Data/hora: 03/06/2024 10:50:48

Biblioteca(s): | Embrapa Agroenergia.
Data corrente: | 01/10/2015
Data da última atualização: | 20/09/2017
Autoria: | GUERRERO, E. B.; ARNEODO, J.; CAMPANHA, R. B.; OLIVEIRA, P. A. de; LABARTE, M. T. V.; CATALDI, T. R.; CAMPOS, E.; CATALDI, A.; LABARTE, C. A.; RODRIGUES, C. M.; TALIA, P.
Afiliação: | Emiliano Ben Guerrero, INTA; Joel Arneodo, CONICET; RAQUEL BOMBARDA CAMPANHA, CNPAE; PATRICIA ABRAO DE OLIVEIRA, CNPAE; Mônica T. Veneziano Labate, USP; Thaís Regiani Cataldi, USP; Eleonora Campos, INTA; Angel Cataldi, INTA; Carlos A. Labate, USP; CLENILSON MARTINS RODRIGUES, CNPAE; Paola Talia, INTA.
Título: | Prospection and evaluation of (Hemi) cellulolytic enzymes using untreated and pretreated biomasses in two argentinean native termites.
Ano de publicação: | 2015
Fonte/Imprenta: | PLoS ONE, v. 10, n. 8, p1-23. 23p, 2015
DOI: | 10.1371/journal.pone.0136573
Idioma: | Inglês
Conteúdo: | Saccharum officinarum bagasse (common name: sugarcane bagasse) and Pennisetum purpureum (also known as Napier grass) are among the most promising feedstocks for bioethanol production in Argentina and Brazil. In this study, both biomasses were assessed before and after acid pretreatment and following hydrolysis with Nasutitermes aquilinus and Cortaritermes fulviceps termite gut digestome. The chemical composition analysis of the biomasses after diluted acid pretreatment showed that the hemicellulose fraction was partially removed. The (hemi) cellulolytic activities were evaluated in bacterial culture supernatants of termite gut homogenates grown in treated and untreated biomasses. In all cases, we detected significantly higher endoglucanase and xylanase activities using pretreated biomasses compared to untreated biomasses, carboxymethylcellulose and xylan. Several protein bands with (hemi) cellulolytic activity were detected in zymograms and two-dimensional gel electrophoresis. Some proteins of these bands or spots were identified as xylanolytic peptides by mass spectrometry. Finally, the diversity of cultured cellulolytic bacterial endosymbionts associated to both Argentinean native termite species was analyzed. This study describes, for the first time, bacterial endosymbionts and endogenous (hemi) cellulases of two Argentinean native termites as well as their potential application in degradation of lignocellulosic biomass for bioethanol production.
Palavras-Chave: | Bioetanol; Subproduto da cana de açúcar.
Thesagro: | Pennisetum Purpureum; Saccharum Officinarum.
Thesaurus Nal: | bioethanol.
Categoria do assunto: | --
Registro original: | Embrapa Agroenergia (CNPAE)
Exemplares: | CNPAE - (2711-1) - UPC - AP - DD -  -  -  - DIS