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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
14/10/2009 |
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Data da última atualização: |
13/07/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARTINS, F. A.; CARNEIRO, P. C. S.; GUIMARAES, C. T.; MAGALHAES, J. V. D.; CARNEIRO, J. E. S.; CRUZ, C. D. |
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Afiliação: |
Francielle Alline Martins, Universidade Federal de Viçosa; Pedro Crescêncio Souza Carneiro, Universidade Federal de Viçosa; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; JURANDIR VIEIRA DE MAGALHAES, CNPMS; José Eustáquio de Souza Caneiro, Universidade Federal de Viçosa; Cosme Damião Cruz, Universidade Federal de Viçosa. |
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Título: |
Distinction between plant samples according to allele dosage by semiquantitative polymerase chain reaction. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 8, n. 1, p. 319-327, 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
The lack of informativity of samples from heterozygotic individuals is one of the hindrances in the mapping of quantitative trait loci of outbred populations, since it is not normally possible to identify the origin of each allele. One way to include these individuals in analyses would be to genotype their endosperm, considering that heterozygote (Aa) has AAa endosperm, when the female genitor donates the A or a allele, respectivelly. We used semiquantitative polymerase chain reaction to determine allele dosages in DNA mixtures, by simulating the observed conditions for endospermic tissue. Semiquantitative polymerase chain reaction on agarose gels, along with regression analysis, allowed differentiation of the samples according to the amount of DNA. This type of information will help decrease the number of non-informative individuals in quantitative trait locus mapping of outbred populations, thereby increasing mapping accuracy. |
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Thesagro: |
Genética vegetal; Milho; Zea mays. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/59883/1/Distinction-between.pdf
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Marc: |
LEADER 01624naa a2200217 a 4500
001 1513749
005 2018-07-13
008 2009 bl uuuu u00u1 u #d
100 1 $aMARTINS, F. A.
245 $aDistinction between plant samples according to allele dosage by semiquantitative polymerase chain reaction.$h[electronic resource]
260 $c2009
520 $aThe lack of informativity of samples from heterozygotic individuals is one of the hindrances in the mapping of quantitative trait loci of outbred populations, since it is not normally possible to identify the origin of each allele. One way to include these individuals in analyses would be to genotype their endosperm, considering that heterozygote (Aa) has AAa endosperm, when the female genitor donates the A or a allele, respectivelly. We used semiquantitative polymerase chain reaction to determine allele dosages in DNA mixtures, by simulating the observed conditions for endospermic tissue. Semiquantitative polymerase chain reaction on agarose gels, along with regression analysis, allowed differentiation of the samples according to the amount of DNA. This type of information will help decrease the number of non-informative individuals in quantitative trait locus mapping of outbred populations, thereby increasing mapping accuracy.
650 $aGenética vegetal
650 $aMilho
650 $aZea mays
700 1 $aCARNEIRO, P. C. S.
700 1 $aGUIMARAES, C. T.
700 1 $aMAGALHAES, J. V. D.
700 1 $aCARNEIRO, J. E. S.
700 1 $aCRUZ, C. D.
773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 8, n. 1, p. 319-327, 2009.
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Embrapa Milho e Sorgo (CNPMS) |
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