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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
29/03/2019 |
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Data da última atualização: |
21/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMARGO, L. S. de A.; MUNK, M.; SALES, J. N.; WOHLRES-VIANA, S.; QUINTAO, C. C. R.; VIANA, J. H. M. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MICHELE MUNK, UFJF; JOSE NELIO SALES, UFLA; SABINE WOHLRES-VIANA, UFJF; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
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Título: |
Differential gene expression between in vivo and in vitro maturation: a comparative study with bovine oocytes derived from the same donor pool. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
JBRA Assisted Reproduction, v. 23, n. 1, p. 7-14, 2019. |
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DOI: |
10.5935/1518-0557.20180084 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Luiz Sergio Almeida Camargo. |
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Conteúdo: |
Objective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p<0.05) abundance of peroxiredoxin 1 (Prdx1), heat shock protein 70.1 (Hsp70.1), growth and differentiation factor 9 (Gdf9), and maternal antigen that embryo requires (Mater) transcripts than the oocytes in the IN VIVO-OPU group, all obtained from the same pool of donor cows. Similar results were seen in the comparisons involving the IN VIVO-OPU and IN VITRO-Abattoir groups (p<0.05). Conclusion: In vitro maturation affected the abundance of polyadenylated transcripts in the oocyte cytoplasm when compared to in vivo maturation induced by exogenous hormones in oocytes collected from the same donor pool. MenosObjective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p<0.05) abundance of peroxiredoxin 1 (Prdx1), heat shock protein 70.1 (Hsp70.1), growth and differentiation factor 9 (Gdf9), and maternal antigen that embryo requires (Mater) transcripts than the oocytes in the IN VIVO-OPU group, all obtained from the same pool of donor cows. Similar results were seen in the comparisons involving the IN VIVO-OPU and IN VITRO-Abattoir groups (p<0.05). Conclusion: In vitro maturation affected the abundance of p... Mostrar Tudo |
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Palavras-Chave: |
In vivo maturation; MRNA; Ovum pick-up. |
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Thesaurus Nal: |
Gene expression. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/195183/1/jbra-23-01-0007.pdf
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Marc: |
LEADER 02498naa a2200253 a 4500
001 2107759
005 2022-06-21
008 2019 bl uuuu u00u1 u #d
024 7 $a10.5935/1518-0557.20180084$2DOI
100 1 $aCAMARGO, L. S. de A.
245 $aDifferential gene expression between in vivo and in vitro maturation$ba comparative study with bovine oocytes derived from the same donor pool.$h[electronic resource]
260 $c2019
500 $aNa publicação: Luiz Sergio Almeida Camargo.
520 $aObjective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p<0.05) abundance of peroxiredoxin 1 (Prdx1), heat shock protein 70.1 (Hsp70.1), growth and differentiation factor 9 (Gdf9), and maternal antigen that embryo requires (Mater) transcripts than the oocytes in the IN VIVO-OPU group, all obtained from the same pool of donor cows. Similar results were seen in the comparisons involving the IN VIVO-OPU and IN VITRO-Abattoir groups (p<0.05). Conclusion: In vitro maturation affected the abundance of polyadenylated transcripts in the oocyte cytoplasm when compared to in vivo maturation induced by exogenous hormones in oocytes collected from the same donor pool.
650 $aGene expression
653 $aIn vivo maturation
653 $aMRNA
653 $aOvum pick-up
700 1 $aMUNK, M.
700 1 $aSALES, J. N.
700 1 $aWOHLRES-VIANA, S.
700 1 $aQUINTAO, C. C. R.
700 1 $aVIANA, J. H. M.
773 $tJBRA Assisted Reproduction$gv. 23, n. 1, p. 7-14, 2019.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Biblioteca |
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| Registros recuperados : 304 | |
| 121. |  | ARAUJO, T. D.; JASMIN, J.; QUINTAO, C. C. R.; SOUZA, E. D.; VIANA, J. H. M.; CAMARGO, L. S. de A. Effect of DNA methylation inhibitor on heterochromatin in bovine embryos derived from heat-shocked oocytes In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 2016, Louisville. Reproduction, fertility and development: proceedings. Louisville: [s.n.], 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 123. | | VIEIRA, A. C.; QUINTAO, C. C. R.; SIQUEIRA, L. G. B.; OLIVEIRA, C. S.; SILVA, T. A.; VIEIRA, C. D.; CAMARGO, L. S. de A. Effect of epigenetic modulators on development of bovine embryos derived from heatshocked oocytes. Animal Reproduction, v. 15, n. 3, p. 483, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 124. | | QUINTAO, C. C. R.; SOUZA, E. D.; OLIVEIRA, C. S.; FREITAS, C. de; OLIVEIRA, J. A. S.; CAMARGO, L. S. de A. Effect of handmade biopsy on bovine embryons at the sixth day post in vitro fertilization Animal Reproduction., v.12, n.3, Jul./Sept. 2015. Edição dos proceedings do 29º Annual Meeting of the Brazilian Embryo Technology Society, 2015, Gramado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 126. | | COSTA, F. Q.; PEREIRA, M. M.; WOHLRES VIANA, S.; SERAPIAO, R. V.; CARVALHO, B. C.; VIANA, J. H. M.; NOGUEIRA, L. A. G.; CAMARGO, L. S. de A. Effect of heat stress during oocyte maturation on gene expression of in vitro-fertilized and parthenogenetic bovine blastocysts. In: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 127. | | ASCARI, I. J.; ALVES, N. G.; CAMARGO, L. S. de A.; JASMIN, J.; QUINTAO, C. C. R.; OLIVEIRA, J. A. S.; ARAÚJO, T. D. Effect of melatonin on DNA fragmentation and in vitro maturation of bovine oocytes subjected to heat shock Animal Reproduction., v.12, n.3, Jul./Sept. 2015. Edição dos proceedings do 29º Annual Meeting of the Brazilian Embryo Technology Society, 2015, Gramado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 128. | | PEREIRA, M. M.; MACHADO, M. A.; COSTA, F. de Q.; SERAPIÃO, R. V.; VIANA, J. H. M.; CAMARGO, L. S. de A. Effect of oxygen tension and serum during IVM on developmental competence of bovine oocytes. Reproduction, Fertility and Development, v. 22, p. 1074-1082, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 129. | | CAMARGO, L. S. de A.; SIQUEIRA, L. G. B.; SARAIVA, N. Z.; MAIA, G.; NOGUEIRA, B.; QUINTAO, C. C. R.; OLIVEIRA, C. S. Effect of physiological heat stress on HSP70 and CASPASE 3 levels in bovine oocytes. In: ANNUAL CONFERENCE, 52., 2019, San Jose. Beyond possible: remarkable transformation of reproductive biology: abstracts. San Jose: Society for the Study of Reproduction, 2019.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 130. | | VIANA, J. H. M.; VARGAS, M. S. B.; SIQUEIRA, L. G. B.; CAMARGO, L. S. de A.; FIGUEIREDO, A. C. S.; FERNANDES, C. A. C.; PALHÃO, M. P. Efficacy of induction of luteolysis in superovulated cows is dependent on time of prostaglandin F2alpha analog treatment: effects on plasma progesterone and luteinizing hormone profiles. Theriogenology, v. 86, n. 4, p. 934-939, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| 131. | | WOHLERS-VIANA, S.; BOITE, M. M.; PEREIRA, M. M.; SA, W. F.; VIANA, J. H. M.; MACHADO, M. A.; CAMARGO, L. S. de A. Effect of production system and breed on relative expression in bovine embryos. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY - REPRODUCTION, FERTILITY AND DEVELOPMENT., 21., 2009, San Diego. Proceedings... San Diego: Csiro, 2009. 1| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 132. | | WOHLRES-VIANA, S.; BOITE, M. C.; PEREIRA, M. M.; SA, W. F. de; VIANA, J. H. M.; MACHADO, M. A.; CAMARGO, L. S. de A. Effect of production system and breed on relative expression in bovine embryos In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY - REPRODUCTION, FERTILITY AND DEVELOPMENT, 21., 2009, San Diego. Proceeding... San Diego: Csiro, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 133. | | PATROCÍNIOS. T. A.; FERNANDES, C. A. C.; AMORIM, L. S.; RIBEIRO. J. R.; MACEDO, G. C.; SILVA, A. D.; VIANA, J. H. M.; CAMARGO, L. S. de A. Effect of reservatrol analogue on development of in vitro-fertilized bovine embryos. Reproduction, Fertility and Development, v. 29, n. 1, p. 183, 2017. Edição dos proceedings da Annual Conference of the International Embryo Technology Society, Austin, 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 134. | | CAMARGO, L. S. de A.; FREITAS, C. de; SÁ, W. F. de; FERREIRA, A. de M.; SERAPIÃO, R. V.; VIANA, J. H. M. Gestation length, birth weigth and offspring gender ratio of in vitro-produced Gyr (Bos indicus) cattle embryos. Animal Reproduction Science, v. 120, n. 1/4, p. 10-15, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 135. | | FONSECA, J. F. da; BATISTA, R. I. T. P.; TREVIZAN, J. T.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; CAMARGO, L. S. de A. Goat incubator: can bovine oocytes be matured in the uterine horn of a goat? Semina: Ciências Agrárias, Londrina, v. 40, n. 6, supp. 3, p. 3789-3796, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 136. | | BATISTA, R. I. T. P.; CAMARGO, L. S. de A.; SOUZA FABJAN, J. M. G.; PRATES, J. F.; TREVIZAN, J. T.; BRANDÃO, F. Z.; FONSECA, J. F. da. Goat incubator: the doe as a life incubator of bovine oocytes - first step. Animal Reprodroduction, v. 14, n. 3, p. 738, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 137. | | CAMARGO, L. S. de A.; GONÇALVES, F. S.; QUINTAO, C. C. R.; IGUMA, L. T.; SOUZA, E. D.; SIQUEIRA, L. G. B.; VIANA, J. H. M. Genetic modification of bovine embryos by lentiviral vectors. Animal Reproduction, v.10, n.3, p. 359, Jul./Sept. 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 138. | | PEREIRA, M. M.; BRANDAO, H. de M.; RAPOSO, N. R.; SERAPIÃO, R. V.; CARVALHO, B. C.; CAMARGO, L. S. de A.; LADEIRA, L. O. Gene expression and apoptosis in bovine preimplantation embryo exposed to carbon nanotubes. In: INTERNATIONAL CONFERENCE ON FOOD AND AGRICULTURE APPLICATIONS OF NANOTECHNOLOGIES, 1., 2010, São Pedro, SP. [Anais...] São Pedro: Aptor Software, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 140. | | CARVALHEIRA, L. de R.; WENCESLAU, R. R.; RIBEIRO, L. dos S.; CARVALHO, B. C. de; BORGES, A. M.; CAMARGO, L. S. de A. Daily vaginal temperature in Girolando cows from three different genetic composition under natural heat stress. Translational Animal Science, v. 5, n. 3, txab138, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 304 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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