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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
03/05/2010 |
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Data da última atualização: |
02/08/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
KITAJIMA, E. W.; CALEGARIO, R. F.; FREITAS-ASTUA, J.; ALBERTI, G. |
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Afiliação: |
Elliot Watanabe Kitajima, ESALQ; Renata F. Calegario, ESALQ; JULIANA DE FREITAS ASTUA, CNPMF; Gerd Alberti, Uni- Greitswald. |
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Título: |
Electron microscopic evidences that Citrus leprosis virus, cytoplasmic type (CiLV-C) only circulates in the vector Brevipalpus phoenicis (Acari: Tenuipalpidae) while other nuclear type Brevipalpus transmitted viruses do replicate. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In:MILBENKUNDLICHES KOLLOQUIUM, 7., 2009, Posen. Abstracts... Posen: Adam Mickiewicz Universität, 2009. p. 28-29. |
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Idioma: |
Inglês |
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Conteúdo: |
Electron microscopic (EM) examination of Brevipalpus phoenicis (Acari: Tenuipalpidae) colonizing Citrus leprosis virus, cytoplasmic type (CiLV-C)-infected sweet orange plants permitted the detection of bacilliform, virus-like particles only between membrane of adjacent cells in the vicinity of the prosomal gland complex and the midgut. The viral nature of these particles was assessed by immunolocalization using anti-CiLV-C capsid proteins. To explain the viral route from the midgut lumen to the duct of the prosomal gland complex two possibilities were considered: (a) intracellular, in which viral particles could be endo- and exocyted in the epithelium of the midgut and prosomal gland complex. (b) Paracellular, by which virions could pass through epithelial cell junctions in a process resembling diapedesis of leucocytes from the capilary vessels in vertebrates. Virions could be transported from midgut to the prosomal glands by a flux induced by the mite movement. Since no virions were ever found within cells. were favor the paracellar route. Also, because we could not detect the characteristic electron dense and vacuolated viroplasma in the mite tissues, we believe that CiLV-C does not multiply en the vector, just circulating in the mite body. Other biological and molecular evidences are in line with this thought. On the other hand, EM observations on several other Brevipalpus-transmitted viruses (BTV) of the nuclear type (nuclear viroplasm) as Coffee ringspot virus (CoRSV). Clerodendrum chlorotic spot virus (CICSV), citrus leprosis virus-nuclear type (CiLV-N) and Orchid fleck virus (OFV) revealed the presence of the rod-shaped virus-like particles in the nucleus and cytoplasm, and the lypical electron lucent nuclear viroplasm in the cells of the epithelium of the midgut and of the prosomal gland complex, as it occurs in infected leaf cells. The detection of the viroplasm strongly suggests that these viruses multiply in some tissues of the mite vector (B. phoenicis for CoRSV, CICSV, CiLV-N) and B. califomicus, for OFV). MenosElectron microscopic (EM) examination of Brevipalpus phoenicis (Acari: Tenuipalpidae) colonizing Citrus leprosis virus, cytoplasmic type (CiLV-C)-infected sweet orange plants permitted the detection of bacilliform, virus-like particles only between membrane of adjacent cells in the vicinity of the prosomal gland complex and the midgut. The viral nature of these particles was assessed by immunolocalization using anti-CiLV-C capsid proteins. To explain the viral route from the midgut lumen to the duct of the prosomal gland complex two possibilities were considered: (a) intracellular, in which viral particles could be endo- and exocyted in the epithelium of the midgut and prosomal gland complex. (b) Paracellular, by which virions could pass through epithelial cell junctions in a process resembling diapedesis of leucocytes from the capilary vessels in vertebrates. Virions could be transported from midgut to the prosomal glands by a flux induced by the mite movement. Since no virions were ever found within cells. were favor the paracellar route. Also, because we could not detect the characteristic electron dense and vacuolated viroplasma in the mite tissues, we believe that CiLV-C does not multiply en the vector, just circulating in the mite body. Other biological and molecular evidences are in line with this thought. On the other hand, EM observations on several other Brevipalpus-transmitted viruses (BTV) of the nuclear type (nuclear viroplasm) as Coffee ringspot virus (CoRSV). ... Mostrar Tudo |
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Thesagro: |
Brevipalpus Phoenicis; Vírus. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/748556/1/Electron-microscopic-evidences-that-Citrus-leprosis-virus-cytoplasmic-type.pdf
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Marc: |
LEADER 02788nam a2200169 a 4500
001 1748556
005 2023-08-02
008 2009 bl uuuu u00u1 u #d
100 1 $aKITAJIMA, E. W.
245 $aElectron microscopic evidences that Citrus leprosis virus, cytoplasmic type (CiLV-C) only circulates in the vector Brevipalpus phoenicis (Acari$bTenuipalpidae) while other nuclear type Brevipalpus transmitted viruses do replicate.$h[electronic resource]
260 $aIn:MILBENKUNDLICHES KOLLOQUIUM, 7., 2009, Posen. Abstracts... Posen: Adam Mickiewicz Universität, 2009. p. 28-29.$c2009
520 $aElectron microscopic (EM) examination of Brevipalpus phoenicis (Acari: Tenuipalpidae) colonizing Citrus leprosis virus, cytoplasmic type (CiLV-C)-infected sweet orange plants permitted the detection of bacilliform, virus-like particles only between membrane of adjacent cells in the vicinity of the prosomal gland complex and the midgut. The viral nature of these particles was assessed by immunolocalization using anti-CiLV-C capsid proteins. To explain the viral route from the midgut lumen to the duct of the prosomal gland complex two possibilities were considered: (a) intracellular, in which viral particles could be endo- and exocyted in the epithelium of the midgut and prosomal gland complex. (b) Paracellular, by which virions could pass through epithelial cell junctions in a process resembling diapedesis of leucocytes from the capilary vessels in vertebrates. Virions could be transported from midgut to the prosomal glands by a flux induced by the mite movement. Since no virions were ever found within cells. were favor the paracellar route. Also, because we could not detect the characteristic electron dense and vacuolated viroplasma in the mite tissues, we believe that CiLV-C does not multiply en the vector, just circulating in the mite body. Other biological and molecular evidences are in line with this thought. On the other hand, EM observations on several other Brevipalpus-transmitted viruses (BTV) of the nuclear type (nuclear viroplasm) as Coffee ringspot virus (CoRSV). Clerodendrum chlorotic spot virus (CICSV), citrus leprosis virus-nuclear type (CiLV-N) and Orchid fleck virus (OFV) revealed the presence of the rod-shaped virus-like particles in the nucleus and cytoplasm, and the lypical electron lucent nuclear viroplasm in the cells of the epithelium of the midgut and of the prosomal gland complex, as it occurs in infected leaf cells. The detection of the viroplasm strongly suggests that these viruses multiply in some tissues of the mite vector (B. phoenicis for CoRSV, CICSV, CiLV-N) and B. califomicus, for OFV).
650 $aBrevipalpus Phoenicis
650 $aVírus
700 1 $aCALEGARIO, R. F.
700 1 $aFREITAS-ASTUA, J.
700 1 $aALBERTI, G.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
09/03/2016 |
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Data da última atualização: |
09/03/2016 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
CUNHA, A. J. da; SANTOS, J. C. F.; OLIVEIRA, R. A. de; ROCHA, L. B.; MARTINS, N. S. |
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Afiliação: |
AQUILES JUNIOR DA CUNHA, UNICERP; JULIO CESAR FREITAS SANTOS, SAPC; REGINALDO ANDRÉ DE OLIVEIRA, Agrônomo; LUCAS BAISTA ROCHA, UNICERP; NATÁLIA SILVA MARTINS, UNICERP. |
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Título: |
Desenvolvimento inicial de cafeeiros provenientes de mudas formadas em diferentes recipientes. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 9., 2015, Curitiba. Anais... Brasília, DF: Embrapa Café, 2015. |
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Idioma: |
Português |
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Conteúdo: |
A tecnologia de produção de mudas tem evoluído muito rapidamente nos últimos anos, principalmente no que se refere ao tipo de recipiente. O objetivo desse trabalho foi avaliar o desenvolvimento vegetativo inicial de cafeeiros provenientes de mudas formadas em diferentes recipientes. Os recipientes testados foram as tradicionais sacolas plásticas nas dimensões 10 x 20 cm, 11 x 20 cm, 12 x 20 cm, 13 x 20 cm, tubete de polietileno (180 cm³) e sacolas plásticas (260 cm ³). Utilizou-se delineamento experimental em blocos casualizados, sendo seis tratamentos com quatro repetições. As mudas foram avaliadas ao longo de sete meses, determinando-se o replantio, a altura, o diâmetro, número de ramos plagiotrópicos e número de nós das mudas de cafeeiro. Para as cinco características analisadas, o melhor resultado observado no desenvolvimento inicial do cafeeiro foi com o uso da tradicional sacola plástica nas dimensões 13 x 20 cm. Os resultados mostraram que mesmo com a adoção de novas tecnologias na produção de mudas de cafeeiro, quando se trata de plantio em sequeiro, o recipiente mais indicado são as sacolas plásticas. As sacolas plásticas e tubete de polietileno se mostraram inferiores para a produção de mudas de café, quando estas se destinam ao cultivo de sequeiro. |
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Palavras-Chave: |
Sacola. |
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Thesagro: |
Café; Muda; Tubete. |
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Thesaurus NAL: |
Bags; Seedlings; Tubes. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140892/1/Desenvolvimento-inicial-de-cafeeiros.pdf
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Marc: |
LEADER 02027nam a2200241 a 4500
001 2040301
005 2016-03-09
008 2015 bl uuuu u00u1 u #d
100 1 $aCUNHA, A. J. da
245 $aDesenvolvimento inicial de cafeeiros provenientes de mudas formadas em diferentes recipientes.$h[electronic resource]
260 $aIn: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 9., 2015, Curitiba. Anais... Brasília, DF: Embrapa Café$c2015
520 $aA tecnologia de produção de mudas tem evoluído muito rapidamente nos últimos anos, principalmente no que se refere ao tipo de recipiente. O objetivo desse trabalho foi avaliar o desenvolvimento vegetativo inicial de cafeeiros provenientes de mudas formadas em diferentes recipientes. Os recipientes testados foram as tradicionais sacolas plásticas nas dimensões 10 x 20 cm, 11 x 20 cm, 12 x 20 cm, 13 x 20 cm, tubete de polietileno (180 cm³) e sacolas plásticas (260 cm ³). Utilizou-se delineamento experimental em blocos casualizados, sendo seis tratamentos com quatro repetições. As mudas foram avaliadas ao longo de sete meses, determinando-se o replantio, a altura, o diâmetro, número de ramos plagiotrópicos e número de nós das mudas de cafeeiro. Para as cinco características analisadas, o melhor resultado observado no desenvolvimento inicial do cafeeiro foi com o uso da tradicional sacola plástica nas dimensões 13 x 20 cm. Os resultados mostraram que mesmo com a adoção de novas tecnologias na produção de mudas de cafeeiro, quando se trata de plantio em sequeiro, o recipiente mais indicado são as sacolas plásticas. As sacolas plásticas e tubete de polietileno se mostraram inferiores para a produção de mudas de café, quando estas se destinam ao cultivo de sequeiro.
650 $aBags
650 $aSeedlings
650 $aTubes
650 $aCafé
650 $aMuda
650 $aTubete
653 $aSacola
700 1 $aSANTOS, J. C. F.
700 1 $aOLIVEIRA, R. A. de
700 1 $aROCHA, L. B.
700 1 $aMARTINS, N. S.
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Embrapa Café (CNPCa) |
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