Data/hora: 14/06/2024 16:39:00

Biblioteca(s): 	Embrapa Agricultura Digital; Embrapa Milho e Sorgo.	
Data corrente: 	10/07/2017	
Data da última atualização: 	24/01/2018	
Autoria: 	RIBEIRO, A. P.; SOUZA, W. R. de; MARTINS, P. K.; VINECKY, F.; DUARTE, K. E.; BASSO, M. F.; DIAS, B. B. A.; CAMPANHA, R. B.; OLIVEIRA, P. A. de; CENTENO, D. C.; CANÇADO, G. M. de A.; MAGALHÃES, J. V. de; SOUSA, C. A. F. de; ANDRADE, A. C.; KOBAYASHI, A. K.; MOLINARI, H. B. C.	
Afiliação: 	WAGNER R. DE SOUZA, UFLA; POLYANA K. MARTINS, UFLA; FELIPE VINECKY, UFLA; KAROLINE E. DUARTE, UFLA; MARCOS F. BASSO, UFLA; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; RAQUEL BOMBARDA CAMPANHA, CNPAE; PATRICIA ABRAO DE OLIVEIRA, CNPAE; DANILO C. CENTENO, UFABC; GERALDO MAGELA DE ALMEIDA CANCADO, CNPTIA; JURANDIR VIEIRA DE MAGALHAES, CNPMS; CARLOS A. F. DE SOUSA, UFLA; ALAN CARVALHO ANDRADE, SAPC; ADILSON KENJI KOBAYASHI, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE.	
Título: 	Overexpression of BdMATE gene improves aluminum tolerance in Setaria viridis.	
Ano de publicação: 	2017	
Fonte/Imprenta: 	Frontiers in Plant Science, v. 8, p. 1-12, June 2017.	
Páginas: 	12 p.	
DOI: 	10.3389/fpls.2017.00865	
Idioma: 	Inglês	
Conteúdo: 	Acidic soils are distributed worldwide, predominantly in tropical and subtropical areas,reaching around 50% of the arable soil. This type of soil strongly reduces crop production, mainly because of the presence of aluminum, which has its solubility increased at low pH levels. A well-known physiological mechanism used by plants to cope with Al stress involves activation of membrane transporters responsible for organic acid anions secretion from the root apex to the rhizosphere, which chelate Al, preventing its absorption by roots. In sorghum, a membrane transporter gene belonging to multidrug and toxic compound extrusion (MATE) family was identified and characterized as an aluminum-activated citrate transporter gene responsible for Al tolerance in this crop. Setaria viridis is an emerging model for C4 species and it is an important model to validate some genes for further C4 crops transformation, such as sugarcane, maize, and wheat. In the present work, Setaria viridis was used as a model plant to overexpress a newly identified MATE gene from Brachypodium distachyon(BdMATE), closely related to SbMATE, for aluminum tolerance assays. Transgenic S. viridis plants overexpressing a BdMATE presented an improved Al tolerance phenotype, characterized by sustained root growth and exclusion of aluminum from the root apex in transgenic plants, as confirmed by hematoxylin assay. In addition, transgenic plants showed higher root citrate exudation into the rhizosphere, suggesting that Al tolerance improvement in these plants could be related to the chelation of the metal by the organic acid anion. These results suggest that BdMATE gene can be used to transform C4 crops of economic importance with improved aluminum tolerance.	
Palavras-Chave: 	BdMate; Hydroponic system; Organismo geneticamente modificado; Tolerância ao alumínio.	
Thesagro: 	Aluminio.	
Thesaurus Nal: 	Abiotic stress; Aluminum; Genetically modified organisms; Setaria viridis.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
Registro original: 	Embrapa Milho e Sorgo (CNPMS)	
Exemplares: 	CNPTIA - (19234-1) - UPC - AP - DD -  -  -  - DIS
CNPMS - (27962-1) - UPC - AP - DD -  -  -  - DIS