Data/hora: 11/06/2024 10:57:55

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 26/10/2000
Data da última atualização: | 26/10/2000
Autoria: | HARDY, W. F.; HOLSTEN, R. D.; JACKSON, E. K.; BURNS, R. C.
Afiliação: | Central Research Department, Experimental Station, E. I. du Pont de Nemours and Company, Wilmington, Delaware.
Título: | The acetylene - ethylene assay for N2 fixation: laboratory and field evaluation.
Ano de publicação: | 1968
Fonte/Imprenta: | Plant Physiology
Volume: | 43
Páginas: | 1185-1207
Idioma: | Inglês
Conteúdo: | The methodology, characteristics and application of the sensitive C2H2-C2H4 assay for N2 fixation by nitrogenase preparations and bacterial cultural cultures in the laboratory and by legumes and free-living bacteria in situ is presented in this comprehensive report. This assay is based on the N2ase-catalyzed reduction of C2H2 to C2H2, gas chromatographic isolation of C2H2 and C2H4 quantitative measurement with a H2 -flame analyzer. As little as 1 uumole C2H4 can be detected, providing a sensitivity 10elevado a 3 -fold greater than is possible with 15N analysis.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (18731-1) - ADC - AP - -- -  -  -  - DIS