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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
14/12/2016 |
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Data da última atualização: |
03/10/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARDOSO, A. A.; ANDRAUS, M. de P.; BORBA, T. C. de O.; MARTIN-DIDONET, C. C. G.; FERREIRA, E. P. de B. |
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Afiliação: |
ALINE ASSIS CARDOSO, UFG; MICHEL DE PAULA ANDRAUS, UFG; TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; CLAUDIA CRISTINA GARCIA MARTIN-DIDONET, UNIVERSIDADE ESTADUAL DE GOIÁS, Anápolis-GO; ENDERSON PETRONIO DE BRITO FERREIRA, CNPAF. |
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Título: |
Characterization of rhizobia isolates obtained from nodules of wild genotypes of common bean. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 48, n. 1, p. 43-50, jan./mar. 2017. |
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ISSN: |
1678-4405 |
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DOI: |
10.1016/j.bjm.2016.09.002 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to evaluate the tolerance to salinity and temperature, the genetic diversityand the symbiotic efficiency of rhizobia isolates obtained from wild genotypes of commonbean cultivated in soil samples from the States of Goiás, Minas Gerais and Paraná. The iso-lates were subjected to different NaCl concentrations (0%, 1%, 2%, 4% and 6%) at differenttemperatures (28◦C, 33◦C, 38◦C, 43◦C and 48◦C). Genotypic characterization was performedbased on BOX-PCR, REP-PCR markers and 16S rRNA sequencing. An evaluation of symbioticefficiency was carried out under greenhouse conditions in autoclaved Leonard jars. Among98 isolates about 45% of them and Rhizobium freirei PRF81 showed a high tolerance to tem-perature, while 24 isolates and Rhizobium tropici CIAT899 were able to use all of the carbonsources studied. Clustering analysis based on the ability to use carbon sources and on thetolerance to salinity and temperature grouped 49 isolates, R. tropici CIAT899 and R. tropiciH12 with a similarity level of 76%. Based on genotypic characterization, 65% of the isolatesshowed an approximately 66% similarity with R. tropici CIAT899 and R. tropici H12. About20% of the isolates showed symbiotic efficiency similar to or better than the best Rhizobiumreference strain (R. tropici CIAT899). Phylogenetic analysis of the 16S rRNA revealed that twoefficient isolates (ALSG5A1 and JPrG6A8) belong to the group of strains used as commercialinoculant for common bean in Brazil and must be assayed in field experiments. MenosThis study aimed to evaluate the tolerance to salinity and temperature, the genetic diversityand the symbiotic efficiency of rhizobia isolates obtained from wild genotypes of commonbean cultivated in soil samples from the States of Goiás, Minas Gerais and Paraná. The iso-lates were subjected to different NaCl concentrations (0%, 1%, 2%, 4% and 6%) at differenttemperatures (28◦C, 33◦C, 38◦C, 43◦C and 48◦C). Genotypic characterization was performedbased on BOX-PCR, REP-PCR markers and 16S rRNA sequencing. An evaluation of symbioticefficiency was carried out under greenhouse conditions in autoclaved Leonard jars. Among98 isolates about 45% of them and Rhizobium freirei PRF81 showed a high tolerance to tem-perature, while 24 isolates and Rhizobium tropici CIAT899 were able to use all of the carbonsources studied. Clustering analysis based on the ability to use carbon sources and on thetolerance to salinity and temperature grouped 49 isolates, R. tropici CIAT899 and R. tropiciH12 with a similarity level of 76%. Based on genotypic characterization, 65% of the isolatesshowed an approximately 66% similarity with R. tropici CIAT899 and R. tropici H12. About20% of the isolates showed symbiotic efficiency similar to or better than the best Rhizobiumreference strain (R. tropici CIAT899). Phylogenetic analysis of the 16S rRNA revealed that twoefficient isolates (ALSG5A1 and JPrG6A8) belong to the group of strains used as commercialinoculant for common bean i... Mostrar Tudo |
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Palavras-Chave: |
Carbon source use; Symbiotic efficiency; Toleance to salinity and temperature. |
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Thesagro: |
Feijão; Phaseolus vulgaris; Rhizobium; Simbiose. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/156257/1/CNPAF-2017-bjm.pdf
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Marc: |
LEADER 02432naa a2200277 a 4500
001 2058672
005 2017-10-03
008 2017 bl uuuu u00u1 u #d
022 $a1678-4405
024 7 $a10.1016/j.bjm.2016.09.002$2DOI
100 1 $aCARDOSO, A. A.
245 $aCharacterization of rhizobia isolates obtained from nodules of wild genotypes of common bean.$h[electronic resource]
260 $c2017
520 $aThis study aimed to evaluate the tolerance to salinity and temperature, the genetic diversityand the symbiotic efficiency of rhizobia isolates obtained from wild genotypes of commonbean cultivated in soil samples from the States of Goiás, Minas Gerais and Paraná. The iso-lates were subjected to different NaCl concentrations (0%, 1%, 2%, 4% and 6%) at differenttemperatures (28◦C, 33◦C, 38◦C, 43◦C and 48◦C). Genotypic characterization was performedbased on BOX-PCR, REP-PCR markers and 16S rRNA sequencing. An evaluation of symbioticefficiency was carried out under greenhouse conditions in autoclaved Leonard jars. Among98 isolates about 45% of them and Rhizobium freirei PRF81 showed a high tolerance to tem-perature, while 24 isolates and Rhizobium tropici CIAT899 were able to use all of the carbonsources studied. Clustering analysis based on the ability to use carbon sources and on thetolerance to salinity and temperature grouped 49 isolates, R. tropici CIAT899 and R. tropiciH12 with a similarity level of 76%. Based on genotypic characterization, 65% of the isolatesshowed an approximately 66% similarity with R. tropici CIAT899 and R. tropici H12. About20% of the isolates showed symbiotic efficiency similar to or better than the best Rhizobiumreference strain (R. tropici CIAT899). Phylogenetic analysis of the 16S rRNA revealed that twoefficient isolates (ALSG5A1 and JPrG6A8) belong to the group of strains used as commercialinoculant for common bean in Brazil and must be assayed in field experiments.
650 $aFeijão
650 $aPhaseolus vulgaris
650 $aRhizobium
650 $aSimbiose
653 $aCarbon source use
653 $aSymbiotic efficiency
653 $aToleance to salinity and temperature
700 1 $aANDRAUS, M. de P.
700 1 $aBORBA, T. C. de O.
700 1 $aMARTIN-DIDONET, C. C. G.
700 1 $aFERREIRA, E. P. de B.
773 $tBrazilian Journal of Microbiology, São Paulo$gv. 48, n. 1, p. 43-50, jan./mar. 2017.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 54 | |
| 4. |  | BARRUETO CID, L. P.; OLIVA, M. A.; CARDOSO, A. A. Efeito do potencial hídrico sobre a embebição, a respiração e a germinação da leguminosa Cratylia floribunda. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 16, n. 6, p. 883-890, nov./dez. 1981 Título em inglês: Effect of water potential on imbibition, respiration and germination of leguminous Cratylia floribunda.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 5. | | CID, L. P. B.; OLIVA, M. A.; CARDOSO, A. A. Efeito do potencial hídrico sobre a embebição, a respiração e a germinação da leguminosa Cratylia floribunda. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 16, n. 6, p. 883-890, nov./dez. 1981. Título em inglês: Effect of water potential on imbibition, respiration and germination of leguminous Cratylia floribunda.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 9. | | MOREIRA, L. P.; SAMPAIO, F. B.; CARDOSO, A. A.; FERREIRA, E. P. de B. Caracterização e avaliação agronômica de novos isolados de rizóbio obtidos de nódulos de genótipos silvestes de feijoeiro-comum. In: REUNIÃO DA REDE DE LABORATÓRIOS PARA A RECOMENDAÇÃO, PADRONIZAÇÃO E DIFUSÃO DE TECNOLOGIAS DE INOCULANTES MICROBIANOS DE INTERESSE AGRÍCOLA, 17., 2014, Londrina. Anais... Londrina: Embrapa Soja, 2016. p. 47-48. (Embrapa Soja. Documentos, 369).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 12. | | ARCHANGELO, E. R.; SILVA, A. A. da; SILVA, J. B. da; KARAM, D.; CARDOSO, A. A. Seletividade e eficácia de herbicidas aplicados em pós-emergência na cultura do sorgo forrageiro. Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 1, n. 3, p. 107-115, set./dez. 2002.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 13. | | CARNEIRO, J. E. de S.; BARBOSA, H. M.; CARDOSO, A. A.; VIEIRA, C. Sensibilidade a radiação gama de sementes de Phaseolus vulgaris L., cv. Milionário 1732. Revista Ceres, Vicosa, v. 34, n. 193, p. 306-312, maio/jun. 1987.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 14. | | VASCONCELOS, L. F. L.; CARDOSO, A. A.; COUTO, F. A. de A. Evaluation of guava cultivars as rootstocks, during nursery stage. Acta Horticulturea, n,452, p.63-69, 1997. International Sympisium on Myrtaceae, 1996, Curitiba. Proceedings. Editado por . L.C.Donaldio.| Biblioteca(s): Embrapa Meio-Norte. |
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| Registros recuperados : 54 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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