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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
13/04/2021 |
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Data da última atualização: |
13/04/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUSA, T. P. de; CHAIBUB, A. A.; CORTES, M. V. de C. B.; BATISTA, T. F. C.; BEZERRA, G. de A.; SILVA, G. B. da; FILIPPI, M. C. C. de. |
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Afiliação: |
THATYANE PEREIRA DE SOUSA, UFG; AMANDA ABDALLAH CHAIBUB, UFG; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; TELMA FATIMA COELHO BATISTA, UNIVERSIDADE FEDERAL RURAL DA AMAZONIA; GUSTAVO DE ANDRADE BEZERRA, UFG; GISELE BARATA DA SILVA, UNIVERSIDADE FEDERAL RURAL DA AMAZONIA; MARTA CRISTINA CORSI DE FILIPPI, CNPAF. |
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Título: |
Molecular identification of Trichoderma sp. isolates and biochemical characterization of antagonistic interaction against rice blast. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Archives of Microbiology, 2021. |
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ISSN: |
0302-8933 |
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DOI: |
https://doi.org/10.1007/s00203-021-02307-5 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to identify four isolates of Trichoderma sp. (Ufra.T06, Ufra.T09, Ufra.T12, and Ufra.T52) and characterize their interaction with Magnaporthe oryzae in vitro and in vivo conditions. The four isolates of Trichoderma sp. were sequenced, investigated as an antagonist against M. oryzae in five Petri plate assays, and as an inhibitor of conidial germination appressoria formation. Finally, were quantified the lytic activity of chitinase (CHI), glucanase (GLU), and protease (PRO) during co-cultivation of Trichoderma sp. and M. oryzae. In vivo, leaf blast suppression was evaluated intwo assays: simultaneous and curative application. Both in vitro and in vivo assays were scanned by electron microscopy (SEM). All isolates were identified as Trichoderma asperellum. All in vitro Petri plates assays reduced M. oryzae colony growth (paired?91.18% by Ufra.T09, volatile metabolites?all isolates equally reduced, non-volatile?68.33% by Ufra.T06,thermostability?99.77% by Ufra.T52 and co-cultivate?64.25% by Ufra.T52). The filtrates and conidia suspensions for T. asperellum isolates inhibited the conidia germination and appressoria formation significantly. In co-cultivate (mycelial or cell wall), all enzymes (GLU, CHI, and PRO) and times (24, 48, and 72 h) showed increased activity. In vivo, reduced leafblast severity until 94.64% (Ufra.T52cs) in a simultaneous and until 85% (Ufra.T09 24 and 48 hasi) in a curative application. T. asperellum isolates showed efficient control of M. oryzae by mycoparasitism, and antibiosis mechanisms were interfered with by the M. oryzae infection process. MenosThis study aimed to identify four isolates of Trichoderma sp. (Ufra.T06, Ufra.T09, Ufra.T12, and Ufra.T52) and characterize their interaction with Magnaporthe oryzae in vitro and in vivo conditions. The four isolates of Trichoderma sp. were sequenced, investigated as an antagonist against M. oryzae in five Petri plate assays, and as an inhibitor of conidial germination appressoria formation. Finally, were quantified the lytic activity of chitinase (CHI), glucanase (GLU), and protease (PRO) during co-cultivation of Trichoderma sp. and M. oryzae. In vivo, leaf blast suppression was evaluated intwo assays: simultaneous and curative application. Both in vitro and in vivo assays were scanned by electron microscopy (SEM). All isolates were identified as Trichoderma asperellum. All in vitro Petri plates assays reduced M. oryzae colony growth (paired?91.18% by Ufra.T09, volatile metabolites?all isolates equally reduced, non-volatile?68.33% by Ufra.T06,thermostability?99.77% by Ufra.T52 and co-cultivate?64.25% by Ufra.T52). The filtrates and conidia suspensions for T. asperellum isolates inhibited the conidia germination and appressoria formation significantly. In co-cultivate (mycelial or cell wall), all enzymes (GLU, CHI, and PRO) and times (24, 48, and 72 h) showed increased activity. In vivo, reduced leafblast severity until 94.64% (Ufra.T52cs) in a simultaneous and until 85% (Ufra.T09 24 and 48 hasi) in a curative application. T. asperellum isolates showed efficient control of M... Mostrar Tudo |
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Palavras-Chave: |
Antagonism; Bioagent; Mycoparasitism. |
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Thesagro: |
Antagonismo; Arroz; Brusone; Controle Biológico; Marcador Molecular; Oryza Sativa; Trichoderma. |
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Thesaurus Nal: |
Biological control; Mycoparasites; Rice. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02690naa a2200373 a 4500
001 2131247
005 2021-04-13
008 2021 bl uuuu u00u1 u #d
022 $a0302-8933
024 7 $ahttps://doi.org/10.1007/s00203-021-02307-5$2DOI
100 1 $aSOUSA, T. P. de
245 $aMolecular identification of Trichoderma sp. isolates and biochemical characterization of antagonistic interaction against rice blast.$h[electronic resource]
260 $c2021
520 $aThis study aimed to identify four isolates of Trichoderma sp. (Ufra.T06, Ufra.T09, Ufra.T12, and Ufra.T52) and characterize their interaction with Magnaporthe oryzae in vitro and in vivo conditions. The four isolates of Trichoderma sp. were sequenced, investigated as an antagonist against M. oryzae in five Petri plate assays, and as an inhibitor of conidial germination appressoria formation. Finally, were quantified the lytic activity of chitinase (CHI), glucanase (GLU), and protease (PRO) during co-cultivation of Trichoderma sp. and M. oryzae. In vivo, leaf blast suppression was evaluated intwo assays: simultaneous and curative application. Both in vitro and in vivo assays were scanned by electron microscopy (SEM). All isolates were identified as Trichoderma asperellum. All in vitro Petri plates assays reduced M. oryzae colony growth (paired?91.18% by Ufra.T09, volatile metabolites?all isolates equally reduced, non-volatile?68.33% by Ufra.T06,thermostability?99.77% by Ufra.T52 and co-cultivate?64.25% by Ufra.T52). The filtrates and conidia suspensions for T. asperellum isolates inhibited the conidia germination and appressoria formation significantly. In co-cultivate (mycelial or cell wall), all enzymes (GLU, CHI, and PRO) and times (24, 48, and 72 h) showed increased activity. In vivo, reduced leafblast severity until 94.64% (Ufra.T52cs) in a simultaneous and until 85% (Ufra.T09 24 and 48 hasi) in a curative application. T. asperellum isolates showed efficient control of M. oryzae by mycoparasitism, and antibiosis mechanisms were interfered with by the M. oryzae infection process.
650 $aBiological control
650 $aMycoparasites
650 $aRice
650 $aAntagonismo
650 $aArroz
650 $aBrusone
650 $aControle Biológico
650 $aMarcador Molecular
650 $aOryza Sativa
650 $aTrichoderma
653 $aAntagonism
653 $aBioagent
653 $aMycoparasitism
700 1 $aCHAIBUB, A. A.
700 1 $aCORTES, M. V. de C. B.
700 1 $aBATISTA, T. F. C.
700 1 $aBEZERRA, G. de A.
700 1 $aSILVA, G. B. da
700 1 $aFILIPPI, M. C. C. de
773 $tArchives of Microbiology, 2021.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 86 | |
| 10. |  | TEIXEIRA, N. C.; DUARTE, L. T.; CORTES, M. V. de C. B.; FILIPPI, M. C. C. de; WENDLAND, A. Desenvolvimento de iniciadores para detecção e diferenciação de Magnaporthe oryzae em sementes de arroz. In: SEMINÁRIO JOVENS TALENTOS, 9., 2015, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2015. p. 115. (Embrapa Arroz e Feijão. Documentos, 309).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 11. | | SANTOS, T. D. dos; FERREIRA, E. P. de B.; KNUPP, A. M.; CORTES, M. V. de C. B. Caracterização de bactérias diazotróficas obtidas em diferentes regiões produtoras de feijoeiro-comum. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., 2016, Goiânia. Rumo aos novos desafios: [anais]. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2016. FertBio 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 12. | | ARRIEL, M. T.; CORTES, M. V. de C. B.; SILVA-LOBO, V. L.; FILIPPI, M. C. C. de. Determinação da taxa de crescimento e relação C:N para o cultivo de Burkholderia pyrrocinia, bioagente com potencial para suprimir a brusone no arroz. In: SEMINÁRIO JOVENS TALENTOS, 10., 2016, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2016. p. 88. (Embrapa Arroz e Feijão. Documentos, 311).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 19. | | SPERANDIO, E. M.; FILIPPI, M. C. C. de; CORTES, M. V. de C. B.; VALE, H. M. M. do. Quantificação de B-1,3-glucanase e ácido salicílico em plantas de arroz induzidas com isolado avirulento de Magnaporthe oryzae. In: SEMINÁRIO JOVENS TALENTOS, 10., 2016, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2016. p. 91. (Embrapa Arroz e Feijão. Documentos, 311).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 86 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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