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Registro Completo |
Biblioteca(s): |
Embrapa Soja; Embrapa Trigo. |
Data corrente: |
27/09/2023 |
Data da última atualização: |
27/09/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA. D. A. da; CARPENTIERI-PIPOLO, V.; BARRETO, T.; ABDELNOOR, R. V.; MARIN, S. R. R.; CARRAO-PANIZZI, M. C. |
Afiliação: |
Instituto Agronômico Campinas; VALERIA CARPENTIERI PIPOLO, CNPT; Syngenta Proteção de Cultivos LTDA; RICARDO VILELA ABDELNOOR, CNPSO; SILVANA REGINA ROCKENBACH MARIN, CNPSO; MERCEDES CONCÓRDIA CARRÃO-PANIZZI. |
Título: |
Genetic removal of trypsin inhibitor and lipoxygenase isozymes form soybean seeds (Glycine max) by simple sequence repeat marker assisted selection. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 22, n. 3, GMR19145, 2023. |
DOI: |
https://doi.org/10.4238/gmr19145 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: unitz trypsin inhibitor (KTI), which affects protein digestibility and lipoxygenase isozymes, responsible for the off-flavor associated with soy-based foods, are two undesirable factors present in soybean seeds. These unpleasant factors are usually inactivated by heat treatment. However, heat treatment does not completely eliminate these factors; in addition it may decrease protein solubility and may incur extra energy costs. Genetic elimination of these factors could be an alternative to heat treatment. This study aimed to select soybean lines free of KTI and lipoxygenase isozymes in the seeds. The population under study was obtained by crossing the BRS 213 cultivar, which shows low lipoxygenase activity, with BRS 155, a KTI lacking cultivar. F2:3 hybrid populations were selected and analyzed using DNA markers for the identification of recessive alleles that encode the absence of KTI and the three lipoxygenase enzymes (LOX1, LOX2 and LOX3). F2:3 segregating populations were successfully identified with the KTI specific marker with 100% efficiency. However, the KTi/kti-gene-specific marker does not allow for the identification of heterozygous and homozygous genotypes with dominant KTi alleles; the the simple sequence repeat (SSR) markers Satt228 and Satt409, which bind tightly to the KTi locus, were much more suitable diagnostic marker for screening plants in segregating populations. Satt090 and Satt417 confirmed the presence of the homozygous Lx2 null-allele in the parental cultivar BRS 213 by flanking Lx2 loci at 3.00 and 2.77 cM, respectively. The SSR markers used in this study could be efficiently used in marker assisted selection in a breeding program aimed at improving soybean seed quality. MenosAbstract: unitz trypsin inhibitor (KTI), which affects protein digestibility and lipoxygenase isozymes, responsible for the off-flavor associated with soy-based foods, are two undesirable factors present in soybean seeds. These unpleasant factors are usually inactivated by heat treatment. However, heat treatment does not completely eliminate these factors; in addition it may decrease protein solubility and may incur extra energy costs. Genetic elimination of these factors could be an alternative to heat treatment. This study aimed to select soybean lines free of KTI and lipoxygenase isozymes in the seeds. The population under study was obtained by crossing the BRS 213 cultivar, which shows low lipoxygenase activity, with BRS 155, a KTI lacking cultivar. F2:3 hybrid populations were selected and analyzed using DNA markers for the identification of recessive alleles that encode the absence of KTI and the three lipoxygenase enzymes (LOX1, LOX2 and LOX3). F2:3 segregating populations were successfully identified with the KTI specific marker with 100% efficiency. However, the KTi/kti-gene-specific marker does not allow for the identification of heterozygous and homozygous genotypes with dominant KTi alleles; the the simple sequence repeat (SSR) markers Satt228 and Satt409, which bind tightly to the KTi locus, were much more suitable diagnostic marker for screening plants in segregating populations. Satt090 and Satt417 confirmed the presence of the homozygous Lx2 null-allele in th... Mostrar Tudo |
Palavras-Chave: |
Inibidor de tripsina; Kunitz Isoenzimas lipoxigenase; Kunitz trypsin inhibitor; Lipoxygenase isozymes; Molecular markers. |
Thesagro: |
Controle de Qualidade; Glycine Max; Marcador Molecular; Semente; Soja. |
Thesaurus Nal: |
Seeds; Soybeans. |
Categoria do assunto: |
-- X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156934/1/genetic-removal-trypsin-inhibitor-and-lipoxygenase-isozymes-form-soybean-seeds-glycine-max.pdf
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Marc: |
LEADER 02807naa a2200337 a 4500 001 2156934 005 2023-09-27 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.4238/gmr19145$2DOI 100 1 $aSILVA. D. A. da 245 $aGenetic removal of trypsin inhibitor and lipoxygenase isozymes form soybean seeds (Glycine max) by simple sequence repeat marker assisted selection.$h[electronic resource] 260 $c2023 520 $aAbstract: unitz trypsin inhibitor (KTI), which affects protein digestibility and lipoxygenase isozymes, responsible for the off-flavor associated with soy-based foods, are two undesirable factors present in soybean seeds. These unpleasant factors are usually inactivated by heat treatment. However, heat treatment does not completely eliminate these factors; in addition it may decrease protein solubility and may incur extra energy costs. Genetic elimination of these factors could be an alternative to heat treatment. This study aimed to select soybean lines free of KTI and lipoxygenase isozymes in the seeds. The population under study was obtained by crossing the BRS 213 cultivar, which shows low lipoxygenase activity, with BRS 155, a KTI lacking cultivar. F2:3 hybrid populations were selected and analyzed using DNA markers for the identification of recessive alleles that encode the absence of KTI and the three lipoxygenase enzymes (LOX1, LOX2 and LOX3). F2:3 segregating populations were successfully identified with the KTI specific marker with 100% efficiency. However, the KTi/kti-gene-specific marker does not allow for the identification of heterozygous and homozygous genotypes with dominant KTi alleles; the the simple sequence repeat (SSR) markers Satt228 and Satt409, which bind tightly to the KTi locus, were much more suitable diagnostic marker for screening plants in segregating populations. Satt090 and Satt417 confirmed the presence of the homozygous Lx2 null-allele in the parental cultivar BRS 213 by flanking Lx2 loci at 3.00 and 2.77 cM, respectively. The SSR markers used in this study could be efficiently used in marker assisted selection in a breeding program aimed at improving soybean seed quality. 650 $aSeeds 650 $aSoybeans 650 $aControle de Qualidade 650 $aGlycine Max 650 $aMarcador Molecular 650 $aSemente 650 $aSoja 653 $aInibidor de tripsina 653 $aKunitz Isoenzimas lipoxigenase 653 $aKunitz trypsin inhibitor 653 $aLipoxygenase isozymes 653 $aMolecular markers 700 1 $aCARPENTIERI-PIPOLO, V. 700 1 $aBARRETO, T. 700 1 $aABDELNOOR, R. V. 700 1 $aMARIN, S. R. R. 700 1 $aCARRAO-PANIZZI, M. C. 773 $tGenetics and Molecular Research$gv. 22, n. 3, GMR19145, 2023.
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Embrapa Trigo (CNPT) |
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Registro Completo
Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
22/03/1996 |
Data da última atualização: |
22/03/1996 |
Autoria: |
POTT, A.; POTT, V. J.; CATELLA, A. C.; SALIS, S. M. de; PEREIRA, M.; CAMPOS, Z. M. da S.; MOURAO, G. de M.; MATTOS, P. P. de; PINHEIRO, M. S.; SILVA, R. A. e. |
Afiliação: |
EMBRAPA. Centro de Pesquisa Agropecuaria do Pantanal (Corumba, MS). |
Título: |
Chave de identificacao via vegetativa de plantas lenhosas da fazenda Nhumirim, Nhecolandia, Pantanal. |
Ano de publicação: |
1988 |
Fonte/Imprenta: |
In: CONGRESSO NACIONAL BOTANICA, 39., Belem, 1988. Resumos... Belem: Sociedade Botanica do Brasil, 1988. v.1, p.53. |
Idioma: |
Português |
Conteúdo: |
Foi elaborada uma chave dicotomica para identificacao, atraves de caracteristicas vegetativas, de 170 arvores e arbustos da Reserva Biologica, uma area de 600 ha, na fazenda Nhumirim (lat. 18o59'S, long. 56o39'W), do Centro de Pesquisa Agropecuaria do Pantanal/EMBRAPA, na Nhecolandia, municipio de Corumba, MS, no Pantanal arenoso. Dados sobre porte, ritidoma, presenca de latex, espinhos e nectarios extraflorais, folha (filotaxia, tipo, forma, bordos, nervacao, dimensoes, coloracao, indumento, odor, etc.) foram anotadas no campo, para cada especie. Foram percorridos dos diversos ambientes: campo, caronal (Elyonuretum), caapao, cerradao e mata semidecicua. A chave teve como primeiro resultado a familiarizacao da equipe com as plantas e devera servir para futurosprojetos na area de ecologia e recursos naturais. |
Palavras-Chave: |
Identification; Plant. |
Thesagro: |
Flora; Identificação; Planta; Vegetação. |
Thesaurus NAL: |
Pantanal; vegetation. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01750naa a2200325 a 4500 001 1787898 005 1996-03-22 008 1988 bl --- 0-- u #d 100 1 $aPOTT, A. 245 $aChave de identificacao via vegetativa de plantas lenhosas da fazenda Nhumirim, Nhecolandia, Pantanal. 260 $c1988 520 $aFoi elaborada uma chave dicotomica para identificacao, atraves de caracteristicas vegetativas, de 170 arvores e arbustos da Reserva Biologica, uma area de 600 ha, na fazenda Nhumirim (lat. 18o59'S, long. 56o39'W), do Centro de Pesquisa Agropecuaria do Pantanal/EMBRAPA, na Nhecolandia, municipio de Corumba, MS, no Pantanal arenoso. Dados sobre porte, ritidoma, presenca de latex, espinhos e nectarios extraflorais, folha (filotaxia, tipo, forma, bordos, nervacao, dimensoes, coloracao, indumento, odor, etc.) foram anotadas no campo, para cada especie. Foram percorridos dos diversos ambientes: campo, caronal (Elyonuretum), caapao, cerradao e mata semidecicua. A chave teve como primeiro resultado a familiarizacao da equipe com as plantas e devera servir para futurosprojetos na area de ecologia e recursos naturais. 650 $aPantanal 650 $avegetation 650 $aFlora 650 $aIdentificação 650 $aPlanta 650 $aVegetação 653 $aIdentification 653 $aPlant 700 1 $aPOTT, V. J. 700 1 $aCATELLA, A. C. 700 1 $aSALIS, S. M. de 700 1 $aPEREIRA, M. 700 1 $aCAMPOS, Z. M. da S. 700 1 $aMOURAO, G. de M. 700 1 $aMATTOS, P. P. de 700 1 $aPINHEIRO, M. S. 700 1 $aSILVA, R. A. e. 773 $tIn: CONGRESSO NACIONAL BOTANICA, 39., Belem, 1988. Resumos... Belem: Sociedade Botanica do Brasil, 1988.$gv.1, p.53.
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