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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
15/06/2021 |
Data da última atualização: |
15/09/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SIMÕES, L. M. S.; SANTOS, A. P. C.; BOTTINO, M. P.; LIMA, E. A.; FERNANDES, U. R.; ORLANDI, R. E.; RODRIGUES, S. A. D.; CAIXETA, F. M.; ALVES, N. G.; SOUZA, J. C.; QUINTAO, C. C. R.; CAMARGO, L. S. A.; DODE, M. A. N.; SALES, J. N. S. |
Afiliação: |
L. M. S. SIMÕES, UFLA; A. P. C. SANTOS, UFLA; M. P. BOTTINO, UFLA; E. A. LIMA, UFLA; U. R. FERNANDES, UFLA; R. E. ORLANDI, UFLA; S. A. D. RODRIGUES, UNB; F. M. CAIXETA, UNB; N. G. ALVES, UFLA; J. C. SOUZA, UFLA; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MARGOT ALVES NUNES DODE, Cenargen; J. N. S. SALES, UFLA. |
Título: |
Nuclear maturation kinetics and in vitro fertilization of immature bovine oocytes injected into pre-ovulatory follicles. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Theriogenology, v. 166, p. 90-96, 2021. |
DOI: |
https://doi.org/10.1016/j.theriogenology.2020.12.021 |
Idioma: |
Inglês |
Conteúdo: |
The maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intrafollicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P < 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of metaphase II (P ¼ 0.001) and greater rates of degenerated (P ¼ 0.001) and parthenogenetically activated (P ¼ 0.001) oocytes. In experiment 2, the rates of polyspermy and degenerated were similar between Groups. However, the rate of fertilized oocytes was greater (P ¼ 0.05) in oocytes in the MatFol20 Group. It is concluded that oocyte in vivo maturation for 19.8 h after IFOT does not compromise the nuclear maturation kinetics and increases in vitro fertilization rates. However, the extra 10 h of intra-follicular incubation time decreased oocyte viability. MenosThe maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intrafollicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P < 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of met... Mostrar Tudo |
Palavras-Chave: |
Fertilização in-vitro; IFOT; Intra-follicular. |
Thesagro: |
Bovino; Fertilidade Animal. |
Thesaurus Nal: |
Embryo (animal). |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03144naa a2200361 a 4500 001 2133325 005 2021-09-15 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.theriogenology.2020.12.021$2DOI 100 1 $aSIMÕES, L. M. S. 245 $aNuclear maturation kinetics and in vitro fertilization of immature bovine oocytes injected into pre-ovulatory follicles.$h[electronic resource] 260 $c2021 520 $aThe maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intrafollicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P < 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of metaphase II (P ¼ 0.001) and greater rates of degenerated (P ¼ 0.001) and parthenogenetically activated (P ¼ 0.001) oocytes. In experiment 2, the rates of polyspermy and degenerated were similar between Groups. However, the rate of fertilized oocytes was greater (P ¼ 0.05) in oocytes in the MatFol20 Group. It is concluded that oocyte in vivo maturation for 19.8 h after IFOT does not compromise the nuclear maturation kinetics and increases in vitro fertilization rates. However, the extra 10 h of intra-follicular incubation time decreased oocyte viability. 650 $aEmbryo (animal) 650 $aBovino 650 $aFertilidade Animal 653 $aFertilização in-vitro 653 $aIFOT 653 $aIntra-follicular 700 1 $aSANTOS, A. P. C. 700 1 $aBOTTINO, M. P. 700 1 $aLIMA, E. A. 700 1 $aFERNANDES, U. R. 700 1 $aORLANDI, R. E. 700 1 $aRODRIGUES, S. A. D. 700 1 $aCAIXETA, F. M. 700 1 $aALVES, N. G. 700 1 $aSOUZA, J. C. 700 1 $aQUINTAO, C. C. R. 700 1 $aCAMARGO, L. S. A. 700 1 $aDODE, M. A. N. 700 1 $aSALES, J. N. S. 773 $tTheriogenology$gv. 166, p. 90-96, 2021.
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Embrapa Gado de Leite (CNPGL) |
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Registros recuperados : 85 | |
9. | | CAMARGO, L. S. A.; VIANA, J. H. M.; RAMOS, A. A.; SÁ, W. F.; FERREIRA, A. M. Relationship between Oct-4 gene expression and bovine embryo produced by nuclear transfer. Acta Scientiae Veterinariae, v. 34, s550, 2006. Supl. 1. Edição dos resumos da 20ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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10. | | VIANA, J. H. M.; CAMARGO, L. S. A; FERREIRA, A. M.; SA, W. F.; MARQUES JUNIOR, A. P. Nascimento de bezerra gerada com auxílio das técnicas de punção folicular e fertilização in vitro no estado de Minas Gerais. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 53, n. 4, p. 477-478, 2001.Biblioteca(s): Embrapa Gado de Leite. |
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11. | | FONSECA, J. F.; BRUSCHI, J. H.; ZAMBRINI, F. N.; VIANA, J. H. M.; AMORIM, L. S.; CAMARGO, L. S. A. Superovulação de cabras utilizando a primeira onda folicular do ciclo estral. Acta Scientiae Veterinariae, Porto Alegre, v. 34, supl. 1, p. s509, 2006. Edição dos resumos da 20. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, MG, agosto 2006.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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12. | | FONSECA, J. F.; BRUSCHI, J. H.; ZAMBRINI, F. N.; VIANA, J. H. M.; AMORIM, L. S.; CAMARGO, L. S. A. Superovulation in does based on first follicular wave of the estrous cycle. Acta Scientiae Veterinariae, v. 34, s508, 2006. Supl. 1. Edição dos resumos da 20ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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13. | | FERREIRA, A. M.; SA, W. F.; VIANA, J. H. M.; FERNANDES, C. A. C.; CAMARGO, L. S. A. Taxa de nao retorno ao cio em 60 dias e taxa de prenhez em bovinos. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 49, n. 2, p. 261-267, 1997.Biblioteca(s): Embrapa Gado de Leite. |
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14. | | WOHLRES, S.; PINTO, I. S. B.; BATISTA, R. I. T. P.; FAZA, A. P.; PAIVA, F. P.; MAFFILI, V. V.; CAMARGO, L. S. A.; VIANA, J. H. M. Are superovulation protocols realy efficient for embryo production in inbred mice lineages? Acta Scientiae Veterinariae, v. 34, s584, 2006. Supl. 1. Edição dos resumos da 20 Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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15. | | BOITÉ, M. C.; CAMARGO, L. S. A.; GUIMARÃES, M. F. M.; SERAPIÃO, R. V.; WOHLRES-VIANA, S.; VIANA, J. H. M.; SÁ, W. F.; NOGUEIRA, L. A. G. Analysis of gene expression of in vitro produced bovine blastocyst cultured for 72 hours post-vitrification. In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 16., 2008, Budapest. Abstracts... Budapest, 2008. p. 186Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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16. | | DIAS, N. B.; CAMARGO, L. S. A.; SERAPIÃO, R. V.; FOLHADELLA, I. M.; RAMOS, A. A.; POLISSENI, J.; VIANA, J. H. M.; SÁ, W. F.; FERREIRA, A. M. Association of bovine sperm motility and viability with in vitro embryo production. Acta Scientiae Veterinariae, v. 34, s437, 2006. Supl. 1. Edição dos resumos da 20ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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17. | | CAMARGO, L. S. A.; VIANA, J. H. M.; SERAPIÃO, R. V.; GUIMARÃES, M. F. M.; SÁ, W. F. de; FERREIRA, A. de M. Assessment of HSP70-1 transcription levels in immature oocytes from Bos Taurus and Bos Indicus cows raised in a tropical climate. Reproduction, Fertility and Development, v. 19, n. 1, p. 247-248, 2007. Edição dos proceedings do Annual Conference of The Internatinal Embryo Transfer Society, 2007, Kyoto.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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18. | | FONSECA, J. F.; BRUSCHI, J. H.; VIANA, J. H. M.; CAMARGO, L. S. A.; SIQUEIRA, L. G. B.; SANTOS, M. L. Alternative days for prostaglandin administration in short-term protocols for estrous synchronization in goats. In: TSUKUBA MEETING FOR ANIMAL BIOTECHNOLOGY (TMAB), 2007, Tsukuba. Proceedings... Tsukuba, Japan: National Institute of Agrobiological Sciences - NIAS: National Institute of Livestock and Grassland Science (NILGS), 2007. p. 43-35.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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19. | | OLIVEIRA, R. S.; DIAS, A. J. B.; SÁ, W. F.; CAMARGO, L. S. A.; VIANA, J. H. M.; MORAES, G. A. Comparison of two vitrification protocols for in vitro produced bovine embryos. Acta Scientiae Veterinariae, v. 34, s476, 2006. Supl. 1. Edição dos resumos da 20ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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20. | | CAMARGO, L. S. A.; ARASHIRO, E. K. N.; VIANA, J. H. M.; PALHÃO, M. P.; FONSECA, J. F. da; SÁ, W. F.; FERREIRA, A. M. Characteristics of calves produced by oocyte pick-up and in vitro fertilization. Arquivos de Ciências Veterinárias e Zoologia da UNIPAR, Umuarama, v. 7, p. 152, 2004. Suplemento 1. Edição da IX Jornada de Medicina Veterinária da UNIPAR e VI Mostra Científica em Medicina Veterinária, 2004.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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Registros recuperados : 85 | |
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