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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
20/11/2012 |
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Data da última atualização: |
30/01/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, J. R. P.; ANDRADE, E. P.; COSTA, D. da C.; GONZAGA, V.; CARES, J. E. |
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Afiliação: |
JANSEN R.P. SANTOS, UnB; EDNALVA P. ANDRADE, UnB; DILSON DA CUNHA COSTA, CENARGEN; VILMAR GONZAGA, CENARGEN; JUVENIL E. CARES, UnB. |
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Título: |
Comparison of two methods for in vitro multiplication of Radopholus similis and Pratylenchus brachyurus in carrot cylinders. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 37, n. 4, p. 266-270, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The use of in vitro mass multiplication of nematodes under axenic conditions allows intensification of studies on taxonomy, biology, epidemiology and control of these pathogens. In the present study, two methods were compared for in vitro multiplication of two species of plant-parasitic nematodes, Radopholus similis and Pratylenchus brachyurus in 20, 40 and 60 day-periods. Both techniques involved the use of carrot cylinders placed in jars with lids, with water–agar in the bottom of the flasks (WA+) or without (WA-). In WA- the nematodes were treated with a 1% solution of ampicillin whilst for WA+ the axenization was carried out with a solution of mercuric chloride (0.01%), and streptomycin sulfate 0.02%. The WA+ condition was the most favorable for multiplication of both species of nematodes, resulting in a population increase of about 280 times for R. similis and 226 times for P. brachyurus in relation to the founding population of 25 individuals. For WA- a population increase of only five times after 60 days for R. similis and a duplication of the population after 60 days for P. brachyurus was obtained. Overall, the largest amount of nematodes occurred at 60 days after inoculation. |
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Palavras-Chave: |
Burrowing nematode; In vitro multiplication; Multiplicação in vitro. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01848naa a2200205 a 4500
001 1940056
005 2013-01-30
008 2012 bl uuuu u00u1 u #d
100 1 $aSANTOS, J. R. P.
245 $aComparison of two methods for in vitro multiplication of Radopholus similis and Pratylenchus brachyurus in carrot cylinders.
260 $c2012
520 $aThe use of in vitro mass multiplication of nematodes under axenic conditions allows intensification of studies on taxonomy, biology, epidemiology and control of these pathogens. In the present study, two methods were compared for in vitro multiplication of two species of plant-parasitic nematodes, Radopholus similis and Pratylenchus brachyurus in 20, 40 and 60 day-periods. Both techniques involved the use of carrot cylinders placed in jars with lids, with water–agar in the bottom of the flasks (WA+) or without (WA-). In WA- the nematodes were treated with a 1% solution of ampicillin whilst for WA+ the axenization was carried out with a solution of mercuric chloride (0.01%), and streptomycin sulfate 0.02%. The WA+ condition was the most favorable for multiplication of both species of nematodes, resulting in a population increase of about 280 times for R. similis and 226 times for P. brachyurus in relation to the founding population of 25 individuals. For WA- a population increase of only five times after 60 days for R. similis and a duplication of the population after 60 days for P. brachyurus was obtained. Overall, the largest amount of nematodes occurred at 60 days after inoculation.
653 $aBurrowing nematode
653 $aIn vitro multiplication
653 $aMultiplicação in vitro
700 1 $aANDRADE, E. P.
700 1 $aCOSTA, D. da C.
700 1 $aGONZAGA, V.
700 1 $aCARES, J. E.
773 $tTropical Plant Pathology$gv. 37, n. 4, p. 266-270, 2012.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
22/11/2018 |
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Data da última atualização: |
22/11/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
FERNANDES, F. S.; GODOY, W. A. C.; RAMALHO, F. de S.; GARCIA A. G.; SANTOS, B. D. B. dos; MALAQUIAS, J. B. |
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Afiliação: |
FRANCISCO S. FERNANDES, ESALQ; WESLEY A. C. GODOY, ESALQ; FRANCISCO DE SOUSA RAMALHO, CNPA; ADRIANO G. GARCIA, ESALQ; BÁBARA DAVIS BRITO dos SANTOS; JOSE BRUNO MALAQUIAS, CNPA. |
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Título: |
Population dynamics of Aphis gossypii Glover and Aphis craccivora Koch (Hemiptera: Heteroptera: Aphididae) in sole and intercropping systems of cotton and cowpea. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Anais da Academia Brasileira de Ciências, v. 90, n. 1, p. 311-323, 2018. |
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ISSN: |
0001-3765 |
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Idioma: |
Inglês |
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Conteúdo: |
Population dynamics of aphids have been studied in sole and intercropping systems. These studies have required the use of more precise analytical tools in order to better understand patterns in quantitative data. Mathematical models are among the most important tools to explain the dynamics of insect populations. This study investigated the population dynamics of aphids Aphis gossypii and Aphis craccivora over time, using mathematical models composed of a set of differential equations as a helpful analytical tool to understand the population dynamics of aphids in arrangements of cotton and cowpea. The treatments were sole cotton, sole cowpea, and three arrangements of cotton intercropped with cowpea (t1, t2 and t3). The plants were infested with two aphid species and were evaluated at 7, 14, 28, 35, 42, and 49 days after the infestations. Mathematical models were used to fit the population dynamics of two aphid species. There were good fits for aphid dynamics by mathematical model over time. The highest population peak of both species A. gossypii and A. craccivora was found in the sole crops, and the lowest population peak was found in crop system t2. These results are important for integrated management programs of aphids in cotton and cowpea. |
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Thesagro: |
Aphis Craccivora; Aphis Gossypii; Hemíptera. |
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Thesaurus NAL: |
Cotton. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/186791/1/Population-dynamics-of.pdf
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Marc: |
LEADER 02054naa a2200241 a 4500
001 2099877
005 2018-11-22
008 2018 bl uuuu u00u1 u #d
022 $a0001-3765
100 1 $aFERNANDES, F. S.
245 $aPopulation dynamics of Aphis gossypii Glover and Aphis craccivora Koch (Hemiptera$bHeteroptera: Aphididae) in sole and intercropping systems of cotton and cowpea.$h[electronic resource]
260 $c2018
520 $aPopulation dynamics of aphids have been studied in sole and intercropping systems. These studies have required the use of more precise analytical tools in order to better understand patterns in quantitative data. Mathematical models are among the most important tools to explain the dynamics of insect populations. This study investigated the population dynamics of aphids Aphis gossypii and Aphis craccivora over time, using mathematical models composed of a set of differential equations as a helpful analytical tool to understand the population dynamics of aphids in arrangements of cotton and cowpea. The treatments were sole cotton, sole cowpea, and three arrangements of cotton intercropped with cowpea (t1, t2 and t3). The plants were infested with two aphid species and were evaluated at 7, 14, 28, 35, 42, and 49 days after the infestations. Mathematical models were used to fit the population dynamics of two aphid species. There were good fits for aphid dynamics by mathematical model over time. The highest population peak of both species A. gossypii and A. craccivora was found in the sole crops, and the lowest population peak was found in crop system t2. These results are important for integrated management programs of aphids in cotton and cowpea.
650 $aCotton
650 $aAphis Craccivora
650 $aAphis Gossypii
650 $aHemíptera
700 1 $aGODOY, W. A. C.
700 1 $aRAMALHO, F. de S.
700 1 $aGARCIA A. G.
700 1 $aSANTOS, B. D. B. dos
700 1 $aMALAQUIAS, J. B.
773 $tAnais da Academia Brasileira de Ciências$gv. 90, n. 1, p. 311-323, 2018.
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Embrapa Algodão (CNPA) |
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