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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
20/10/2016 |
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Data da última atualização: |
20/10/2016 |
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Autoria: |
SAHLU, T.; JACQUEMET, N.; CARNEIRO, H.; HART, S. P.; KLEIN, P. D. |
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Título: |
Application of 13CO2 measurements to the estimation of energy expenditure in goats. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Canadian Journal of Animal Science, v. 72, n. 2, p. 245-252, Jun. 1992. |
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DOI: |
10.4141/cjas92-031 |
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Idioma: |
Inglês |
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Conteúdo: |
The use and limitations of 13C stable isotopes as tracers in animal experimentation was investigated using 13C-labeled bicarbonate to estimate CO2 production and energetic requirement of free-ranging goats. Goats were fed wheat hay (Triticum aestivum; C3) or Old World Bluestem hay (Bothriochloa caucasica; C4; OWBS) ad libitum for 21 d. Parotid salivary ducts were cannulated for saliva collection. Isotopic bicarbonate (13C) was infused at a constant rate into the peritoneal cavity. In exp. 1, saliva samples were obtained at successive 2-h periods for 20 h to establish the time to achieve equilibrium. The half-life of bicarbonate was 2.9 h for animals consuming the wheat diet and 4.1 h (P < 0.06) for animals consuming OWBS diet. The time required to reach equilibrium was 12 h for animals on the wheat diet and 16 h for animals on OWBS diet. The error in the technique arising from animals consuming C3 vs. C4 species (which have different endogenous 13C contents) was studied in exp. 2. If species composition (C3 vs. C4) of the diet remains constant within 10%, the error is negligible. In exp. 3, estimation of CO2 production from continuous saliva samples had a lower coefficient of variation (6.3 vs. 10.0%) than measurements based on spot serum samples. Energy expenditure of goats in stalls consuming hay ad libitum was similar to that of ad-libitum-fed sheep at similar body weights. The 13C methodology appears to be a promising method for measuring energy expenditure of grazing animals. MenosThe use and limitations of 13C stable isotopes as tracers in animal experimentation was investigated using 13C-labeled bicarbonate to estimate CO2 production and energetic requirement of free-ranging goats. Goats were fed wheat hay (Triticum aestivum; C3) or Old World Bluestem hay (Bothriochloa caucasica; C4; OWBS) ad libitum for 21 d. Parotid salivary ducts were cannulated for saliva collection. Isotopic bicarbonate (13C) was infused at a constant rate into the peritoneal cavity. In exp. 1, saliva samples were obtained at successive 2-h periods for 20 h to establish the time to achieve equilibrium. The half-life of bicarbonate was 2.9 h for animals consuming the wheat diet and 4.1 h (P < 0.06) for animals consuming OWBS diet. The time required to reach equilibrium was 12 h for animals on the wheat diet and 16 h for animals on OWBS diet. The error in the technique arising from animals consuming C3 vs. C4 species (which have different endogenous 13C contents) was studied in exp. 2. If species composition (C3 vs. C4) of the diet remains constant within 10%, the error is negligible. In exp. 3, estimation of CO2 production from continuous saliva samples had a lower coefficient of variation (6.3 vs. 10.0%) than measurements based on spot serum samples. Energy expenditure of goats in stalls consuming hay ad libitum was similar to that of ad-libitum-fed sheep at similar body weights. The 13C methodology appears to be a promising method for measuring energy expenditure of grazing an... Mostrar Tudo |
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Palavras-Chave: |
Isotopo estável; Nutritional requirements. |
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Thesagro: |
Bicarbonato de sódio; Dióxido de carbono; Exigência nutricional; Nutrição animal; Ovino. |
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Thesaurus Nal: |
Carbon dioxide; Sheep; Sodium bicarbonate; stable isotopes. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02400naa a2200313 a 4500
001 2055081
005 2016-10-20
008 1992 bl uuuu u00u1 u #d
024 7 $a10.4141/cjas92-031$2DOI
100 1 $aSAHLU, T.
245 $aApplication of 13CO2 measurements to the estimation of energy expenditure in goats.
260 $c1992
520 $aThe use and limitations of 13C stable isotopes as tracers in animal experimentation was investigated using 13C-labeled bicarbonate to estimate CO2 production and energetic requirement of free-ranging goats. Goats were fed wheat hay (Triticum aestivum; C3) or Old World Bluestem hay (Bothriochloa caucasica; C4; OWBS) ad libitum for 21 d. Parotid salivary ducts were cannulated for saliva collection. Isotopic bicarbonate (13C) was infused at a constant rate into the peritoneal cavity. In exp. 1, saliva samples were obtained at successive 2-h periods for 20 h to establish the time to achieve equilibrium. The half-life of bicarbonate was 2.9 h for animals consuming the wheat diet and 4.1 h (P < 0.06) for animals consuming OWBS diet. The time required to reach equilibrium was 12 h for animals on the wheat diet and 16 h for animals on OWBS diet. The error in the technique arising from animals consuming C3 vs. C4 species (which have different endogenous 13C contents) was studied in exp. 2. If species composition (C3 vs. C4) of the diet remains constant within 10%, the error is negligible. In exp. 3, estimation of CO2 production from continuous saliva samples had a lower coefficient of variation (6.3 vs. 10.0%) than measurements based on spot serum samples. Energy expenditure of goats in stalls consuming hay ad libitum was similar to that of ad-libitum-fed sheep at similar body weights. The 13C methodology appears to be a promising method for measuring energy expenditure of grazing animals.
650 $aCarbon dioxide
650 $aSheep
650 $aSodium bicarbonate
650 $astable isotopes
650 $aBicarbonato de sódio
650 $aDióxido de carbono
650 $aExigência nutricional
650 $aNutrição animal
650 $aOvino
653 $aIsotopo estável
653 $aNutritional requirements
700 1 $aJACQUEMET, N.
700 1 $aCARNEIRO, H.
700 1 $aHART, S. P.
700 1 $aKLEIN, P. D.
773 $tCanadian Journal of Animal Science$gv. 72, n. 2, p. 245-252, Jun. 1992.
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Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 96 | |
| 16. |  | CARNEIRO, H.; ARAUJO FILHO, J. A.; CRISPIM, S. M. A. Goat diet in a lowered Caatinga. In: INTERNATIONAL CONFERENCE ON GOATS, 4., 1987, Brasília, DF. Proceedings... Brasília, DF: EMBRAPA-DDT, 1987. v. 2, p. 1434-1435. Resumo 188.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 96 | |
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