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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
26/12/2018 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OTERO, R.; HERNÁNDEZ, D.; CAMARGO, L. S. de A. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Production of bovine transgenic embryos by microinjection of a lentiviral vector in mature ovocytes. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Indian Journal of Science and Technology, v. 11, n. 31, 2018. |
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Páginas: |
8 p. |
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DOI: |
10.17485/ijst/2018/v11i31/130839 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Objective: To produce bovine transgenic embryos by microinjection of a lentiviral vector with the eGFP gene as a marker. Methods: Four treatments were designed: T1=Control: fertilized in vitro (FIV) with cumulus-oocyte complexes (CCOs), cultivated in CR2 medium with 10% FBS and incubated at 38.5°C in an atmosphere of 95% humidity and 5% CO₂. T2=Control of culture medium: CCOs removed by vortex in the presence of hyaluronidase, FIV, grown in SOF medium in hermetic bag, with a gaseous mixture of 5% CO₂, 5% O₂ and 90% N₂ and humidity saturated at 38.5°C. T3=Microinjection control: CCOs removed microinjected with TALP medium, FIV and cultured under the same treatment conditions T2. T4=Microinjected with the lentivirus: CCOs removed microinjected with the lentiviral vector and FIV and cultured in the same conditions of the T2 and T3 treatments. The rate of development of blastocysts at day eight and the expression of the eGFP gene were evaluated. Findings: No significant statistical differences were found (p> 0.05) in the production of blastocysts at day eight, between treatments T1, T2, and T3. The percentage of blastocysts found in the T4 treatment was significantly lower (p <0.05) than in the other treatments. All embryos obtained in T4 expressed the transgene of interest. Application / Improvements: It is concluded that the culture conditions used were adequate for T1, T2 and T3, added that the microinjection with the lentiviral vector influences in some way the embryonic development, although, the technique was highly efficient for obtaining transgenic embryos. MenosAbstract Objective: To produce bovine transgenic embryos by microinjection of a lentiviral vector with the eGFP gene as a marker. Methods: Four treatments were designed: T1=Control: fertilized in vitro (FIV) with cumulus-oocyte complexes (CCOs), cultivated in CR2 medium with 10% FBS and incubated at 38.5°C in an atmosphere of 95% humidity and 5% CO₂. T2=Control of culture medium: CCOs removed by vortex in the presence of hyaluronidase, FIV, grown in SOF medium in hermetic bag, with a gaseous mixture of 5% CO₂, 5% O₂ and 90% N₂ and humidity saturated at 38.5°C. T3=Microinjection control: CCOs removed microinjected with TALP medium, FIV and cultured under the same treatment conditions T2. T4=Microinjected with the lentivirus: CCOs removed microinjected with the lentiviral vector and FIV and cultured in the same conditions of the T2 and T3 treatments. The rate of development of blastocysts at day eight and the expression of the eGFP gene were evaluated. Findings: No significant statistical differences were found (p> 0.05) in the production of blastocysts at day eight, between treatments T1, T2, and T3. The percentage of blastocysts found in the T4 treatment was significantly lower (p <0.05) than in the other treatments. All embryos obtained in T4 expressed the transgene of interest. Application / Improvements: It is concluded that the culture conditions used were adequate for T1, T2 and T3, added that the microinjection with the lentiviral vector influen... Mostrar Tudo |
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Palavras-Chave: |
EGFP; Genetic Modification; Micromanipulation. |
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Thesaurus Nal: |
Green fluorescent protein. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/189326/1/Artigo-Indian-J-Sci-and-Tech-Camargo-Production.pdf
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Marc: |
LEADER 02307naa a2200217 a 4500
001 2102529
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
024 7 $a10.17485/ijst/2018/v11i31/130839$2DOI
100 1 $aOTERO, R.
245 $aProduction of bovine transgenic embryos by microinjection of a lentiviral vector in mature ovocytes.$h[electronic resource]
260 $c2018
300 $a8 p.
520 $aAbstract Objective: To produce bovine transgenic embryos by microinjection of a lentiviral vector with the eGFP gene as a marker. Methods: Four treatments were designed: T1=Control: fertilized in vitro (FIV) with cumulus-oocyte complexes (CCOs), cultivated in CR2 medium with 10% FBS and incubated at 38.5°C in an atmosphere of 95% humidity and 5% CO₂. T2=Control of culture medium: CCOs removed by vortex in the presence of hyaluronidase, FIV, grown in SOF medium in hermetic bag, with a gaseous mixture of 5% CO₂, 5% O₂ and 90% N₂ and humidity saturated at 38.5°C. T3=Microinjection control: CCOs removed microinjected with TALP medium, FIV and cultured under the same treatment conditions T2. T4=Microinjected with the lentivirus: CCOs removed microinjected with the lentiviral vector and FIV and cultured in the same conditions of the T2 and T3 treatments. The rate of development of blastocysts at day eight and the expression of the eGFP gene were evaluated. Findings: No significant statistical differences were found (p> 0.05) in the production of blastocysts at day eight, between treatments T1, T2, and T3. The percentage of blastocysts found in the T4 treatment was significantly lower (p <0.05) than in the other treatments. All embryos obtained in T4 expressed the transgene of interest. Application / Improvements: It is concluded that the culture conditions used were adequate for T1, T2 and T3, added that the microinjection with the lentiviral vector influences in some way the embryonic development, although, the technique was highly efficient for obtaining transgenic embryos.
650 $aGreen fluorescent protein
653 $aEGFP
653 $aGenetic Modification
653 $aMicromanipulation
700 1 $aHERNÁNDEZ, D.
700 1 $aCAMARGO, L. S. de A.
773 $tIndian Journal of Science and Technology$gv. 11, n. 31, 2018.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
10/07/2020 |
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Data da última atualização: |
09/11/2020 |
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Autoria: |
LOPES, M. A. |
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Afiliação: |
MAURICIO ANTONIO LOPES, CNPAE. |
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Título: |
Brasil precisa de estratégia nacional para a bioeconomia, diz Maurício Antônio Lopes. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Estado de S.Paulo, São Paulo, 24 jul. 2019. |
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Idioma: |
Português |
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Notas: |
Entrevista. |
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Conteúdo: |
O engenheiro agrônomo, pesquisador e ex-presidenta da Embrapa, Maurício Antônio Lopes, defende que o Brasil, país com a maior diversidade biológica do planeta, adote uma estratégia de longo prazo para a bioeconomia, área em que a nação pode se tornar uma potência global. |
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Palavras-Chave: |
Bioeconomia; Bioindústria. |
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Thesagro: |
Inovação; Nutrição Humana; Sistema de Alimentação. |
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Thesaurus NAL: |
Human nutrition. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/214569/1/Brasil-precisa-de-estrate769gia-nacional-para-a-bioeconomia-diz-Mauri769cio-Anto770nio-Lopes-2019.pdf
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Marc: |
LEADER 00864nam a2200193 a 4500
001 2123813
005 2020-11-09
008 2019 bl uuuu u00u1 u #d
100 1 $aLOPES, M. A.
245 $aBrasil precisa de estratégia nacional para a bioeconomia, diz Maurício Antônio Lopes.$h[electronic resource]
260 $aEstado de S.Paulo, São Paulo, 24 jul. 2019.$c2019
500 $aEntrevista.
520 $aO engenheiro agrônomo, pesquisador e ex-presidenta da Embrapa, Maurício Antônio Lopes, defende que o Brasil, país com a maior diversidade biológica do planeta, adote uma estratégia de longo prazo para a bioeconomia, área em que a nação pode se tornar uma potência global.
650 $aHuman nutrition
650 $aInovação
650 $aNutrição Humana
650 $aSistema de Alimentação
653 $aBioeconomia
653 $aBioindústria
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Embrapa Agroenergia (CNPAE) |
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