|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
07/08/2020 |
Data da última atualização: |
27/10/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PINHEIRO, D. H.; MOREIRA, R. O.; LEITE, N. A.; REDOAN, A. C.; XAVIER, A. da S.; BARROS, B. de A.; CARNEIRO, N. P. |
Afiliação: |
Daniele Heloisa Pinheiro; Raquel Oliveira Moreira, UNESP; Natalia Alves Leite, Universidade Federal do Rio Grande do Sul; Ana Carolina Redoan; Andre da Silva Xavier, Universidade Federal do Espírito Santo; BEATRIZ DE ALMEIDA BARROS, CNPMS; NEWTON PORTILHO CARNEIRO, CNPMS. |
Título: |
Suitable reference genes for RT-qPCR analysis in Dichelops melacanthus (Hemiptera: Pentatomidae). |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Molecular Biology Reports, v. 47, p. 4989-5000, 2020. |
DOI: |
10.1007/s11033-020-05550-z |
Idioma: |
Inglês |
Conteúdo: |
The relative quantification of gene expression is mainly realized through reverse transcription-quantitative PCR (RT-qPCR). However, the accuracy of this technique is deeply influenced by the expression stability of the reference genes used for data normalization. Therefore, the selection of suitable reference genes for a given experimental condition is a prerequisite in gene expression studies.Dichelops melacanthus(Hemiptera: Pentatomidae) is an important phloem sap-sucking insect pest of soybean, wheat, and maize in Brazil. Most of the genetic and molecular biology studies require gene expression analysis. Nevertheless, there are no reports about reference genes for RT-qPCR data normalization inD. melacanthus. In this study, we evaluated the expression stability of nine candidate reference genes (nadh,sdhb,gapdh,fau,ef1a,rpl9,ube4a,gusandrps23) in different developmental stages, body parts, sex, starvation-induced stress and dsRNA exposure by RefFinder software that integrates the statistical algorithms geNorm, NormFinder, BestKeeper, and Delta Ct method. Our results showed that ef1a and nadh are the most stable reference genes for developmental stages,fauandrps23for sex,ube4aandrps23for body parts,rpl9andfaufor starvation stress, andnadhandsdhbfor dsRNA exposure treatment. The reference genes selected in this work will be useful for further RT-qPCR analyses onD. melacanthus, facilitating future gene expression studies that can provide a better understanding of the developmental, physiological, and molecular processes of this important insect pest. Moreover, the knowledge gained from these studies can be helpful to design effective and sustainable pest management strategies. MenosThe relative quantification of gene expression is mainly realized through reverse transcription-quantitative PCR (RT-qPCR). However, the accuracy of this technique is deeply influenced by the expression stability of the reference genes used for data normalization. Therefore, the selection of suitable reference genes for a given experimental condition is a prerequisite in gene expression studies.Dichelops melacanthus(Hemiptera: Pentatomidae) is an important phloem sap-sucking insect pest of soybean, wheat, and maize in Brazil. Most of the genetic and molecular biology studies require gene expression analysis. Nevertheless, there are no reports about reference genes for RT-qPCR data normalization inD. melacanthus. In this study, we evaluated the expression stability of nine candidate reference genes (nadh,sdhb,gapdh,fau,ef1a,rpl9,ube4a,gusandrps23) in different developmental stages, body parts, sex, starvation-induced stress and dsRNA exposure by RefFinder software that integrates the statistical algorithms geNorm, NormFinder, BestKeeper, and Delta Ct method. Our results showed that ef1a and nadh are the most stable reference genes for developmental stages,fauandrps23for sex,ube4aandrps23for body parts,rpl9andfaufor starvation stress, andnadhandsdhbfor dsRNA exposure treatment. The reference genes selected in this work will be useful for further RT-qPCR analyses onD. melacanthus, facilitating future gene expression studies that can provide a better understanding of the develop... Mostrar Tudo |
Thesagro: |
Gene; Inseto; Percevejo; Seleção Genética. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02446naa a2200253 a 4500 001 2124236 005 2020-10-27 008 2020 bl uuuu u00u1 u #d 024 7 $a10.1007/s11033-020-05550-z$2DOI 100 1 $aPINHEIRO, D. H. 245 $aSuitable reference genes for RT-qPCR analysis in Dichelops melacanthus (Hemiptera$bPentatomidae).$h[electronic resource] 260 $c2020 520 $aThe relative quantification of gene expression is mainly realized through reverse transcription-quantitative PCR (RT-qPCR). However, the accuracy of this technique is deeply influenced by the expression stability of the reference genes used for data normalization. Therefore, the selection of suitable reference genes for a given experimental condition is a prerequisite in gene expression studies.Dichelops melacanthus(Hemiptera: Pentatomidae) is an important phloem sap-sucking insect pest of soybean, wheat, and maize in Brazil. Most of the genetic and molecular biology studies require gene expression analysis. Nevertheless, there are no reports about reference genes for RT-qPCR data normalization inD. melacanthus. In this study, we evaluated the expression stability of nine candidate reference genes (nadh,sdhb,gapdh,fau,ef1a,rpl9,ube4a,gusandrps23) in different developmental stages, body parts, sex, starvation-induced stress and dsRNA exposure by RefFinder software that integrates the statistical algorithms geNorm, NormFinder, BestKeeper, and Delta Ct method. Our results showed that ef1a and nadh are the most stable reference genes for developmental stages,fauandrps23for sex,ube4aandrps23for body parts,rpl9andfaufor starvation stress, andnadhandsdhbfor dsRNA exposure treatment. The reference genes selected in this work will be useful for further RT-qPCR analyses onD. melacanthus, facilitating future gene expression studies that can provide a better understanding of the developmental, physiological, and molecular processes of this important insect pest. Moreover, the knowledge gained from these studies can be helpful to design effective and sustainable pest management strategies. 650 $aGene 650 $aInseto 650 $aPercevejo 650 $aSeleção Genética 700 1 $aMOREIRA, R. O. 700 1 $aLEITE, N. A. 700 1 $aREDOAN, A. C. 700 1 $aXAVIER, A. da S. 700 1 $aBARROS, B. de A. 700 1 $aCARNEIRO, N. P. 773 $tMolecular Biology Reports$gv. 47, p. 4989-5000, 2020.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
03/09/2015 |
Data da última atualização: |
03/07/2019 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
CASULA. D. K.; KRZYZANOWSKI, F. C. |
Afiliação: |
FRANCISCO CARLOS KRZYZANOWSKI, CNPSO. |
Título: |
Análise de lignina em diferentes partes da soja utilizando o método do brometo de acetila. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 10., 2015, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2015. |
Páginas: |
p. 94-100. |
Idioma: |
Português |
Palavras-Chave: |
Método do brometo de acetila. |
Thesagro: |
Soja. |
Thesaurus NAL: |
Soybeans. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/129012/1/JA.p.94-100.pdf
|
Marc: |
LEADER 00567nam a2200157 a 4500 001 2023228 005 2019-07-03 008 2015 bl uuuu u00u1 u #d 100 1 $aCASULA. D. K. 245 $aAnálise de lignina em diferentes partes da soja utilizando o método do brometo de acetila.$h[electronic resource] 260 $aIn: JORNADA ACADÊMICA DA EMBRAPA SOJA, 10., 2015, Londrina. Resumos expandidos... Londrina: Embrapa Soja$c2015 300 $ap. 94-100. 650 $aSoybeans 650 $aSoja 653 $aMétodo do brometo de acetila 700 1 $aKRZYZANOWSKI, F. C.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Soja (CNPSO) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|