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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
11/12/2000 |
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Data da última atualização: |
09/01/2024 |
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Autoria: |
GOH, S.; BYRNE, S. K.; ZHANG, J. L.; CHOW, A. W. |
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Título: |
Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Journal of Clinical Microbiology, v. 30, n. 7, p. 1642-1645, Jul. 1992. |
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DOI: |
10.1128/jcm.30.7.1642-1645.1992 |
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Idioma: |
Inglês |
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Conteúdo: |
Staphylocoagulase, a major phenotypic determinant of Staphylococcus aureus, exists in multiple allelic forms, in part because of the existence of gene variants within the 3'-end coding region. This region contains a series of repeating 81-bp DNA sequences which differ both in the number of tandem repeats and the location of AluI restriction sites among different isolates. Utilizing this finding, we developed a novel typing method for S. aureus based on polymerase chain reaction amplification of the variable region of the coagulase gene followed by AluI restriction enzyme digestion and analysis of restriction fragment length polymorphism (RFLP). Among 30 S. aureus isolates studied initially, a total of 10 distinct RFLP patterns were observed. There was excellent correlation of the RFLP patterns with typing of these isolates by multilocus enzyme electrophoresis at 20 chromosomal loci. This coagulase RFLP method was used to analyze an additional 39 S. aureus isolates and successfully traced the source of an outbreak of methicillin-resistant S. aureus infections at a local hospital. |
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Thesagro: |
Biologia Molecular; Microbiologia; Staphylococcus Aureus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01736naa a2200205 a 4500
001 1524191
005 2024-01-09
008 1992 bl uuuu u00u1 u #d
024 7 $a10.1128/jcm.30.7.1642-1645.1992$2DOI
100 1 $aGOH, S.
245 $aMolecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms.$h[electronic resource]
260 $c1992
520 $aStaphylocoagulase, a major phenotypic determinant of Staphylococcus aureus, exists in multiple allelic forms, in part because of the existence of gene variants within the 3'-end coding region. This region contains a series of repeating 81-bp DNA sequences which differ both in the number of tandem repeats and the location of AluI restriction sites among different isolates. Utilizing this finding, we developed a novel typing method for S. aureus based on polymerase chain reaction amplification of the variable region of the coagulase gene followed by AluI restriction enzyme digestion and analysis of restriction fragment length polymorphism (RFLP). Among 30 S. aureus isolates studied initially, a total of 10 distinct RFLP patterns were observed. There was excellent correlation of the RFLP patterns with typing of these isolates by multilocus enzyme electrophoresis at 20 chromosomal loci. This coagulase RFLP method was used to analyze an additional 39 S. aureus isolates and successfully traced the source of an outbreak of methicillin-resistant S. aureus infections at a local hospital.
650 $aBiologia Molecular
650 $aMicrobiologia
650 $aStaphylococcus Aureus
700 1 $aBYRNE, S. K.
700 1 $aZHANG, J. L.
700 1 $aCHOW, A. W.
773 $tJournal of Clinical Microbiology$gv. 30, n. 7, p. 1642-1645, Jul. 1992.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
03/01/2020 |
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Data da última atualização: |
03/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
FERNANDES, T. S. M.; ALCÂNTARA, D. B.; BARBOSA, P. G. A.; PAZ, M. S. DE O.; ZOCOLO, G. J.; NASCIMENTO, R. F. DO. |
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Afiliação: |
TATIANA SAINARA MAIA FERNANDES, Department of Analytical Chemistry and Physical Chemistry, Federal University of Ceara; DANIEL BARBOSA ALCÂNTARA, Department of Analytical Chemistry and Physical Chemistry, Federal University of Ceara; PABLO GORDIANO ALEXANDRE BARBOSA, Federal Institute of Education Science and Technology of Ceará, Sobral; MÁRIO SÉRGIO DE OLIVEIRA PAZ, Federal Institute of Education Science and Technology of Baiano, Guanambi; GUILHERME JULIAO ZOCOLO, CNPAT; RONALDO FERREIRA DO NASCIMENTO, Department of Analytical Chemistry and Physical Chemistry, Federal University of Ceara. |
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Título: |
Matrix effect evaluation and method validation of organophosphorus pesticide residues in bell peppers (Capsicum annuum L.) by GC-MS determination. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
International Journal of Environmental Analytical Chemistry, Abingdon, 16 p., 26 Aug. 2019. |
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DOI: |
https://doi.org/10.1080/03067319.2019.1657854 |
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Idioma: |
Inglês |
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Palavras-Chave: |
Efeito matricial; GCMS; QuEChERS; Resíduos de organofósforo. |
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Thesagro: |
Pimentão. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00857naa a2200241 a 4500
001 2118087
005 2020-01-03
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1080/03067319.2019.1657854$2DOI
100 1 $aFERNANDES, T. S. M.
245 $aMatrix effect evaluation and method validation of organophosphorus pesticide residues in bell peppers (Capsicum annuum L.) by GC-MS determination.$h[electronic resource]
260 $c2019
650 $aPimentão
653 $aEfeito matricial
653 $aGCMS
653 $aQuEChERS
653 $aResíduos de organofósforo
700 1 $aALCÂNTARA, D. B.
700 1 $aBARBOSA, P. G. A.
700 1 $aPAZ, M. S. DE O.
700 1 $aZOCOLO, G. J.
700 1 $aNASCIMENTO, R. F. DO
773 $tInternational Journal of Environmental Analytical Chemistry, Abingdon, 16 p., 26 Aug. 2019.
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Embrapa Agroindústria Tropical (CNPAT) |
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