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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
21/12/2017 |
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Data da última atualização: |
26/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
IVAMOTO, S. T.; REIS JÚNIOR, O.; DOMINGUES, D. S.; SANTOS, T. B. dos; OLIVEIRA, F. F. de; POT, D.; LEROY, T.; VIEIRA, L. G. E.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; PEREIRA, L. F. P. |
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Afiliação: |
Suzana Tiemi Ivamoto, Programa de Pós-Graduação em Genética e Biologia Molecular/Centro de Ciências Bioloógicas/Universidade Estadual de Londrina - UEL; Osvaldo Reis Júnior, Laboratório de Genômica e Expressão/Departamento de Genética/Evolução e Bioagentes/Instituto de Biologia/Universidade Estadual de Campinas - UNICAMP; Douglas Silva Domingues, Departamento de Botânica/Instituto de Biociências de Rio Claro, Universidade Estadual Paulista - UNESP; Tiago Benedito dos Santos, Laboratório de Biotecnologia Vegetal/Instituto Agronômico do Paraná -IAPAR; Fernanda Freitas de Oliveira, Laboratório de Biotecnologia Vegetal/Instituto Agronômico do Paraná -IAPAR; David Pot, CIRAD/UMR AGAP; Thierry Leroy, CIRAD/UMR AGAP; Luiz Gonzaga Esteves Vieira, Programa de Pós Graduação em Agronomia/Universidade do Oeste Paulista - UNOESTE; Marcelo Falsarella Carazzolle, Laboratório de Genômica e Expressão/Departamento de Genética/Evolução e Bioagentes/Instituto de Biologia/Universidade Estadual de Campinas - UNICAMP; Gonçalo Amarante Guimarães Pereira, Laboratório de Genômica e Expressão/Departamento de Genética/Evolução e Bioagentes/Instituto de Biologia/Universidade Estadual de Campinas - UNICAMP; LUIZ FILIPE PROTASIO PEREIRA, SAPC. |
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Título: |
Transcriptome Analysis of Leaves, Flowers and Fruits Perisperm of Coffea arabica L. Reveals the Differential Expression of Genes Involved in Raffinose Biosynthesis. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
PLOS ONE, January 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Coffea arabica L. is an important crop in several developing countries. Despite its economic importance, minimal transcriptome data are available for fruit tissues, especially during fruit development where several compounds related to coffee quality are produced. To understand the molecular aspects related to coffee fruit and grain development, we report a largescale transcriptome analysis of leaf, flower and perisperm fruit tissue development. Illumina sequencing yielded 41,881,572 high-quality filtered reads. De novo assembly generated 65,364 unigenes with an average length of 1,264 bp. A total of 24,548 unigenes were annotated as protein coding genes, including 12,560 full-length sequences. In the annotation process, we identified nine candidate genes related to the biosynthesis of raffinose family oligossacarides (RFOs). These sugars confer osmoprotection and are accumulated during initial fruit development. Four genes from this pathway had their transcriptional pattern validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Furthermore, we identified ~24,000 putative target sites for microRNAs (miRNAs) and 134 putative transcriptionally active transposable elements (TE) sequences in our dataset. This C. arabica transcriptomic atlas provides an important step for identifying candidate genes related to several coffee metabolic pathways, especially those related to fruit chemical composition and therefore beverage quality. Our results are the starting point for enhancing our knowledge about the coffee genes that are transcribed during the flowering and initial fruit development stages. MenosCoffea arabica L. is an important crop in several developing countries. Despite its economic importance, minimal transcriptome data are available for fruit tissues, especially during fruit development where several compounds related to coffee quality are produced. To understand the molecular aspects related to coffee fruit and grain development, we report a largescale transcriptome analysis of leaf, flower and perisperm fruit tissue development. Illumina sequencing yielded 41,881,572 high-quality filtered reads. De novo assembly generated 65,364 unigenes with an average length of 1,264 bp. A total of 24,548 unigenes were annotated as protein coding genes, including 12,560 full-length sequences. In the annotation process, we identified nine candidate genes related to the biosynthesis of raffinose family oligossacarides (RFOs). These sugars confer osmoprotection and are accumulated during initial fruit development. Four genes from this pathway had their transcriptional pattern validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Furthermore, we identified ~24,000 putative target sites for microRNAs (miRNAs) and 134 putative transcriptionally active transposable elements (TE) sequences in our dataset. This C. arabica transcriptomic atlas provides an important step for identifying candidate genes related to several coffee metabolic pathways, especially those related to fruit chemical composition and therefore beverage quality. Our results are the ... Mostrar Tudo |
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Thesagro: |
Coffea Arábica. |
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Thesaurus Nal: |
Biosynthesis; Knowledge; Raffinose. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169661/1/Transcriptome-analysis-of-leaves-flowers.pdf
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Marc: |
LEADER 02512naa a2200289 a 4500
001 2083340
005 2017-12-26
008 2017 bl uuuu u00u1 u #d
100 1 $aIVAMOTO, S. T.
245 $aTranscriptome Analysis of Leaves, Flowers and Fruits Perisperm of Coffea arabica L. Reveals the Differential Expression of Genes Involved in Raffinose Biosynthesis.$h[electronic resource]
260 $c2017
520 $aCoffea arabica L. is an important crop in several developing countries. Despite its economic importance, minimal transcriptome data are available for fruit tissues, especially during fruit development where several compounds related to coffee quality are produced. To understand the molecular aspects related to coffee fruit and grain development, we report a largescale transcriptome analysis of leaf, flower and perisperm fruit tissue development. Illumina sequencing yielded 41,881,572 high-quality filtered reads. De novo assembly generated 65,364 unigenes with an average length of 1,264 bp. A total of 24,548 unigenes were annotated as protein coding genes, including 12,560 full-length sequences. In the annotation process, we identified nine candidate genes related to the biosynthesis of raffinose family oligossacarides (RFOs). These sugars confer osmoprotection and are accumulated during initial fruit development. Four genes from this pathway had their transcriptional pattern validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Furthermore, we identified ~24,000 putative target sites for microRNAs (miRNAs) and 134 putative transcriptionally active transposable elements (TE) sequences in our dataset. This C. arabica transcriptomic atlas provides an important step for identifying candidate genes related to several coffee metabolic pathways, especially those related to fruit chemical composition and therefore beverage quality. Our results are the starting point for enhancing our knowledge about the coffee genes that are transcribed during the flowering and initial fruit development stages.
650 $aBiosynthesis
650 $aKnowledge
650 $aRaffinose
650 $aCoffea Arábica
700 1 $aREIS JÚNIOR, O.
700 1 $aDOMINGUES, D. S.
700 1 $aSANTOS, T. B. dos
700 1 $aOLIVEIRA, F. F. de
700 1 $aPOT, D.
700 1 $aLEROY, T.
700 1 $aVIEIRA, L. G. E.
700 1 $aCARAZZOLLE, M. F.
700 1 $aPEREIRA, G. A. G.
700 1 $aPEREIRA, L. F. P.
773 $tPLOS ONE, January 2017.
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Registro original: |
Embrapa Café (CNPCa) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Solos. Para informações adicionais entre em contato com cnps.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
19/03/2010 |
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Data da última atualização: |
19/03/2010 |
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Tipo da produção científica: |
Monitoramento/Zoneamento |
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Autoria: |
ACCIOLY, L. J. de O.; SILVA, A. B. da; SOUSA, A. R. de; SILVA, E. A. da; ALVES, E. da S.; SILVA, J. A. da; LOPES, H. L. |
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Afiliação: |
LUCIANO JOSE DE OLIVEIRA ACCIOLY, CNPS; ADEMAR BARROS DA SILVA, CNPS; ANTONIO RAIMUNDO DE SOUSA, IPA; EDUARDO ALVES DA SILVA; EUDMAR DA SILVA ALVES; JULIANA ALVES DA SILVA; HELIO LEANDRO LOPES. |
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Título: |
Mapa de erosão rea: Rio Ipojuca. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
ACCIOLY, L. J. de O.; SILVA, A. B. da; SOUSA, A. R. Projeto Embrapa/Promata: Análise multicritério para avaliação de riscos de erosão e aptidão para cana-de-açúcar na zona da mata sul de Pernambuco - folhas (1:25.000) de Rio Ipojuca, Primavera e Escada. Rio de Janeiro: Embrapa Solos; Recife: Embrapa Solos UEP, 2009. 18 p. Rio de Janeiro: Embrapa Solos; Recife: Embrapa Solos UEP, 2009. |
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Páginas: |
1 p. |
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Descrição Física: |
1 mapa color.; escala 1:25.000. |
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Idioma: |
Português |
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Palavras-Chave: |
Mapa de erosão. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00895nem a2200193 a 4500
001 1661850
005 2010-03-19
008 2009 bl uuuu u0uu1 u #d
100 1 $aACCIOLY, L. J. de O.
245 $aMapa de erosão rea$bRio Ipojuca.
260 $aACCIOLY, L. J. de O.; SILVA, A. B. da; SOUSA, A. R. Projeto Embrapa/Promata: Análise multicritério para avaliação de riscos de erosão e aptidão para cana-de-açúcar na zona da mata sul de Pernambuco - folhas (1:25.000) de Rio Ipojuca, Primavera e Escada. Rio de Janeiro: Embrapa Solos; Recife: Embrapa Solos UEP, 2009. 18 p. Rio de Janeiro: Embrapa Solos; Recife: Embrapa Solos UEP$c2009
300 $a1 p.$c1 mapa color.; escala 1:25.000.
653 $aMapa de erosão
700 1 $aSILVA, A. B. da
700 1 $aSOUSA, A. R. de
700 1 $aSILVA, E. A. da
700 1 $aALVES, E. da S.
700 1 $aSILVA, J. A. da
700 1 $aLOPES, H. L.
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Embrapa Solos (CNPS) |
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