| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Trigo. |
|
Data corrente: |
06/12/2021 |
|
Data da última atualização: |
06/12/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
DIMKIĆ, I.; BHARDWAJ, V.; CARPENTIERI-PIPOLO, V.; KUZMANOVIĆ, N.; DEGRASSI, G. |
|
Afiliação: |
IVICA DIMKIĆ, Department of Biochemistry and Molecular Biology, University of Belgrade – Faculty of Biology, Belgrade, Serbia; VIBHA BHARDWAJ, Ras Al Khaimah Municipality Department, Director Environment Laboratories, Dubai, United Arab Emirate; VALERIA CARPENTIERI PIPOLO, CNPT; NEMANJA KUZMANOVIĆ, Federal Research Centre for Cultivated Plants (JKI), Institute for Plant Protection in Horticulture and Forests, Julius Ku¨ hn-Institut, Braunschweig, Germany; GIULIANO DEGRASSI, Industrial Biotechnology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Buenos Aires, Argentina. |
|
Título: |
The chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
Plos One, v. 16, n.11, e0259465 Jan. 2021. |
|
DOI: |
https://doi.org/10.1371/journal. pone.0259465 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Curtobacterium sp. GD1 was isolated from leaves of conventionally grown soybean in Brazil. It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp. GD1 showed a strong chitinase activity. The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium. The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg. Furthermore, Curtobacterium sp. GD1 genome was sequenced and analyzed. Our isolate formed a phylogenetic cluster with four other Curtobacterium spp. strains, with ANIb/ANIm � 98%, representing a new, still non described Curtobacterium species. The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved. The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed. Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified. The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp. GD1 suggests that it has the tools for the hydrolysis of different polysaccharides. Therefore, Curtobacterium sp. GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader. MenosCurtobacterium sp. GD1 was isolated from leaves of conventionally grown soybean in Brazil. It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp. GD1 showed a strong chitinase activity. The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium. The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg. Furthermore, Curtobacterium sp. GD1 genome was sequenced and analyzed. Our isolate formed a phylogenetic cluster with four other Curtobacterium spp. strains, with ANIb/ANIm � 98%, representing a new, still non described Curtobacterium species. The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved. The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed. Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified. The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp. GD1 suggests that it has the tools for the hydrolysis of different polysaccharides.... Mostrar Tudo |
|
Palavras-Chave: |
Curtobacterium sp; Enzyme; GD1. |
|
Thesaurus Nal: |
Soybeans. |
|
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/228529/1/journal.pone.0259465-PLOSONE-2021.pdf
|
|
Marc: |
LEADER 02369naa a2200229 a 4500
001 2137133
005 2021-12-06
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal. pone.0259465$2DOI
100 1 $aDIMKIĆ, I.
245 $aThe chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence.$h[electronic resource]
260 $c2021
520 $aCurtobacterium sp. GD1 was isolated from leaves of conventionally grown soybean in Brazil. It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp. GD1 showed a strong chitinase activity. The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium. The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg. Furthermore, Curtobacterium sp. GD1 genome was sequenced and analyzed. Our isolate formed a phylogenetic cluster with four other Curtobacterium spp. strains, with ANIb/ANIm � 98%, representing a new, still non described Curtobacterium species. The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved. The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed. Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified. The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp. GD1 suggests that it has the tools for the hydrolysis of different polysaccharides. Therefore, Curtobacterium sp. GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.
650 $aSoybeans
653 $aCurtobacterium sp
653 $aEnzyme
653 $aGD1
700 1 $aBHARDWAJ, V.
700 1 $aCARPENTIERI-PIPOLO, V.
700 1 $aKUZMANOVIĆ, N.
700 1 $aDEGRASSI, G.
773 $tPlos One$gv. 16, n.11, e0259465 Jan. 2021.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Trigo (CNPT) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 65 | |
| 22. |  | SEIFERT, A. L.; CARPENTIERI-PÍPOLO, V.; FERREIRA, J. M.; GERAGE, A. C. Análise combinatória de populações de milho pipoca em topcrosses. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 41, n. 5, p. 771-778, maio 2006 Título em inglês: Combining ability analysis of popcorn populations in topcrosses.| Biblioteca(s): Embrapa Unidades Centrais. |
|    |
| 31. | | SILVA, G. da; CARPENTIERI-PIPOLO, V.; RIEDER, R.; PATRICIO, D. I.; VILARINHO, A. A. Uso de ferramenta de bioinformática para visualização de dados de pedigree na pesquisa e melhoramento de cevada. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 16.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 13., 2021, Passo Fundo. Anais... Passo Fundo: Embrapa Trigo, 2021.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
| |
| 32. | | CARPENTIERI-PIPOLO, V.; KIIHL, T. A. M.; PATRICIO, D. I.; SCHEEREN, P. L.; BONOW, S. Estimativa da variabilidade genética, baseada no coeficiente de parentesco e na divergência genética de caracteres, para compor mini-coleção nuclear de trigo. In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 13., 2019, Passo Fundo. Ata e Resumos... Passo Fundo: Ed. do Autor, 2019. p. 318-323.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
| |
| 33. | | CARPENTIERI-PIPOLO, V.; ALMEIDA, L. A. de; KIIHL, R. A. de S.; PAGLIOSA, E. S. Inheritance of late flowering in natural variants of soybean cultivars under short-day confitions. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 49, n. 10, p. 796-803, out. 2014. Título em português: Herança do florescimento tardio em variantes naturais de cultivares de soja em condições de dias curtos.| Biblioteca(s): Embrapa Unidades Centrais. |
| |
| 37. | | BONFANTE, N.; KIIHL, T. A. M.; CARPENTIERI-PIPOLO, V.; BRAMMER, S. P. Intercâmbio e conservação de germoplasma na Embrapa Trigo. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 15.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 12., 2020, Passo Fundo. Resumos... Brasília, DF: Embrapa, p. 23, 2021.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
| |
| Registros recuperados : 65 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
