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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
20/11/2020 |
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Data da última atualização: |
20/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
D'AVILA, L. S.; FILIPPI, M. C. C. de; CAFÉ-FILHO, A. C. |
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Afiliação: |
LEILANE SILVEIRA D'AVILA; MARTA CRISTINA CORSI DE FILIPPI, CNPAF; ADALBERTO C. CAFÉ-FILHO, UNB. |
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Título: |
Sensitivity of Pyricularia oryzae populations to fungicides over a 26-year time frame in Brazil. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Plant Disease, 2020. |
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ISSN: |
1943-7692 |
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DOI: |
https://doi.org/10.1094/PDIS-08-20-1806-RE |
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Idioma: |
Inglês |
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Notas: |
Published Online:2 Nov 2020. |
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Conteúdo: |
The long-term dynamics of fungicide resistance of the rice blast fungus Pyricularia oryzae was monitored by examining the reaction of the fungal field isolates, collected over a period of 26 years, to the active ingredients of commercially relevant fungicides. The in vitro sensitivity of all isolates was measured against Quinone outside inhibitors (QoI), Melanin biosynthesis inhibitors (MBI) and Sterol demethylation inhibitor (DMI) fungicides, namely azoxystrobin (QoI), tricyclazole (MBI), tebuconazole (DMI), and trifloxystrobin + tebuconazole (QoI + DMI). Over the 26-year collection period, a gradual rise in the EC50 estimates for mycelial growth sensitivity was observed for all fungicides, but most strikingly for azoxystrobin. A rise in conidial germination and appressorium formation was also noted, most markedly for azoxystrobin. Consistently, the earlier isolates were much more sensitive to the active ingredients than the more contemporary isolates. The sequencing of the amplified cyt b fragment distinguished two haplotypes, H1 and H2. Haplotype H1 (six isolates) contained the G to C transversion at codon 143 (resulting in change G143A), linked to the resistant phenotype QoI-R. Haplotype H2 (40 isolates), gathered the isolates sensitive to QoI. This work documents the gradual rise in the frequency of fungicide resistant isolates in Pyricularia oryzae rice populations on a long-term basis. |
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Thesagro: |
Arroz; Brusone; Doença de Planta; Fungicida; Oryza Sativa; Pyricularia Oryzae. |
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Thesaurus Nal: |
Blast disease; Fungicides; Pyricularia; Rice. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02262naa a2200301 a 4500
001 2126858
005 2020-11-20
008 2020 bl uuuu u00u1 u #d
022 $a1943-7692
024 7 $ahttps://doi.org/10.1094/PDIS-08-20-1806-RE$2DOI
100 1 $aD'AVILA, L. S.
245 $aSensitivity of Pyricularia oryzae populations to fungicides over a 26-year time frame in Brazil.$h[electronic resource]
260 $c2020
500 $aPublished Online:2 Nov 2020.
520 $aThe long-term dynamics of fungicide resistance of the rice blast fungus Pyricularia oryzae was monitored by examining the reaction of the fungal field isolates, collected over a period of 26 years, to the active ingredients of commercially relevant fungicides. The in vitro sensitivity of all isolates was measured against Quinone outside inhibitors (QoI), Melanin biosynthesis inhibitors (MBI) and Sterol demethylation inhibitor (DMI) fungicides, namely azoxystrobin (QoI), tricyclazole (MBI), tebuconazole (DMI), and trifloxystrobin + tebuconazole (QoI + DMI). Over the 26-year collection period, a gradual rise in the EC50 estimates for mycelial growth sensitivity was observed for all fungicides, but most strikingly for azoxystrobin. A rise in conidial germination and appressorium formation was also noted, most markedly for azoxystrobin. Consistently, the earlier isolates were much more sensitive to the active ingredients than the more contemporary isolates. The sequencing of the amplified cyt b fragment distinguished two haplotypes, H1 and H2. Haplotype H1 (six isolates) contained the G to C transversion at codon 143 (resulting in change G143A), linked to the resistant phenotype QoI-R. Haplotype H2 (40 isolates), gathered the isolates sensitive to QoI. This work documents the gradual rise in the frequency of fungicide resistant isolates in Pyricularia oryzae rice populations on a long-term basis.
650 $aBlast disease
650 $aFungicides
650 $aPyricularia
650 $aRice
650 $aArroz
650 $aBrusone
650 $aDoença de Planta
650 $aFungicida
650 $aOryza Sativa
650 $aPyricularia Oryzae
700 1 $aFILIPPI, M. C. C. de
700 1 $aCAFÉ-FILHO, A. C.
773 $tPlant Disease, 2020.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca |
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Classificação |
Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Pecuária Sudeste. |
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Data corrente: |
09/10/2020 |
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Data da última atualização: |
09/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SOUZA, M. M. de; ZERLOTINI NETO, A.; ROCHA, M. I. P.; BRUSCADIN, J. J.; DINIZ, W. J. da S.; CARDOSO, T. F.; CESAR, A. S. M.; AFONSO, J.; ANDRADE, B. G. N.; MUDADU, M. de A.; MOKRY, F. B.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; NICIURA, S. C. M.; COUTINHO, L. L.; REGITANO, L. C. de A. |
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Afiliação: |
MARCELA MARIA DE SOUZA, UFSCar; ADHEMAR ZERLOTINI NETO, CNPTIA; MARINA IBELLI PEREIRA ROCHA, UFSCar; JENNIFER JESSICA BRUSCADIN, UFSCar; WELLISON JARLES DA SILVA DINIZ, UFSCar; TAINÃ FIGUEIREDO CARDOSO; ALINE SILVA MELLO CESAR, ESALQ/USP; JULIANA AFONSO, UFSCar; BRUNO GABRIEL NASCIMENTO ANDRADE, UFSCar; MAURICIO DE ALVARENGA MUDADU, CNPTIA; FABIANA BARICHELLO MOKRY, UFSCar; POLYANA CRISTINE TIZIOTO, NGS Soluções Genômicas, Piracicaba; PRISCILA SILVA NEUBERN DE OLIVEIRA; SIMONE CRISTINA MEO NICIURA, CPPSE; LUIZ LEHMANN COUTINHO, ESALQ/USP; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
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Título: |
Allele-specific expression is widespread in Bos indicus muscle and affects meat quality candidate genes. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Scientific Reports, v. 10, n. 1, p. 1-11, 2020. |
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DOI: |
https://doi.org/10.1038/S41598-020-67089-0 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Adhemar Zerlotini. Article number: 10204. |
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Conteúdo: |
Differences between the expression of the two alleles of a gene are known as allele-specific expression (ASE), a common event in the transcriptome of mammals. Despite ASE being a source of phenotypic variation, its occurrence and effects on genetic prediction of economically relevant traits are still unexplored in bovines. Furthermore, as ASE events are likely driven by cis-regulatory mutations, scanning them throughout the bovine genome represents a significant step to elucidate the mechanisms underlying gene expression regulation. To address this question in a Bos indicus population, we built the ASE profile of the skeletal muscle tissue of 190 Nelore steers, using RNA sequencing data and SNPs genotypes from the Illumina BovineHD BeadChip (770 K bp). After quality control, 820 SNPs showed at least one sample with ASE. These SNPs were widespread among all autosomal chromosomes, being 32.01% found in 3'UTR and 31.41% in coding regions. We observed a considerable variation of ASE profile among individuals, which highlighted the need for biological replicates in ASE studies. Functional analysis revealed that ASE genes play critical biological functions in the development and maintenance of muscle tissue. Additionally, some of these genes were previously reported as associated with beef production and quality traits in livestock, thus indicating a possible source of bias on genomic predictions for these traits. |
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Palavras-Chave: |
Allele-specific expression; Allelic expression; Expressão gênica; Regulação da expressão gênica. |
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Thesagro: |
Bos Indicus. |
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Thesaurus NAL: |
Gene expression regulation. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216548/1/AP-Allele-specific-2020.pdf
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Marc: |
LEADER 02698naa a2200397 a 4500
001 2125395
005 2020-10-09
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/S41598-020-67089-0$2DOI
100 1 $aSOUZA, M. M. de
245 $aAllele-specific expression is widespread in Bos indicus muscle and affects meat quality candidate genes.$h[electronic resource]
260 $c2020
500 $aNa publicação: Adhemar Zerlotini. Article number: 10204.
520 $aDifferences between the expression of the two alleles of a gene are known as allele-specific expression (ASE), a common event in the transcriptome of mammals. Despite ASE being a source of phenotypic variation, its occurrence and effects on genetic prediction of economically relevant traits are still unexplored in bovines. Furthermore, as ASE events are likely driven by cis-regulatory mutations, scanning them throughout the bovine genome represents a significant step to elucidate the mechanisms underlying gene expression regulation. To address this question in a Bos indicus population, we built the ASE profile of the skeletal muscle tissue of 190 Nelore steers, using RNA sequencing data and SNPs genotypes from the Illumina BovineHD BeadChip (770 K bp). After quality control, 820 SNPs showed at least one sample with ASE. These SNPs were widespread among all autosomal chromosomes, being 32.01% found in 3'UTR and 31.41% in coding regions. We observed a considerable variation of ASE profile among individuals, which highlighted the need for biological replicates in ASE studies. Functional analysis revealed that ASE genes play critical biological functions in the development and maintenance of muscle tissue. Additionally, some of these genes were previously reported as associated with beef production and quality traits in livestock, thus indicating a possible source of bias on genomic predictions for these traits.
650 $aGene expression regulation
650 $aBos Indicus
653 $aAllele-specific expression
653 $aAllelic expression
653 $aExpressão gênica
653 $aRegulação da expressão gênica
700 1 $aZERLOTINI NETO, A.
700 1 $aROCHA, M. I. P.
700 1 $aBRUSCADIN, J. J.
700 1 $aDINIZ, W. J. da S.
700 1 $aCARDOSO, T. F.
700 1 $aCESAR, A. S. M.
700 1 $aAFONSO, J.
700 1 $aANDRADE, B. G. N.
700 1 $aMUDADU, M. de A.
700 1 $aMOKRY, F. B.
700 1 $aTIZIOTO, P. C.
700 1 $aOLIVEIRA, P. S. N. de
700 1 $aNICIURA, S. C. M.
700 1 $aCOUTINHO, L. L.
700 1 $aREGITANO, L. C. de A.
773 $tScientific Reports$gv. 10, n. 1, p. 1-11, 2020.
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Embrapa Agricultura Digital (CNPTIA) |
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