Data/hora: 29/05/2024 10:48:59

Biblioteca(s): 	Embrapa Arroz e Feijão.	
Data corrente: 	20/11/2020	
Data da última atualização: 	20/11/2020	
Autoria: 	D'AVILA, L. S.; FILIPPI, M. C. C. de; CAFÉ-FILHO, A. C.	
Afiliação: 	LEILANE SILVEIRA D'AVILA; MARTA CRISTINA CORSI DE FILIPPI, CNPAF; ADALBERTO C. CAFÉ-FILHO, UNB.	
Título: 	Sensitivity of Pyricularia oryzae populations to fungicides over a 26-year time frame in Brazil.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	Plant Disease, 2020.	
ISSN: 	1943-7692	
DOI: 	https://doi.org/10.1094/PDIS-08-20-1806-RE	
Idioma: 	Inglês	
Notas: 	Published Online:2 Nov 2020.	
Conteúdo: 	The long-term dynamics of fungicide resistance of the rice blast fungus Pyricularia oryzae was monitored by examining the reaction of the fungal field isolates, collected over a period of 26 years, to the active ingredients of commercially relevant fungicides. The in vitro sensitivity of all isolates was measured against Quinone outside inhibitors (QoI), Melanin biosynthesis inhibitors (MBI) and Sterol demethylation inhibitor (DMI) fungicides, namely azoxystrobin (QoI), tricyclazole (MBI), tebuconazole (DMI), and trifloxystrobin + tebuconazole (QoI + DMI). Over the 26-year collection period, a gradual rise in the EC50 estimates for mycelial growth sensitivity was observed for all fungicides, but most strikingly for azoxystrobin. A rise in conidial germination and appressorium formation was also noted, most markedly for azoxystrobin. Consistently, the earlier isolates were much more sensitive to the active ingredients than the more contemporary isolates. The sequencing of the amplified cyt b fragment distinguished two haplotypes, H1 and H2. Haplotype H1 (six isolates) contained the G to C transversion at codon 143 (resulting in change G143A), linked to the resistant phenotype QoI-R. Haplotype H2 (40 isolates), gathered the isolates sensitive to QoI. This work documents the gradual rise in the frequency of fungicide resistant isolates in Pyricularia oryzae rice populations on a long-term basis.	
Thesagro: 	Arroz; Brusone; Doença de Planta; Fungicida; Oryza Sativa; Pyricularia Oryzae.	
Thesaurus Nal: 	Blast disease; Fungicides; Pyricularia; Rice.	
Categoria do assunto: 	H Saúde e Patologia	
Registro original: 	Embrapa Arroz e Feijão (CNPAF)	
Exemplares: 	CNPAF - (35968-1) - UPC - AP - DD - 2020 -  - 2020 - DIS