld?s first transgenic event (Embrapa 5.1) in common bean (Phaseolus vulgaris L.). Based on the technology of RNA interference and on the transformation of plants via the Biobalistic method, a transgenic bean was created with effective resistance to Bean golden mosaic virus, its principal virus. To support the monitoring of this technology, the development of new cultivars, and the process of producing, benefiting, and distributing of seeds and grains, a molecular detection tool based on loop-mediated isothermal amplification (LAMP) was developed. This tool can facilitating diagnosis, making it fast, specific, low-cost, and easily executed. Based on the gene Ahas (GenBank M88686) of the Embrapa 5.1 event, sets of initiators were planned (forward outer primer?backward outer primer and forward inner primer?backward inner primer) that are characteristic of the LAMP method. These were evaluated for their sensitivity and specificity in detecting their target. The LAMP method allows direct visual interpretation. The Neutral Red (NR) pH indicator was adopted to facilitate molecular diagnosis in moderately well equipped environments, thus reducing the risk of contamination and the need for skilled labor. This agility in diagnosis occurs because several phases commonly used inmolecular techniques, such as polymerase chain reaction, can be excluded. The LAMPmethod, combined with a rapid DNA extraction method (modified NaOH) and the addition of NR, provides technological support for addressing issues of biosafety, traceability, and identification of the Embrapa 5.1 event in beans in the field and in industry. MenosAmolecular toolwas adapted for practical identification of theworld?s first transgenic event (Embrapa 5.1) in common bean (Phaseolus vulgaris L.). Based on the technology of RNA interference and on the transformation of plants via the Biobalistic method, a transgenic bean was created with effective resistance to Bean golden mosaic virus, its principal virus. To support the monitoring of this technology, the development of new cultivars, and the process of producing, benefiting, and distributing of seeds and grains, a molecular detection tool based on loop-mediated isothermal amplification (LAMP) was developed. This tool can facilitating diagnosis, making it fast, specific, low-cost, and easily executed. Based on the gene Ahas (GenBank M88686) of the Embrapa 5.1 event, sets of initiators were planned (forward outer primer?backward outer primer and forward inner primer?backward inner primer) that are characteristic of the LAMP method. These were evaluated for their sensitivity and specificity in detecting their target. The LAMP method allows direct visual interpretation. The Neutral Red (NR) pH indicator was adopted to facilitate molecular diagnosis in moderately well equipped environments, thus reducing the risk of contamination and the need for skilled labor. This agility in diagnosis occurs because several phases commonly used inmolecular techniques, such as polymerase chain reaction, can be excluded. The LAMPmethod, combined with a rapid DNA extraction method (modified NaO... Mostrar Tudo |
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Thesagro: |
DNA; Feijão; Mosaico Dourado; Phaseolus Vulgaris; Planta Transgênica. |
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Thesaurus NAL: |
Bean golden mosaic virus; Beans; Loop-mediated isothermal amplification; RNA interference; Transgenic plants. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02809naa a2200349 a 4500
001 2125441
005 2021-03-19
008 2020 bl uuuu u00u1 u #d
022 $a1435-0653
024 7 $ahttps://doi.org/10.1002/csc2.20107$2DOI
100 1 $aTEIXEIRA, N. C.
245 $aRapid identification of RNA-interference-based resistance to Bean golden mosaic virus in transgenic common beans via loop-mediated isothermal amplification.$h[electronic resource]
260 $c2020
520 $aAmolecular toolwas adapted for practical identification of theworld?s first transgenic event (Embrapa 5.1) in common bean (Phaseolus vulgaris L.). Based on the technology of RNA interference and on the transformation of plants via the Biobalistic method, a transgenic bean was created with effective resistance to Bean golden mosaic virus, its principal virus. To support the monitoring of this technology, the development of new cultivars, and the process of producing, benefiting, and distributing of seeds and grains, a molecular detection tool based on loop-mediated isothermal amplification (LAMP) was developed. This tool can facilitating diagnosis, making it fast, specific, low-cost, and easily executed. Based on the gene Ahas (GenBank M88686) of the Embrapa 5.1 event, sets of initiators were planned (forward outer primer?backward outer primer and forward inner primer?backward inner primer) that are characteristic of the LAMP method. These were evaluated for their sensitivity and specificity in detecting their target. The LAMP method allows direct visual interpretation. The Neutral Red (NR) pH indicator was adopted to facilitate molecular diagnosis in moderately well equipped environments, thus reducing the risk of contamination and the need for skilled labor. This agility in diagnosis occurs because several phases commonly used inmolecular techniques, such as polymerase chain reaction, can be excluded. The LAMPmethod, combined with a rapid DNA extraction method (modified NaOH) and the addition of NR, provides technological support for addressing issues of biosafety, traceability, and identification of the Embrapa 5.1 event in beans in the field and in industry.
650 $aBean golden mosaic virus
650 $aBeans
650 $aLoop-mediated isothermal amplification
650 $aRNA interference
650 $aTransgenic plants
650 $aDNA
650 $aFeijão
650 $aMosaico Dourado
650 $aPhaseolus Vulgaris
650 $aPlanta Transgênica
700 1 $aWENDLAND, A.
700 1 $aOLIVEIRA, M. I. de S.
700 1 $aBRANDÃO, L. T. D.
700 1 $aSOUZA, T. L. P. O. de
700 1 $aFARIA, J. C. de
700 1 $aDEL PELOSO, M. J.
700 1 $aCORTES, M. V. de C. B.
773 $tCrop Science$gv. 60, n. 6, p. 3004-3012, Nov./Dec. 2020.
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Embrapa Arroz e Feijão (CNPAF) |
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