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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
03/07/2019 |
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Data da última atualização: |
01/10/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
QUADROS, C. da C. de; LIMA, K. O.; BUENO, C. H. L.; FOGACA, F. H. dos S.; ROCHA, M. da; PRENTICE, C. |
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Afiliação: |
Camila Da Costa De Quadros, UFRS; Karina Oliveira Lima, UFRS; Caio Hendrix Luz Bueno, UFRS; FABIOLA HELENA DOS SANTOS FOGACA, CTAA; Meritaine da Rocha, UFRS; Carlos Prentice, UFRS. |
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Título: |
Evaluation of the Antioxidant and Antimicrobial Activity of Protein Hydrolysates and Peptide Fractions Derived from Colossoma macropomum and Their Effect on Ground Beef Lipid Oxidation. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Aquatic Food Product Technology, 2019. p. 1-12. |
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ISSN: |
1547-0636 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to obtain protein hydrolysate from the mechanically separated meat of blackfin pacu to evaluate the influence by ultrafiltration in the antioxidant and antimicrobial activities of the peptidefractions obtained and to apply in ground beef to evaluate the lipid stability. The enzymatic hydrolysis was performed using the enzyme Protamex (pH 7.0, 60°C) for 240 min. The protein hydrolysate was fractionated by ultrafiltration. Then, the antioxidant capacity of the protein hydrolysate and the peptide fractions were evaluated in vitro by the methods of 2,2?-azinobis (3-ethylbenzothiazoline sulfonic acid) radical capture, 2,2-diphenyl-1-picrylhydrazyl radical-scavenging assay, reducing power, and hydroxyl radical scavenging activity. The antimicrobial activity of the samples was evaluated by disc-diffusion against Staphylococcus aureus and Escherichia coli. After evaluation, the peptide fractions did not present higher bioactivities than that shown for the hydrolysate. The protein hydrolysate was applied to ground beef, where the substances reactive to thiobarbituric acid and color were evaluated during 7 days of storage at 4°C. Lipid oxidation was reduced up to 60.9% and there was no modification of the natural coloration. Thus, the protein hydrolysate can be used as an alternative source of antioxidant for the preservation of refrigerated meats. |
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Palavras-Chave: |
Bioactivity; Mechanically separated meat of fish; Protamex. |
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Thesaurus Nal: |
Enzymatic hydrolysis. |
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Categoria do assunto: |
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Marc: |
LEADER 02214naa a2200241 a 4500
001 2110337
005 2019-10-01
008 2019 bl uuuu u00u1 u #d
022 $a1547-0636
100 1 $aQUADROS, C. da C. de
245 $aEvaluation of the Antioxidant and Antimicrobial Activity of Protein Hydrolysates and Peptide Fractions Derived from Colossoma macropomum and Their Effect on Ground Beef Lipid Oxidation.$h[electronic resource]
260 $c2019
520 $aThe objective of this study was to obtain protein hydrolysate from the mechanically separated meat of blackfin pacu to evaluate the influence by ultrafiltration in the antioxidant and antimicrobial activities of the peptidefractions obtained and to apply in ground beef to evaluate the lipid stability. The enzymatic hydrolysis was performed using the enzyme Protamex (pH 7.0, 60°C) for 240 min. The protein hydrolysate was fractionated by ultrafiltration. Then, the antioxidant capacity of the protein hydrolysate and the peptide fractions were evaluated in vitro by the methods of 2,2?-azinobis (3-ethylbenzothiazoline sulfonic acid) radical capture, 2,2-diphenyl-1-picrylhydrazyl radical-scavenging assay, reducing power, and hydroxyl radical scavenging activity. The antimicrobial activity of the samples was evaluated by disc-diffusion against Staphylococcus aureus and Escherichia coli. After evaluation, the peptide fractions did not present higher bioactivities than that shown for the hydrolysate. The protein hydrolysate was applied to ground beef, where the substances reactive to thiobarbituric acid and color were evaluated during 7 days of storage at 4°C. Lipid oxidation was reduced up to 60.9% and there was no modification of the natural coloration. Thus, the protein hydrolysate can be used as an alternative source of antioxidant for the preservation of refrigerated meats.
650 $aEnzymatic hydrolysis
653 $aBioactivity
653 $aMechanically separated meat of fish
653 $aProtamex
700 1 $aLIMA, K. O.
700 1 $aBUENO, C. H. L.
700 1 $aFOGACA, F. H. dos S.
700 1 $aROCHA, M. da
700 1 $aPRENTICE, C.
773 $tJournal of Aquatic Food Product Technology, 2019. p. 1-12.
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Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
19/10/2018 |
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Data da última atualização: |
19/10/2018 |
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Autoria: |
NEVES, R. C. S. M.; MAKINO, H.; CRUZ, T. P. P. S.; SILVEIRA, M. M.; SOUSA, V. R. F.; DUTRA, V.; LIMA, M. E. K. M.; BELLI, C. B. |
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Afiliação: |
Rita C. S. M. Neves, Hospital Veterinário - HOVET/Universidade Federal de Mato Grosso - UFMT; Hérica Makino, Programa de Pós-Graduação em Ciências Veterinárias - PPGVET/Universidade Federal do Mato Grosso - UFMT; Thalita P. P. S. Cruz, Programa de Pós-Graduação em Ciências Veterinárias - PPGVET/Universidade Federal do Mato Grosso - UFMT; Marcelo M. Silveira, Programa de Pós-Graduação em Ciências Veterinárias - PPGVET/Universidade Federal do Mato Grosso - UFMT; Valéria R. F. Sousa, Faculdade de Agronomia e Medicina Veterinária/Universidade Federal do Mato Grosso - UFMT; Valéria Dutra, Faculdade de Agronomia e Medicina Veterinária/Universidade Federal do Mato Grosso - UFMT; Marcia E. K. M. Lima, Médica Veterinária autônoma; Carla B. Belli, Faculdade de Medicina Veterinária e Zootecnia - FMVZ/Universidade de São Paulo - USP. |
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Título: |
In vitro and in vivo efficacy of tea tree essential oil for bacterial and yeast ear infections in dogs. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 38, n 8, p. 1597-1607, agosto 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em inglês: In vitro and in vivo efficacy of tea tree essential oil for bacterial and yeast ear infections in dogs. |
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Conteúdo: |
Otitis externa is a common complaint in dogs. Bacteria and yeasts are commonly involved and may perpetuate inflammatory reactions inside the ear canal. Otoscopy, cytological examination of secretion and microbiological culture embody forms of diagnosis. Cytology also has great use in accessing treatment evolution. Therapy usually consists of cleaning ear canals and subsequent use of antibiotics or antifungal products. As some of them may cause hypersensitivity and even ototoxicity, searching for new pharmacological bases is currently necessary and justifies this study, which aimed to evaluate in vitro and in vivo efficacy of tea tree essential oil for bacterial and yeast ear infections in dogs. Twenty-eight dogs from a particular shelter in Cuiabá (Mato Grosso, Brazil), presenting clinical signs of otitis externa, were enrolled in this clinical trial. In all of them, clinical and cytological evaluations, as well as culture and susceptibility testing of the affected ears were carried out. From each dog, one ear was treated with 5% tea tree essential oil lotion and the other with standard otic formulation, according to the type of infection (bacterial, yeast or both). In vitro susceptibility testings of all ear cultures, to the same drugs used in treatment, were also carried out. Culture results showed 62.5% bacterial and fungal infection, 33.9% bacterial infection and 3.6% fungal infection, from the 56 ear samples collected. The most common microorganisms isolated were Staphylococcus intermedius, Staphylococcus aureus, Proteus mirabilis and Malassezia pachydermatis. Gram‑positive bacteria were susceptible to gentamycin in 60.5% and resistant in 16.3% of the samples. Five percent tea tree essential oil formulation produced a 5mm clear zone of inhibition around the disks in one of the 63 samples evaluated. Pure (100%) tea tree essential oil formulation produced a 10mm clear zone of inhibition around the disks in four of the 63 samples evaluated, a 9mm zone in three samples, an 8mm zone in 16 samples, a 7mm zone in seven samples, a 6mm zone in two samples and there was no clear zone in 31 samples. Inhibition zones were produced by strains of Staphylococcus intermedius, Staphylococcus hyicus, Corynebacterium sp., Proteus mirabilis and Enterobacter sp. tea tree essential oil ear solution significantly induced remission of clinical signs both in bacterial and yeast ear infections. It also reduced as much Malassezia pachydermatis ear infection as the nystatin solution used in this study, while gentamycin solution showed better antibacterial effect. More studies should be conducted to evaluate in vitro diffusion properties of tea tree essential oil. Good antimicrobial spectrum and the absence of adverse reactions confirm the importance of developing a tea tree formulation as an alternative therapy for ear infections in dogs. MenosOtitis externa is a common complaint in dogs. Bacteria and yeasts are commonly involved and may perpetuate inflammatory reactions inside the ear canal. Otoscopy, cytological examination of secretion and microbiological culture embody forms of diagnosis. Cytology also has great use in accessing treatment evolution. Therapy usually consists of cleaning ear canals and subsequent use of antibiotics or antifungal products. As some of them may cause hypersensitivity and even ototoxicity, searching for new pharmacological bases is currently necessary and justifies this study, which aimed to evaluate in vitro and in vivo efficacy of tea tree essential oil for bacterial and yeast ear infections in dogs. Twenty-eight dogs from a particular shelter in Cuiabá (Mato Grosso, Brazil), presenting clinical signs of otitis externa, were enrolled in this clinical trial. In all of them, clinical and cytological evaluations, as well as culture and susceptibility testing of the affected ears were carried out. From each dog, one ear was treated with 5% tea tree essential oil lotion and the other with standard otic formulation, according to the type of infection (bacterial, yeast or both). In vitro susceptibility testings of all ear cultures, to the same drugs used in treatment, were also carried out. Culture results showed 62.5% bacterial and fungal infection, 33.9% bacterial infection and 3.6% fungal infection, from the 56 ear samples collected. The most common microorganisms isolated were Staphy... Mostrar Tudo |
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Palavras-Chave: |
Clínica; Clinics; Doença de ouvido; Levedura; Óleo essencial de melaleuca. |
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Thesagro: |
Bactéria; Cão; Fitoterapia. |
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Thesaurus NAL: |
Dogs; Ear diseases; Phytotherapy; Yeasts. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/184736/1/In-vitro-and-in-vivo-efficacy-of-tea-tree.pdf
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Marc: |
LEADER 03999naa a2200361 a 4500
001 2097776
005 2018-10-19
008 2018 bl uuuu u00u1 u #d
100 1 $aNEVES, R. C. S. M.
245 $aIn vitro and in vivo efficacy of tea tree essential oil for bacterial and yeast ear infections in dogs.$h[electronic resource]
260 $c2018
500 $aTítulo em inglês: In vitro and in vivo efficacy of tea tree essential oil for bacterial and yeast ear infections in dogs.
520 $aOtitis externa is a common complaint in dogs. Bacteria and yeasts are commonly involved and may perpetuate inflammatory reactions inside the ear canal. Otoscopy, cytological examination of secretion and microbiological culture embody forms of diagnosis. Cytology also has great use in accessing treatment evolution. Therapy usually consists of cleaning ear canals and subsequent use of antibiotics or antifungal products. As some of them may cause hypersensitivity and even ototoxicity, searching for new pharmacological bases is currently necessary and justifies this study, which aimed to evaluate in vitro and in vivo efficacy of tea tree essential oil for bacterial and yeast ear infections in dogs. Twenty-eight dogs from a particular shelter in Cuiabá (Mato Grosso, Brazil), presenting clinical signs of otitis externa, were enrolled in this clinical trial. In all of them, clinical and cytological evaluations, as well as culture and susceptibility testing of the affected ears were carried out. From each dog, one ear was treated with 5% tea tree essential oil lotion and the other with standard otic formulation, according to the type of infection (bacterial, yeast or both). In vitro susceptibility testings of all ear cultures, to the same drugs used in treatment, were also carried out. Culture results showed 62.5% bacterial and fungal infection, 33.9% bacterial infection and 3.6% fungal infection, from the 56 ear samples collected. The most common microorganisms isolated were Staphylococcus intermedius, Staphylococcus aureus, Proteus mirabilis and Malassezia pachydermatis. Gram‑positive bacteria were susceptible to gentamycin in 60.5% and resistant in 16.3% of the samples. Five percent tea tree essential oil formulation produced a 5mm clear zone of inhibition around the disks in one of the 63 samples evaluated. Pure (100%) tea tree essential oil formulation produced a 10mm clear zone of inhibition around the disks in four of the 63 samples evaluated, a 9mm zone in three samples, an 8mm zone in 16 samples, a 7mm zone in seven samples, a 6mm zone in two samples and there was no clear zone in 31 samples. Inhibition zones were produced by strains of Staphylococcus intermedius, Staphylococcus hyicus, Corynebacterium sp., Proteus mirabilis and Enterobacter sp. tea tree essential oil ear solution significantly induced remission of clinical signs both in bacterial and yeast ear infections. It also reduced as much Malassezia pachydermatis ear infection as the nystatin solution used in this study, while gentamycin solution showed better antibacterial effect. More studies should be conducted to evaluate in vitro diffusion properties of tea tree essential oil. Good antimicrobial spectrum and the absence of adverse reactions confirm the importance of developing a tea tree formulation as an alternative therapy for ear infections in dogs.
650 $aDogs
650 $aEar diseases
650 $aPhytotherapy
650 $aYeasts
650 $aBactéria
650 $aCão
650 $aFitoterapia
653 $aClínica
653 $aClinics
653 $aDoença de ouvido
653 $aLevedura
653 $aÓleo essencial de melaleuca
700 1 $aMAKINO, H.
700 1 $aCRUZ, T. P. P. S.
700 1 $aSILVEIRA, M. M.
700 1 $aSOUSA, V. R. F.
700 1 $aDUTRA, V.
700 1 $aLIMA, M. E. K. M.
700 1 $aBELLI, C. B.
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 38, n 8, p. 1597-1607, agosto 2018
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