Data/hora: 08/06/2024 00:35:10

Biblioteca(s): | Embrapa Meio-Norte.
Data corrente: | 20/03/2012
Data da última atualização: | 02/06/2022
Autoria: | BRITTO, F. B.; D. S. F. MENDES; OGAWA, M.; CINTRA, I. H. A.; DINIZ, F. M.
Afiliação: | FABIO BARROS BRITTO, UFPI; MENDES, D. S. F., CPAMN; M. OGAWA, UFCE; ISRAEL HIDENBURGO ANICETO CINTRA, UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA; FABIO MENDONCA DINIZ, CPAMN.
Título: | Single primer-based DNA amplification as a suitable and low-cost tool for assessing genetic diversity in mangrove crabs.
Ano de publicação: | 2011
Fonte/Imprenta: | Genetics and Molecular Research, Ribeirão Preto, v. 10, n. 4, p. 4084-4092, 2011.
ISSN: | 1676-5680
Idioma: | Inglês
Conteúdo: | We used single primer-based DNA markers to assess genetic variability of the mangrove crab, Ucides cordatus, collected from four different localities from Pará to Santa Catarina States in Brazil (almost 5000 km distant). Five primers were chosen based on the consistency of the amplified bands and the polymorphism of each locus. A total of 78 loci were amplified in 76 samples; high polymorphism rates were detected in the entire sample (80.8%) and within each locality (73.5-79.5%). Analysis of molecular variance demonstrates significant differences between localities (P < 0.001); however, the &#934;ST value (0.078) indicates a low level of genetic differentiation, which suggests that U. cordatus larvae can spread over large distances. The variation was distributed among the samples, and most of it was attributed to differences among individuals within localities. Cluster analysis, based on the Jaccard similarity coefficient, and the Mantel test gave similar results to the analysis of molecular variance data. Despite the low level of population structuring, these markers could be used for studying U. cordatus diversity, due to the high level of polymorphism.
Palavras-Chave: | Diversidade.
Thesagro: | Caranguejo; Marcador Molecular.
Categoria do assunto: | G Melhoramento Genético
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/56104/1/SinglePrimerFabioDiniz.pdf
Registro original: | Embrapa Meio-Norte (CPAMN)
Exemplares: | CPAMN - (26771-1) - UPC - AP - PP - S 31/12 -  - 2012.00031 - DIS