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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
19/10/2023 |
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Data da última atualização: |
26/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, J. C. de; SILVA, A. L. D. da; SILVA, L. M. da; FORMIGHIERI, E. F.; PETERS, L. P.; ASSIS, G. M. L. de; SILVA, C. C. da; SOUZA, A. P. DE; CAMPOS, T. de. |
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Afiliação: |
JÔNATAS CHAGAS DE OLIVEIRA, UNIVERSIDADE FEDERAL DO ACRE; ANDRÉ LUCAS DOMINGOS DA SILVA, UNIVERSIDADE FEDERAL DO ACRE; LUCIELIO MANOEL DA SILVA, CPATC; EDUARDO FERNANDES FORMIGHIERI, CNPAE; LEILA PRISCILA PETERS, UNIVERSIDADE FEDERAL DO ACRE; GISELLE MARIANO LESSA DE ASSIS, CPAF-AC; CARLA CRISTINA DA SILVA, UNIVERSIDADE DE CAMPINAS; ANETE PEREIRA DE SOUZA, UNIVERSITY OF CAMPINAS; TATIANA DE CAMPOS, CPAF-AC. |
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Título: |
Novel microsatellite markers derived from Arachis pintoi transcriptome sequencing for cross-species transferability and varietal identification. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plant Molecular Biology Reporter, v. 41, n. 4, 2023. |
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DOI: |
https://doi.org/10.1007/s11105-023-01402-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Forage peanut (Arachis pintoi) is an important leguminous forage that has gained popularity due to increased livestock productivity. Furthermore, the species helps with soil fertility and the restoration of degraded areas. However, A. pintoi has a limited number of molecular markers. The objective of this study was to create and characterize gene-derived microsatellite markers as well as to test their transferability to the peanut (Arachis hypogaea) and other six wild Arachis species. A total of 4461 putative simple sequence repeats (SSR) were identified, and PCR primer pairs were designed for 999 SSR regions after filtering out primers with the same annealing site and searching for sequences related to open reading frames (ORFs). The dinucleotide motif was the most common (628; 62.86%). For validation, 186 primer pairs were chosen at random, of which 63 (33.87%) were polymorphic, with an average of 7.37 alleles per locus. Polymorphic information content (PIC = 0.70) and discriminatory power (D = 0.80) were both high on average. The functional annotation discovered 120 sequences that were assigned to 87 gene ontology functional groups divided into three main categories: molecular function (27 sub-categories), cellular components (21 sub-categories), and biological process (39 sub-categories). Thirty-three SSRs were tested for transferability to peanut and six other wild Arachis species, resulting in variable cross-species amplification (63.64 to 100%). Here, we present the first gene-derived SSR for A. pintoi. These new informative microsatellites may be linked to agronomically important genes to be used in genetic studies. MenosForage peanut (Arachis pintoi) is an important leguminous forage that has gained popularity due to increased livestock productivity. Furthermore, the species helps with soil fertility and the restoration of degraded areas. However, A. pintoi has a limited number of molecular markers. The objective of this study was to create and characterize gene-derived microsatellite markers as well as to test their transferability to the peanut (Arachis hypogaea) and other six wild Arachis species. A total of 4461 putative simple sequence repeats (SSR) were identified, and PCR primer pairs were designed for 999 SSR regions after filtering out primers with the same annealing site and searching for sequences related to open reading frames (ORFs). The dinucleotide motif was the most common (628; 62.86%). For validation, 186 primer pairs were chosen at random, of which 63 (33.87%) were polymorphic, with an average of 7.37 alleles per locus. Polymorphic information content (PIC = 0.70) and discriminatory power (D = 0.80) were both high on average. The functional annotation discovered 120 sequences that were assigned to 87 gene ontology functional groups divided into three main categories: molecular function (27 sub-categories), cellular components (21 sub-categories), and biological process (39 sub-categories). Thirty-three SSRs were tested for transferability to peanut and six other wild Arachis species, resulting in variable cross-species amplification (63.64 to 100%). Here, we present the f... Mostrar Tudo |
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Thesaurus Nal: |
Arachis; Forage; Peanuts. |
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Categoria do assunto: |
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Marc: |
LEADER 02495naa a2200265 a 4500
001 2157375
005 2024-02-26
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11105-023-01402-9$2DOI
100 1 $aOLIVEIRA, J. C. de
245 $aNovel microsatellite markers derived from Arachis pintoi transcriptome sequencing for cross-species transferability and varietal identification.$h[electronic resource]
260 $c2023
520 $aForage peanut (Arachis pintoi) is an important leguminous forage that has gained popularity due to increased livestock productivity. Furthermore, the species helps with soil fertility and the restoration of degraded areas. However, A. pintoi has a limited number of molecular markers. The objective of this study was to create and characterize gene-derived microsatellite markers as well as to test their transferability to the peanut (Arachis hypogaea) and other six wild Arachis species. A total of 4461 putative simple sequence repeats (SSR) were identified, and PCR primer pairs were designed for 999 SSR regions after filtering out primers with the same annealing site and searching for sequences related to open reading frames (ORFs). The dinucleotide motif was the most common (628; 62.86%). For validation, 186 primer pairs were chosen at random, of which 63 (33.87%) were polymorphic, with an average of 7.37 alleles per locus. Polymorphic information content (PIC = 0.70) and discriminatory power (D = 0.80) were both high on average. The functional annotation discovered 120 sequences that were assigned to 87 gene ontology functional groups divided into three main categories: molecular function (27 sub-categories), cellular components (21 sub-categories), and biological process (39 sub-categories). Thirty-three SSRs were tested for transferability to peanut and six other wild Arachis species, resulting in variable cross-species amplification (63.64 to 100%). Here, we present the first gene-derived SSR for A. pintoi. These new informative microsatellites may be linked to agronomically important genes to be used in genetic studies.
650 $aArachis
650 $aForage
650 $aPeanuts
700 1 $aSILVA, A. L. D. da
700 1 $aSILVA, L. M. da
700 1 $aFORMIGHIERI, E. F.
700 1 $aPETERS, L. P.
700 1 $aASSIS, G. M. L. de
700 1 $aSILVA, C. C. da
700 1 $aSOUZA, A. P. DE
700 1 $aCAMPOS, T. de
773 $tPlant Molecular Biology Reporter$gv. 41, n. 4, 2023.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
10/07/2013 |
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Data da última atualização: |
20/11/2013 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
RODRIGUES, D. de S.; MENDES, T. D.; PACHECO, T. F. |
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Afiliação: |
DASCIANA DE SOUSA RODRIGUES, CNPAE; THAIS DEMARCHI MENDES, CNPAE; THALYTA FRAGA PACHECO, CNPAE. |
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Título: |
Catálise enzimática para desconstrução de biomassa lignocelulósica. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: MACHADO, C. M. M. (Ed.). Microrganismos na produção de biocombustíveis líquidos. Brasília, DF: Embrapa Agroenergia, p. 145-187, 2013. |
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Idioma: |
Português |
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Conteúdo: |
Introdução; estrutura da biomassa lignocelulósica; estrutura e propriedade de enzimas que degradam materiais lignocelulósicos; função de diferentes enzimas na desconstrução de biomassa lignocelulósicas; modo de ação de enzimas que degradam biomassa; desafios na hidrólise enzimática de biomassa; avanços no processo enzimático de desconstrução de biomassa; considerações finais. |
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Palavras-Chave: |
Catálise enzimática; Lignocelulósica. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00973naa a2200169 a 4500
001 1961748
005 2013-11-20
008 2013 bl uuuu u00u1 u #d
100 1 $aRODRIGUES, D. de S.
245 $aCatálise enzimática para desconstrução de biomassa lignocelulósica.
260 $c2013
520 $aIntrodução; estrutura da biomassa lignocelulósica; estrutura e propriedade de enzimas que degradam materiais lignocelulósicos; função de diferentes enzimas na desconstrução de biomassa lignocelulósicas; modo de ação de enzimas que degradam biomassa; desafios na hidrólise enzimática de biomassa; avanços no processo enzimático de desconstrução de biomassa; considerações finais.
653 $aCatálise enzimática
653 $aLignocelulósica
700 1 $aMENDES, T. D.
700 1 $aPACHECO, T. F.
773 $tIn: MACHADO, C. M. M. (Ed.). Microrganismos na produção de biocombustíveis líquidos. Brasília, DF: Embrapa Agroenergia, p. 145-187, 2013.
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