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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
30/01/2017 |
Data da última atualização: |
02/03/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FERREIRA, R. C. U.; CANÇADO, L. J.; VALLE, C. B. do; CHIARI, L.; SOUZA, A. P. de. |
Afiliação: |
REBECCA, C. U. FERREIRA, UNICAMP; LETÍCIA J. CANÇADO; CACILDA BORGES DO VALLE, CNPGC; LUCIMARA CHIARI, CNPGC; ANETE P DE SOUZA, UNICAMP. |
Título: |
Microsatellite loci for Urochloa decumbens (Stapf) R. D. Webster and cross-amplification in other Urochloa species. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
BMC Research Notes, v. 9, n. 152, p. 1-11, 2016. |
Idioma: |
Português |
Conteúdo: |
Background: Forage grasses of the African genus Urochloa (syn. Brachiaria) are the basis of Brazilian beef production, and there is a strong demand for high quality, productive and adapted forage plants. Among the approximately 100 species of the genus Urochloa, Urochloa decumbens is one of the most important tropical forage grasses used for pastures due to several of its agronomic attributes. However, the level of understanding of these attributes and the tools with which to control them at the genetic level are limited, mainly due to the apomixis and ploidy level of this species. In this context, the present study aimed to identify and characterize molecular microsatellite markers of U. decumbens and to evaluate their cross-amplification in other Urochloa species. Findings: Microsatellite loci were isolated from a previously constructed enriched library from one U. decumbens genotype. Specific primers were designed for one hundred thirteen loci, and ninety-three primer pairs successfully amplified microsatellite regions, yielding an average of 4.93 alleles per locus. The polymorphism information content ( IC) values of these loci ranged from 0.26 to 0.85 (average 0.68), and the associated discriminating power (DP) values ranged from 0.22 to 0.97 (average 0.77). Cross-amplification studies demonstrated the potential transferability of these microsatellites to four other Urochloa species. Structure analysis revealed the existence of three distinct groups, providing evidence in the allelic pool that U. decumbens is closely related to Urochloa ruziziensis and Urochloa brizantha. The genetic distance values determined using Jaccard?s coefficient ranged from 0.06 to 0.76. Conclusions: The microsatellite markers identified in this study are the first set of molecular markers for U. decumbens species. Their availability will facilitate understanding the genetics of this and other Urochloa species and breeding them, and will be useful for germplasm characterization, linkage mapping and marker-assisted selection. Keywords: Enriched library, Forage, Signalgrass, Simple sequence repeat, Transferability MenosBackground: Forage grasses of the African genus Urochloa (syn. Brachiaria) are the basis of Brazilian beef production, and there is a strong demand for high quality, productive and adapted forage plants. Among the approximately 100 species of the genus Urochloa, Urochloa decumbens is one of the most important tropical forage grasses used for pastures due to several of its agronomic attributes. However, the level of understanding of these attributes and the tools with which to control them at the genetic level are limited, mainly due to the apomixis and ploidy level of this species. In this context, the present study aimed to identify and characterize molecular microsatellite markers of U. decumbens and to evaluate their cross-amplification in other Urochloa species. Findings: Microsatellite loci were isolated from a previously constructed enriched library from one U. decumbens genotype. Specific primers were designed for one hundred thirteen loci, and ninety-three primer pairs successfully amplified microsatellite regions, yielding an average of 4.93 alleles per locus. The polymorphism information content ( IC) values of these loci ranged from 0.26 to 0.85 (average 0.68), and the associated discriminating power (DP) values ranged from 0.22 to 0.97 (average 0.77). Cross-amplification studies demonstrated the potential transferability of these microsatellites to four other Urochloa species. Structure analysis revealed the existence of three distinct groups, providing evidence ... Mostrar Tudo |
Palavras-Chave: |
Enriched library; Transferability. |
Thesaurus Nal: |
Forage; Microsatellite repeats; Urochloa brizantha. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154227/1/Microsatellite-loci-for-Urochloa.pdf
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Marc: |
LEADER 02829naa a2200229 a 4500 001 2062128 005 2017-03-02 008 2016 bl uuuu u00u1 u #d 100 1 $aFERREIRA, R. C. U. 245 $aMicrosatellite loci for Urochloa decumbens (Stapf) R. D. Webster and cross-amplification in other Urochloa species.$h[electronic resource] 260 $c2016 520 $aBackground: Forage grasses of the African genus Urochloa (syn. Brachiaria) are the basis of Brazilian beef production, and there is a strong demand for high quality, productive and adapted forage plants. Among the approximately 100 species of the genus Urochloa, Urochloa decumbens is one of the most important tropical forage grasses used for pastures due to several of its agronomic attributes. However, the level of understanding of these attributes and the tools with which to control them at the genetic level are limited, mainly due to the apomixis and ploidy level of this species. In this context, the present study aimed to identify and characterize molecular microsatellite markers of U. decumbens and to evaluate their cross-amplification in other Urochloa species. Findings: Microsatellite loci were isolated from a previously constructed enriched library from one U. decumbens genotype. Specific primers were designed for one hundred thirteen loci, and ninety-three primer pairs successfully amplified microsatellite regions, yielding an average of 4.93 alleles per locus. The polymorphism information content ( IC) values of these loci ranged from 0.26 to 0.85 (average 0.68), and the associated discriminating power (DP) values ranged from 0.22 to 0.97 (average 0.77). Cross-amplification studies demonstrated the potential transferability of these microsatellites to four other Urochloa species. Structure analysis revealed the existence of three distinct groups, providing evidence in the allelic pool that U. decumbens is closely related to Urochloa ruziziensis and Urochloa brizantha. The genetic distance values determined using Jaccard?s coefficient ranged from 0.06 to 0.76. Conclusions: The microsatellite markers identified in this study are the first set of molecular markers for U. decumbens species. Their availability will facilitate understanding the genetics of this and other Urochloa species and breeding them, and will be useful for germplasm characterization, linkage mapping and marker-assisted selection. Keywords: Enriched library, Forage, Signalgrass, Simple sequence repeat, Transferability 650 $aForage 650 $aMicrosatellite repeats 650 $aUrochloa brizantha 653 $aEnriched library 653 $aTransferability 700 1 $aCANÇADO, L. J. 700 1 $aVALLE, C. B. do 700 1 $aCHIARI, L. 700 1 $aSOUZA, A. P. de 773 $tBMC Research Notes$gv. 9, n. 152, p. 1-11, 2016.
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1. | | GOMES, T. C.; CUSTÓDIO, I. G.; GONÇALVES, D. L.; KARAM, D.; DIAS, L. L. C. Alelopatia de extratos de raízes de sorgo em soja, Brachiaria decumbens e picão-preto (Bidens subalternans). In: CONGRESSO NACIONAL DE MILHO E SORGO, 32., 2018, Lavras. Soluções integradas para os sistemas de produção de milho e sorgo no Brasil: resumos. Sete Lagoas: Associação Brasileira de Milho e Sorgo, 2018. p. 338.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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4. | | GOMES, T. C.; CUSTÓDIO, I. G.; PADRÃO, V. A.; KARAM, D.; DIAS, L. L. C.; SIMEONE, M. L. F. Quantificação e perfil de extratos de raízes sorgo. In: CONGRESSO NACIONAL DE MILHO E SORGO, 32., 2018, Lavras. Soluções integradas para os sistemas de produção de milho e sorgo no Brasil: resumos. Sete Lagoas: Associação Brasileira de Milho e Sorgo, 2018. p. 349.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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5. | | MARIZ, B. L.; GUIMARAES, L. J. M.; JORGE, K.; DIAS, L. L. C.; QUEIROZ, V. A. V.; SILVA, D. D. da. Correlações entre caracteres de espigas e incidência de grãos ardidos e fumonisinas em grãos de milho. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA PIBIC/CNPq, 12., 2017, Sete Lagoas. [Trabalhos apresentados]. Sete Lagoas: Embrapa Milho e Sorgo, 2017.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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6. | | IAREMA, L.; CRUZ, A. C. F. da; SALDANHA, C. W.; DIAS, L. L. C.; VIEIRA, R. F.; OLIVEIRA, E. J. de; OTONI, W. C. Photoautotrophic propagation of Brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen]. Plant Cell Tissue and Organ Culture, v. 110, p. 227-238, 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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7. | | NODA, R. W.; LAAT, D. M. de; GUIMARÃES, C. T.; SOUZA, T. C. de; DIAS, L. L. C.; LOBO, F. P.; MAGALHÃES, J. V. de; COIMBRA, R. S.; MAGALHÃES, P. C.; ZERLOTINI, A.; FRANCO, G. M.; CARNEIRO, N. P. GO enrichment of microarray data from maize root under drough. In: ANNUAL INTERNATIONAL CONFERENCE ON INTELLIGENT SYSTEMS FOR MOLECULAR BIOLOGY, 21.; EUROPEAN CONFERENCE ON COMPUTATIONAL BIOLOGY, 12., 2013, Berlin. Posters... Berlin: ISCB, 2013. Pôster F53.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agricultura Digital; Embrapa Milho e Sorgo. |
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