| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Café. |
|
Data corrente: |
27/01/2021 |
|
Data da última atualização: |
27/01/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SILVA, J. O. da C. e; BRUCKNER, C. H.; CARNEIRO, P. C. S.; RESENDE, M. D. V. de; ALVES, R. S.; MATIAS, R. G. P. |
|
Afiliação: |
JOSÉ OSMAR DA COSTA E SILVA, UFV; CLAUDIO HORST BRUCKNER, UFV; PEDRO CRESCÊNCIO SOUZA CARNEIRO, UFV; MARCOS DEON VILELA DE RESENDE, CNPCa; RODRIGO SILVA ALVES, UFV; ROSANA GONÇALVES PIRES MATIAS, UFV. |
|
Título: |
Estimates of genetic parameters and repeatability coefficients in S2 peach genotypes grown in a subtropical environment. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Functional Plant Breeding Journal, v. 2, n. 1, 2020. p. 25-33. |
|
DOI: |
http://dx.doi.org/10.35418/2526-4117/v2n1a3 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Estimates of genetic parameters and repeatability coefficients in peach breeding are scarce. In view of this, the present study was carried out to estimate genetic parameters for plant and fruit traits and repeatability coefficients for fruit traits in S2 peach genotypes grown in a subtropical environment. A total of 434 individuals from 55 full-sib families of five populations were used in the study. The following plant traits were evaluated: trunk diameter at 10 cm above soil level (TD10), trunk diameter at 130 cm above soil level (TD130), plant height (PH), rate of budding on mixed branches (RB), rate of blind nodes on mixed branches (RBN), fruit number (FN) and harvest time (HT). In the fruit, the following traits were measured: weight (FW), diameter (FD), length (FL), diameter/length ratio (FD/FL), percentage of red in the epidermis (PRE), apex prominence (AP), suture line prominence (SLP), total soluble solids content (TSS), total titratable acidity (TTA) and TSS/TTA ratio (TSS/TTA). Repeated measures were performed for FD, FL, FD/FL, PRE, AP and SLP. Statistical analyses were run through the mixed model methodology. Genetic variability was detected for all traits. Individual narrowsense heritability values of moderate to high magnitude were found for the plant traits and of low to moderate magnitude for the fruit traits. Repeatability coefficients of moderate to high magnitude were obtained for the fruit traits. Except for the SLP trait, the evaluation of six fruits per plant revealed coefficients of determination higher than 80%, indicating high accuracy. MenosEstimates of genetic parameters and repeatability coefficients in peach breeding are scarce. In view of this, the present study was carried out to estimate genetic parameters for plant and fruit traits and repeatability coefficients for fruit traits in S2 peach genotypes grown in a subtropical environment. A total of 434 individuals from 55 full-sib families of five populations were used in the study. The following plant traits were evaluated: trunk diameter at 10 cm above soil level (TD10), trunk diameter at 130 cm above soil level (TD130), plant height (PH), rate of budding on mixed branches (RB), rate of blind nodes on mixed branches (RBN), fruit number (FN) and harvest time (HT). In the fruit, the following traits were measured: weight (FW), diameter (FD), length (FL), diameter/length ratio (FD/FL), percentage of red in the epidermis (PRE), apex prominence (AP), suture line prominence (SLP), total soluble solids content (TSS), total titratable acidity (TTA) and TSS/TTA ratio (TSS/TTA). Repeated measures were performed for FD, FL, FD/FL, PRE, AP and SLP. Statistical analyses were run through the mixed model methodology. Genetic variability was detected for all traits. Individual narrowsense heritability values of moderate to high magnitude were found for the plant traits and of low to moderate magnitude for the fruit traits. Repeatability coefficients of moderate to high magnitude were obtained for the fruit traits. Except for the SLP trait, the evaluation of six fruits p... Mostrar Tudo |
|
Thesagro: |
Melhoramento Genético Vegetal; Pêssego. |
|
Thesaurus Nal: |
Fruits; Peaches; Prunus persica var. nucipersica; Selection methods. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/220698/1/ESTIMATES-OF-GENETIC-PARAMETERS.pdf
|
|
Marc: |
LEADER 02457naa a2200265 a 4500
001 2129617
005 2021-01-27
008 2020 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.35418/2526-4117/v2n1a3$2DOI
100 1 $aSILVA, J. O. da C. e
245 $aEstimates of genetic parameters and repeatability coefficients in S2 peach genotypes grown in a subtropical environment.$h[electronic resource]
260 $c2020
520 $aEstimates of genetic parameters and repeatability coefficients in peach breeding are scarce. In view of this, the present study was carried out to estimate genetic parameters for plant and fruit traits and repeatability coefficients for fruit traits in S2 peach genotypes grown in a subtropical environment. A total of 434 individuals from 55 full-sib families of five populations were used in the study. The following plant traits were evaluated: trunk diameter at 10 cm above soil level (TD10), trunk diameter at 130 cm above soil level (TD130), plant height (PH), rate of budding on mixed branches (RB), rate of blind nodes on mixed branches (RBN), fruit number (FN) and harvest time (HT). In the fruit, the following traits were measured: weight (FW), diameter (FD), length (FL), diameter/length ratio (FD/FL), percentage of red in the epidermis (PRE), apex prominence (AP), suture line prominence (SLP), total soluble solids content (TSS), total titratable acidity (TTA) and TSS/TTA ratio (TSS/TTA). Repeated measures were performed for FD, FL, FD/FL, PRE, AP and SLP. Statistical analyses were run through the mixed model methodology. Genetic variability was detected for all traits. Individual narrowsense heritability values of moderate to high magnitude were found for the plant traits and of low to moderate magnitude for the fruit traits. Repeatability coefficients of moderate to high magnitude were obtained for the fruit traits. Except for the SLP trait, the evaluation of six fruits per plant revealed coefficients of determination higher than 80%, indicating high accuracy.
650 $aFruits
650 $aPeaches
650 $aPrunus persica var. nucipersica
650 $aSelection methods
650 $aMelhoramento Genético Vegetal
650 $aPêssego
700 1 $aBRUCKNER, C. H.
700 1 $aCARNEIRO, P. C. S.
700 1 $aRESENDE, M. D. V. de
700 1 $aALVES, R. S.
700 1 $aMATIAS, R. G. P.
773 $tFunctional Plant Breeding Journal$gv. 2, n. 1, 2020. p. 25-33.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Café (CNPCa) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Arroz e Feijão. |
|
Data corrente: |
13/10/2020 |
|
Data da última atualização: |
19/03/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
TEIXEIRA, N. C.; WENDLAND, A.; OLIVEIRA, M. I. de S.; BRANDÃO, L. T. D.; SOUZA, T. L. P. O. de; FARIA, J. C. de; DEL PELOSO, M. J.; CORTES, M. V. de C. B. |
|
Afiliação: |
NARA CRISTINA TEIXEIRA, bolsista CNPAF; ADRIANE WENDLAND FERREIRA, CNPAF; MAYTHSULENE INACIO DE SOUZA OLIVEIRA, bolsista CNPAF; LIVIA TEIXEIRA DUARTE BRANDAO, CNPAF; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; JOSIAS CORREA DE FARIA, CNPAF; MARIA JOSE DEL PELOSO, CNPAF; MARCIO VINICIUS DE C BARROS CORTES, CNPAF. |
|
Título: |
Rapid identification of RNA-interference-based resistance to Bean golden mosaic virus in transgenic common beans via loop-mediated isothermal amplification. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Crop Science, v. 60, n. 6, p. 3004-3012, Nov./Dec. 2020. |
|
ISSN: |
1435-0653 |
|
DOI: |
https://doi.org/10.1002/csc2.20107 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Amolecular toolwas adapted for practical identification of theworld?s first transgenic event (Embrapa 5.1) in common bean (Phaseolus vulgaris L.). Based on the technology of RNA interference and on the transformation of plants via the Biobalistic method, a transgenic bean was created with effective resistance to Bean golden mosaic virus, its principal virus. To support the monitoring of this technology, the development of new cultivars, and the process of producing, benefiting, and distributing of seeds and grains, a molecular detection tool based on loop-mediated isothermal amplification (LAMP) was developed. This tool can facilitating diagnosis, making it fast, specific, low-cost, and easily executed. Based on the gene Ahas (GenBank M88686) of the Embrapa 5.1 event, sets of initiators were planned (forward outer primer?backward outer primer and forward inner primer?backward inner primer) that are characteristic of the LAMP method. These were evaluated for their sensitivity and specificity in detecting their target. The LAMP method allows direct visual interpretation. The Neutral Red (NR) pH indicator was adopted to facilitate molecular diagnosis in moderately well equipped environments, thus reducing the risk of contamination and the need for skilled labor. This agility in diagnosis occurs because several phases commonly used inmolecular techniques, such as polymerase chain reaction, can be excluded. The LAMPmethod, combined with a rapid DNA extraction method (modified NaOH) and the addition of NR, provides technological support for addressing issues of biosafety, traceability, and identification of the Embrapa 5.1 event in beans in the field and in industry. MenosAmolecular toolwas adapted for practical identification of theworld?s first transgenic event (Embrapa 5.1) in common bean (Phaseolus vulgaris L.). Based on the technology of RNA interference and on the transformation of plants via the Biobalistic method, a transgenic bean was created with effective resistance to Bean golden mosaic virus, its principal virus. To support the monitoring of this technology, the development of new cultivars, and the process of producing, benefiting, and distributing of seeds and grains, a molecular detection tool based on loop-mediated isothermal amplification (LAMP) was developed. This tool can facilitating diagnosis, making it fast, specific, low-cost, and easily executed. Based on the gene Ahas (GenBank M88686) of the Embrapa 5.1 event, sets of initiators were planned (forward outer primer?backward outer primer and forward inner primer?backward inner primer) that are characteristic of the LAMP method. These were evaluated for their sensitivity and specificity in detecting their target. The LAMP method allows direct visual interpretation. The Neutral Red (NR) pH indicator was adopted to facilitate molecular diagnosis in moderately well equipped environments, thus reducing the risk of contamination and the need for skilled labor. This agility in diagnosis occurs because several phases commonly used inmolecular techniques, such as polymerase chain reaction, can be excluded. The LAMPmethod, combined with a rapid DNA extraction method (modified NaO... Mostrar Tudo |
|
Thesagro: |
DNA; Feijão; Mosaico Dourado; Phaseolus Vulgaris; Planta Transgênica. |
|
Thesaurus NAL: |
Bean golden mosaic virus; Beans; Loop-mediated isothermal amplification; RNA interference; Transgenic plants. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 02809naa a2200349 a 4500
001 2125441
005 2021-03-19
008 2020 bl uuuu u00u1 u #d
022 $a1435-0653
024 7 $ahttps://doi.org/10.1002/csc2.20107$2DOI
100 1 $aTEIXEIRA, N. C.
245 $aRapid identification of RNA-interference-based resistance to Bean golden mosaic virus in transgenic common beans via loop-mediated isothermal amplification.$h[electronic resource]
260 $c2020
520 $aAmolecular toolwas adapted for practical identification of theworld?s first transgenic event (Embrapa 5.1) in common bean (Phaseolus vulgaris L.). Based on the technology of RNA interference and on the transformation of plants via the Biobalistic method, a transgenic bean was created with effective resistance to Bean golden mosaic virus, its principal virus. To support the monitoring of this technology, the development of new cultivars, and the process of producing, benefiting, and distributing of seeds and grains, a molecular detection tool based on loop-mediated isothermal amplification (LAMP) was developed. This tool can facilitating diagnosis, making it fast, specific, low-cost, and easily executed. Based on the gene Ahas (GenBank M88686) of the Embrapa 5.1 event, sets of initiators were planned (forward outer primer?backward outer primer and forward inner primer?backward inner primer) that are characteristic of the LAMP method. These were evaluated for their sensitivity and specificity in detecting their target. The LAMP method allows direct visual interpretation. The Neutral Red (NR) pH indicator was adopted to facilitate molecular diagnosis in moderately well equipped environments, thus reducing the risk of contamination and the need for skilled labor. This agility in diagnosis occurs because several phases commonly used inmolecular techniques, such as polymerase chain reaction, can be excluded. The LAMPmethod, combined with a rapid DNA extraction method (modified NaOH) and the addition of NR, provides technological support for addressing issues of biosafety, traceability, and identification of the Embrapa 5.1 event in beans in the field and in industry.
650 $aBean golden mosaic virus
650 $aBeans
650 $aLoop-mediated isothermal amplification
650 $aRNA interference
650 $aTransgenic plants
650 $aDNA
650 $aFeijão
650 $aMosaico Dourado
650 $aPhaseolus Vulgaris
650 $aPlanta Transgênica
700 1 $aWENDLAND, A.
700 1 $aOLIVEIRA, M. I. de S.
700 1 $aBRANDÃO, L. T. D.
700 1 $aSOUZA, T. L. P. O. de
700 1 $aFARIA, J. C. de
700 1 $aDEL PELOSO, M. J.
700 1 $aCORTES, M. V. de C. B.
773 $tCrop Science$gv. 60, n. 6, p. 3004-3012, Nov./Dec. 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
|
|
| Registros recuperados : 2 | |
| 1. |  | 1600008, ACTA PATHOLOGICA ET MICROBIOLOGICA SCANDINAVICA . SECTION B , SUPPLEMENT, Scandinavian Societies for Medical Microbiology and Pathology, Copenhagen, DK
Biblioteca(s): Catálogo Coletivo de Periódicos Embrapa; Embrapa Acre; Embrapa Arroz e Feijão; Embrapa Cerrados; Embrapa Gado de Corte; Embrapa Hortaliças; Embrapa Pantanal; Embrapa Suínos e Aves; Embrapa Tabuleiros Costeiros; Embrapa Trigo; Embrapa Uva e Vinho. MenosCatálogo Coletivo de Periódicos Embrapa; Embrapa Acre; Embrapa Arroz e Feijão; Embrapa Cerrados; Embrapa Gado de Corte; Embrapa Hortaliças; Embrapa Pantanal; Embrapa Suínos e Aves; Embrapa Tabuleiros Costeiros; Embrapa Trigo... Mostrar Todas |   |
| Registros recuperados : 2 | |
|
|
|
