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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais. |
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Data corrente: |
14/03/2014 |
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Data da última atualização: |
26/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALMEIDA, I. G.; IANELLA, P.; FARIA, M. T.; PAIVA, S. R.; CAETANO, A. R. |
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Afiliação: |
PATRICIA IANELLA, DPD. |
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Título: |
Bulked segregant analysis of the pirarucu (Arapaima gigas) genome for identification of sex-specific molecular markers. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Genetics and Molecular Research v. 12, n. 4, p. 6299-6308, Dec. 2013. |
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DOI: |
http://dx.doi.org/10.4238/2013.December.4.17 |
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Idioma: |
Inglês |
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Conteúdo: |
Arapaima gigas (Osteoglossidae) is one of the largest fish species in the Amazon Basin, attaining lengths of over 2.5 m and weights of over 100 kg. Its flesh is prized, and it has great potential for production in aquaculture systems. However, live pirarucu cannot be reliably sexed visually, even after sexual development, since this species does not have clear external sexual dimorphism. Simple and inexpensive methods for sexing immature pirarucu based on DNA markers would facilitate production of this species in commercial operations. We analyzed A. gigas male and female DNA pools with 566 RAPD primers, generating 2609 fragments, with an estimated 1341 segregating polymorphic markers, and an estimated average spacing of 714 kb, which corresponds to less than 0.1% of the species' genome. Two putative sex-specific fragments were initially identified in bulked samples; but they were not confirmed in a study of individual male and female samples. We suggest that A. gigas has developed a non-chromosomal system of sex determination or, alternatively, that the species has undergone a recent loss of the chromosome carrying the sex-determining locus. |
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Palavras-Chave: |
Marcadores moleculares; Peixe neotropical; RAPD. |
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Thesagro: |
Genoma; Marcador molecular; Peixe de água doce; Pirarucu. |
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Thesaurus Nal: |
Arapaima gigas; Freshwater fish; Genetic markers; Genome. |
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Categoria do assunto: |
--
G Melhoramento Genético
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/171706/1/gmr3157.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/99211/1/gmr3157.pdf
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Marc: |
LEADER 02111naa a2200313 a 4500
001 2007932
005 2018-01-26
008 2013 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/2013.December.4.17$2DOI
100 1 $aALMEIDA, I. G.
245 $aBulked segregant analysis of the pirarucu (Arapaima gigas) genome for identification of sex-specific molecular markers.$h[electronic resource]
260 $c2013
520 $aArapaima gigas (Osteoglossidae) is one of the largest fish species in the Amazon Basin, attaining lengths of over 2.5 m and weights of over 100 kg. Its flesh is prized, and it has great potential for production in aquaculture systems. However, live pirarucu cannot be reliably sexed visually, even after sexual development, since this species does not have clear external sexual dimorphism. Simple and inexpensive methods for sexing immature pirarucu based on DNA markers would facilitate production of this species in commercial operations. We analyzed A. gigas male and female DNA pools with 566 RAPD primers, generating 2609 fragments, with an estimated 1341 segregating polymorphic markers, and an estimated average spacing of 714 kb, which corresponds to less than 0.1% of the species' genome. Two putative sex-specific fragments were initially identified in bulked samples; but they were not confirmed in a study of individual male and female samples. We suggest that A. gigas has developed a non-chromosomal system of sex determination or, alternatively, that the species has undergone a recent loss of the chromosome carrying the sex-determining locus.
650 $aArapaima gigas
650 $aFreshwater fish
650 $aGenetic markers
650 $aGenome
650 $aGenoma
650 $aMarcador molecular
650 $aPeixe de água doce
650 $aPirarucu
653 $aMarcadores moleculares
653 $aPeixe neotropical
653 $aRAPD
700 1 $aIANELLA, P.
700 1 $aFARIA, M. T.
700 1 $aPAIVA, S. R.
700 1 $aCAETANO, A. R.
773 $tGenetics and Molecular Research$gv. 12, n. 4, p. 6299-6308, Dec. 2013.
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Embrapa Unidades Centrais (AI-SEDE) |
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| 1. |  | SCHUINGES, C. de O.; LIMA, M. G. de; SARDINHA, G. H. R.; PIMENTA, A. L.; MONTEIRO, C. C.; AMBRÓSIO, C. E.; MARTINS, D. dos S.; COSTA, G. de M. Morfologia da traqueia e lobação pulmonar de Leopardus pardalis (jaguatirica). Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 8, p. 897-903, ago. 2017. Título em inglês: Morphology of the trachea and lung lobation of Leopardus pardalis (ocelot).| Biblioteca(s): Embrapa Unidades Centrais. |
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