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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
19/09/2023 |
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Data da última atualização: |
19/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MENEZES, V. G.; MONTE, A. P. O.; BARBERINO, R. de S.; GOUVEIA, B. B.; LINS, T. L. B.; MACEDO, T. J. S. e; OLIVEIRA JÚNIOR, J. L. de; ARAÚJO, C. de A.; CAMPOS, F. S.; OLIVEIRA FILHO, E. F. de; BATISTA, A. M.; QUEIROZ, M. A. A.; ARAUJO, G. G. L. de; MATOS, M. H. T. de; WISCHRA, A. |
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Afiliação: |
VANÚZIA GONÇALVES MENEZES, UNIVASF; ALANE PAINS OLIVEIRA DO MONTE, UNIVASF; RICÁSSIO DE SOUSA BARBERINO, UNIVASF; BRUNA BORTOLONI GOUVEIA, UNIVASF; THAE LANE BARBOSA GAMA LINS, UNIVASF; TAÍS JOBARD SILVA E MACEDO, UNIVASF; JOÃOZITO LIANDRO DE OLIVEIRA JÚNIOR, UNIVASF; CLEYTON DE ALMEIDA ARAÚJO, Universidade Federal do Agreste Pernambucano, Garanhuns, PE; FLEMING SENA CAMPOS, Universidade Federal do Maranh ̃ao - UFMA, Chapadina, MA; EMANUEL FELIPE DE OLIVEIRA FILHO, UFRPE; ANDRÉ MARIANO BATISTA, UFRPE; MARIO ADRIANO ÁVILA QUEIROZ, UNIVASF; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; MARIA HELENA TAVARES DE MATOS, UNIVASF; AUREA WISCHRA, UFRPE. |
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Título: |
Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 255, 107278, Jun. 2023. |
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DOI: |
https://doi.org/10.1016/j.anireprosci.2023.107278 |
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Idioma: |
Inglês |
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Conteúdo: |
This study evaluated the effect of reduced water intake on survival, apoptosis and immunoexpression of leptin in sheep preantral follicles, activation of primordial follicles, serum levels of leptin, estradiol (E2) and progesterone (P4), and in vitro maturation (IVM) of oocytes antral follicles, as well evaluated the effects of leptin on in vitro culture of secondary follicles isolated these animals. Ewes (n = 32) were divided into four groups: water ad libitum (Control ? 100%), 80%; 60% and 40% of ad libitum intake. Blood was collected to determine, leptin, E2 and P4, before and after experiment. After the slaughter, ovarian cortex was used to histological and immunohistochemistry analysis and oocytes IVM. Moreover, isolated secondary follicles were cultured in vitro for 12 days in control medium (α-MEM+) or α-MEM+ with 10 or 25 ng/mL leptin. The reduction of water intake caused a linear decreasing effect on the percentages of normal preantral follicles, especially of primordial (P < 0.05), increased the apoptosis (P < 0.05) and decreased leptin expression in preantral follicles. The treatment with 60% of water intake showed greater total growth rate of isolated secondary follicles cultured with 25 ng/L leptin (P < 0.05), compared to those cultured in α-MEM+ . In conclusion, reduced water intake impaired the number of normal sheep preantral follicles, especially of primordial follicles, increased apoptosis and decreased leptin expression in preantral follicles. Moreover, secondary follicles from of ewes MenosThis study evaluated the effect of reduced water intake on survival, apoptosis and immunoexpression of leptin in sheep preantral follicles, activation of primordial follicles, serum levels of leptin, estradiol (E2) and progesterone (P4), and in vitro maturation (IVM) of oocytes antral follicles, as well evaluated the effects of leptin on in vitro culture of secondary follicles isolated these animals. Ewes (n = 32) were divided into four groups: water ad libitum (Control ? 100%), 80%; 60% and 40% of ad libitum intake. Blood was collected to determine, leptin, E2 and P4, before and after experiment. After the slaughter, ovarian cortex was used to histological and immunohistochemistry analysis and oocytes IVM. Moreover, isolated secondary follicles were cultured in vitro for 12 days in control medium (α-MEM+) or α-MEM+ with 10 or 25 ng/mL leptin. The reduction of water intake caused a linear decreasing effect on the percentages of normal preantral follicles, especially of primordial (P < 0.05), increased the apoptosis (P < 0.05) and decreased leptin expression in preantral follicles. The treatment with 60% of water intake showed greater total growth rate of isolated secondary follicles cultured with 25 ng/L leptin (P < 0.05), compared to those cultured in α-MEM+ . In conclusion, reduced water intake impaired the number of normal sheep preantral follicles, especially of primordial follicles, increased apoptosis and decreased leptin expression in preantral follicle... Mostrar Tudo |
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Palavras-Chave: |
Foliculogênese; Folículos primordiais; Hormônios sexuais; Oócito. |
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Thesagro: |
Água Potável; Ovelha. |
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Thesaurus Nal: |
Drinking water; Oocytes; Sex hormones; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156781/1/Effect-of-reduced-water-intake-on-ovarian-reserve-leptin-Ovelha-Foliculo-2023.pdf
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Marc: |
LEADER 02840naa a2200421 a 4500
001 2156781
005 2023-09-19
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.anireprosci.2023.107278$2DOI
100 1 $aMENEZES, V. G.
245 $aEffect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles.$h[electronic resource]
260 $c2023
520 $aThis study evaluated the effect of reduced water intake on survival, apoptosis and immunoexpression of leptin in sheep preantral follicles, activation of primordial follicles, serum levels of leptin, estradiol (E2) and progesterone (P4), and in vitro maturation (IVM) of oocytes antral follicles, as well evaluated the effects of leptin on in vitro culture of secondary follicles isolated these animals. Ewes (n = 32) were divided into four groups: water ad libitum (Control ? 100%), 80%; 60% and 40% of ad libitum intake. Blood was collected to determine, leptin, E2 and P4, before and after experiment. After the slaughter, ovarian cortex was used to histological and immunohistochemistry analysis and oocytes IVM. Moreover, isolated secondary follicles were cultured in vitro for 12 days in control medium (α-MEM+) or α-MEM+ with 10 or 25 ng/mL leptin. The reduction of water intake caused a linear decreasing effect on the percentages of normal preantral follicles, especially of primordial (P < 0.05), increased the apoptosis (P < 0.05) and decreased leptin expression in preantral follicles. The treatment with 60% of water intake showed greater total growth rate of isolated secondary follicles cultured with 25 ng/L leptin (P < 0.05), compared to those cultured in α-MEM+ . In conclusion, reduced water intake impaired the number of normal sheep preantral follicles, especially of primordial follicles, increased apoptosis and decreased leptin expression in preantral follicles. Moreover, secondary follicles from of ewes
650 $aDrinking water
650 $aOocytes
650 $aSex hormones
650 $aSheep
650 $aÁgua Potável
650 $aOvelha
653 $aFoliculogênese
653 $aFolículos primordiais
653 $aHormônios sexuais
653 $aOócito
700 1 $aMONTE, A. P. O.
700 1 $aBARBERINO, R. de S.
700 1 $aGOUVEIA, B. B.
700 1 $aLINS, T. L. B.
700 1 $aMACEDO, T. J. S. e
700 1 $aOLIVEIRA JÚNIOR, J. L. de
700 1 $aARAÚJO, C. de A.
700 1 $aCAMPOS, F. S.
700 1 $aOLIVEIRA FILHO, E. F. de
700 1 $aBATISTA, A. M.
700 1 $aQUEIROZ, M. A. A.
700 1 $aARAUJO, G. G. L. de
700 1 $aMATOS, M. H. T. de
700 1 $aWISCHRA, A.
773 $tAnimal Reproduction Science$gv. 255, 107278, Jun. 2023.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Algodão. Para informações adicionais entre em contato com cnpa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
01/09/1993 |
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Data da última atualização: |
01/09/1993 |
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Autoria: |
STERLING, W. L. |
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Título: |
Relatorio final da consultoria sobre o manejo integrado de pragas do algodoeiro. |
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Ano de publicação: |
1980 |
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Fonte/Imprenta: |
Campina Grande: EMBRAPA-CNPA, 1980 |
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Páginas: |
23p |
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Idioma: |
Inglês |
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Palavras-Chave: |
Management; Pest; Relatorio; Report. |
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Thesagro: |
Algodão; Gossypium Hirsutum; Manejo; Praga. |
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Thesaurus NAL: |
cotton. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00547nam a2200217 a 4500
001 1262867
005 1993-09-01
008 1980 bl uuuu u0uu1 u #d
100 1 $aSTERLING, W. L.
245 $aRelatorio final da consultoria sobre o manejo integrado de pragas do algodoeiro.
260 $aCampina Grande: EMBRAPA-CNPA$c1980
300 $a23p
650 $acotton
650 $aAlgodão
650 $aGossypium Hirsutum
650 $aManejo
650 $aPraga
653 $aManagement
653 $aPest
653 $aRelatorio
653 $aReport
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Embrapa Algodão (CNPA) |
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