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Registro Completo |
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Café. |
Data corrente: |
05/02/2015 |
Data da última atualização: |
06/02/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CARDOSO, D. C.; MARTINATI, J. C.; GIACHETTO, P. F.; VIDAL, R. O.; CARAZZOLLE, M. F.; PADILHA, L.; GUERREIRO-FILHO, O.; MALUF, M. P. |
Afiliação: |
DANIELLE C. CARDOSO, IAC; JULIANA C MARTINATI, IAC; POLIANA FERNANDA GIACHETTO, CNPTIA; RAMON O. VIDAL; MARCELO F. CARAZZOLLE; LILIAN PADILHA, SAPC; OLIVEIRO GUERREIRO-FILHO, IAC; MIRIAN PEREZ MALUF, SAPC. |
Título: |
Large-scale analysis of differential gene expression in coffee genotypes resistant and susceptible to leaf miner-toward the identification of candidate genes for marker assisted-selection. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
BMC Genomics, London, v. 15, p. 1-20, 2014. |
DOI: |
10.1186/1471-2164-15-66 |
Idioma: |
Inglês |
Conteúdo: |
A successful development of herbivorous insects into plant tissues depends on coordination of metabolic processes. Plants have evolved complex mechanisms to recognize such attacks, and to trigger a defense response. To understand the transcriptional basis of this response, we compare gene expression profiles of two coffee genotypes, susceptible and resistant to leaf miner (Leucoptera coffella). A total of 22000 EST sequences from the Coffee Genome Database were selected for a microarray analysis. Fluorescence probes were synthesized using mRNA from the infested and non-infested coffee plants. Array hybridization, scanning and data normalization were performed using Nimble Scan® e ArrayStar® platforms. Genes with foldchange values +/-2 were considered differentially expressed. A validation of 18 differentially expressed genes was performed in infected plants using qRT-PCR approach. |
Palavras-Chave: |
Microarranjo; Plant defense. |
Thesagro: |
Café; Coffea Arábica. |
Thesaurus Nal: |
Genes; Leafminers; Microarray technology. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/117310/1/Large-scale-analysis.pdf
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Marc: |
LEADER 01841naa a2200301 a 4500 001 2007801 005 2015-02-06 008 2014 bl uuuu u00u1 u #d 024 7 $a10.1186/1471-2164-15-66$2DOI 100 1 $aCARDOSO, D. C. 245 $aLarge-scale analysis of differential gene expression in coffee genotypes resistant and susceptible to leaf miner-toward the identification of candidate genes for marker assisted-selection.$h[electronic resource] 260 $c2014 520 $aA successful development of herbivorous insects into plant tissues depends on coordination of metabolic processes. Plants have evolved complex mechanisms to recognize such attacks, and to trigger a defense response. To understand the transcriptional basis of this response, we compare gene expression profiles of two coffee genotypes, susceptible and resistant to leaf miner (Leucoptera coffella). A total of 22000 EST sequences from the Coffee Genome Database were selected for a microarray analysis. Fluorescence probes were synthesized using mRNA from the infested and non-infested coffee plants. Array hybridization, scanning and data normalization were performed using Nimble Scan® e ArrayStar® platforms. Genes with foldchange values +/-2 were considered differentially expressed. A validation of 18 differentially expressed genes was performed in infected plants using qRT-PCR approach. 650 $aGenes 650 $aLeafminers 650 $aMicroarray technology 650 $aCafé 650 $aCoffea Arábica 653 $aMicroarranjo 653 $aPlant defense 700 1 $aMARTINATI, J. C. 700 1 $aGIACHETTO, P. F. 700 1 $aVIDAL, R. O. 700 1 $aCARAZZOLLE, M. F. 700 1 $aPADILHA, L. 700 1 $aGUERREIRO-FILHO, O. 700 1 $aMALUF, M. P. 773 $tBMC Genomics, London$gv. 15, p. 1-20, 2014.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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Registros recuperados : 10 | |
3. | | MARTINATI, J. C.; CARDOSO, D. C.; GUERREIRO-FILHO, O.; VIDAL, R. O.; CARAZZOLLE, M. F.; MALUF, M. P. Caracterização molecular da interação cafeeiro/bicho-mineiro. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 7., 2011, Araxá. Anais... Brasília, DF: Embrapa Café, 2011.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Café. |
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4. | | RODRIGUES, T. B.; KHAJURIA, C.; WANG, H.; MATZ, N.; CARDOSO, D. C.; VALICENTE, F. H.; ZHOU, X.; SIEGFRIED, B. Validation of reference housekeeping genes for gene expression studies in western corn rootworm (Diabrotica virgifera virgifera). Plos One, San Francisco,v. 9, n. 10, p. 1-8, Oct. 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Milho e Sorgo. |
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5. | | PADILHA, L.; SCHENK, J. C. M.; ORTEGA, H. M.; CARDOSO, D. C.; KAMIMURA, E. H.; ROSA, S. D. V. F.; MALUF, M. P. Expressão de genes relacionados ao estresse oxidativo em sementes de café armazenadas. In: CONGRESSO BRASILEIRO DE SEMENTES, 18., 2013, Florianópolis, SC. A semente na produtividade agrícola e na conservação de recursos genéticos. Londrina, PR: Associação Brasileira de Tecnologia de Sementes, 2013.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Café. |
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6. | | MARTINATI, J. C.; CARDOSO, D. C.; VIDAL, R. O.; CARAZZOLLE, M. F.; GIACHETTO, P. F.; PADILHA, L.; GUERREIRO-FILHO, O.; MALUF, M. P. Large-escale expression of genes related to phytoalexins, phenols, flavonoids and lignin biosynthesis in coffee plants infested with leaf-miner. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 24., 2012, Costa Rica. Programme & abstracts. [S.l.]: Association for Science and Information on Coffee, 2012. p. 83. B11. ASIC 2012.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agricultura Digital. |
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7. | | MARTINATI, J. C.; CARDOSO, D. C.; VIDAL, R. O.; CARAZZOLLE, M. F.; GIACHETTO, P. F.; PADILHA, L.; OLIVEIRA FILHO, G.; MALUF, M. P. Large-escale expression of genes related to phytoalexins, phenols, flavonoids and lignin biosynthesis in coffee plants infested with leaf-miner. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 24., 2012, Costa Rica. Programme & abstracts. [S.l.]: Association for Science and Information on Coffee, 2012. p. 83 B11. ASIC 2012.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Café. |
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8. | | CARDOSO, D. C.; MARTINATI, J. C.; GIACHETTO, P. F.; VIDAL, R. O.; CARAZZOLLE, M. F.; PADILHA, L.; GUERREIRO-FILHO, O.; MALUF, M. P. Large-scale analysis of differential gene expression in coffee genotypes resistant and susceptible to leaf miner-toward the identification of candidate genes for marker assisted-selection. BMC Genomics, London, v. 15, p. 1-20, 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Agricultura Digital; Embrapa Café. |
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9. | | GHINI, R.; MAC LEOD, R. E. de O.; TORRE NETO, A.; CARDOSO, D. C.; BETTIOL, W.; MORAIS, L. A. S. de; VIQUE, B. Increased atmospheric carbon dioxide concentration: effects on eucalypt rust (Puccinia psidii), C:N ratio and essential oils in eucalypt clonal plantlets. Forest Pathology, Hamburg, v. 44, n. 5, p. 409-416, 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Instrumentação; Embrapa Meio Ambiente. |
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10. | | SANTOS, L. L. dos; PROSDOCIMI, F.; LIMA, N. C. B.; COSTA, I. R. da; CARDOSO, D. C.; DRUMMOND, M. G.; BRASIL, B. dos S. A. F.; BASTIANETTO, E.; OLIVEIRA, D. A. A. de. Comparative genomics and phylogenomics of Trichostrongyloidea mitochondria reveal insights for molecular diagnosis and evolutionary biology of nematode worms. Gene Reports, v. 9, p. 65-73, 2017.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
Biblioteca(s): Embrapa Agroenergia. |
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Registros recuperados : 10 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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