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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
22/04/2010 |
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Data da última atualização: |
24/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CEOTTO, H.; NASCIMENTO, J. dos S.; BRITO, M. A. V. P. e; BASTOS, M. do C. de F. |
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Afiliação: |
HILANA CEOTTO, UFRJ; JANAÍNA DOS SANTOS NASCIMENTO, UFRJ; MARIA APARECIDA V PAIVA E BRITO, CNPGL; MARIA DO CARMO DE FREIRE BASTOS, UFRJ. |
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Título: |
Bacteriocin production by Staphylococcus aureus involved in bovine mastitis in Brazil. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Research in Microbiology, Paris, v. 160, p. 592-599, 2009. |
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DOI: |
https://doi.org/10.1016/j.resmic.2009.07.007 |
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Idioma: |
Inglês |
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Conteúdo: |
In the present study, 257 Staphylococcus spp. strains were isolated from bovine mastitis cases in 56 different Brazilian dairy herds located in the southeast region of the country and tested for antimicrobial substance (AMS) production. Forty-six strains (17.9%) exhibited AMS production and their identification as Staphylococcus aureus was based on the presence of Gram-positive cocci and on positive results in tests for the ability to coagulate rabbit plasma, to ferment mannitol, and to produce acetoin. The AMS were characterized as bacteriocins (Bac) by their sensitivity to proteolytic enzymes. The Bacþ strains were tested for resistance to 14 antimicrobial agents showing different profiles. Eighteen strains (39.0%) expressed a multiple antibiotic resistance phenotype. Forty-five strains exhibited at least one plasmid DNA. Cross-immunity analysis against strain S. aureus A70, which produces aureocin A70, amplification of the aurABCD operon (which encodes aureocin A70) or detection of this same operon by DNA/DNA hybridization revealed that 34 strains produce bacteriocins either identical or similar to aureocin A70. The remaining 12 Bacþ strains produce antimicrobial peptides that seem to be distinct from the best characterized staphylococcal bacteriocins described thus far. The bacteriocin produced by strain 4185 may possess potential practical applications, since it was able to inhibit important pathogens such as Bacillus cereus, Listeria monocytogenes, and Staphylococcus spp. isolated from nosocomial infections. MenosIn the present study, 257 Staphylococcus spp. strains were isolated from bovine mastitis cases in 56 different Brazilian dairy herds located in the southeast region of the country and tested for antimicrobial substance (AMS) production. Forty-six strains (17.9%) exhibited AMS production and their identification as Staphylococcus aureus was based on the presence of Gram-positive cocci and on positive results in tests for the ability to coagulate rabbit plasma, to ferment mannitol, and to produce acetoin. The AMS were characterized as bacteriocins (Bac) by their sensitivity to proteolytic enzymes. The Bacþ strains were tested for resistance to 14 antimicrobial agents showing different profiles. Eighteen strains (39.0%) expressed a multiple antibiotic resistance phenotype. Forty-five strains exhibited at least one plasmid DNA. Cross-immunity analysis against strain S. aureus A70, which produces aureocin A70, amplification of the aurABCD operon (which encodes aureocin A70) or detection of this same operon by DNA/DNA hybridization revealed that 34 strains produce bacteriocins either identical or similar to aureocin A70. The remaining 12 Bacþ strains produce antimicrobial peptides that seem to be distinct from the best characterized staphylococcal bacteriocins described thus far. The bacteriocin produced by strain 4185 may possess potential practical applications, since it was able to inhibit important pathogens such as Bacillus cereus, Listeria monocytogenes, and Staphylococcus s... Mostrar Tudo |
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Thesagro: |
Staphylococcus Aureus. |
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Thesaurus Nal: |
antimicrobial peptides; bacteriocins; bovine mastitis. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02247naa a2200217 a 4500
001 1697365
005 2023-03-24
008 2009 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.resmic.2009.07.007$2DOI
100 1 $aCEOTTO, H.
245 $aBacteriocin production by Staphylococcus aureus involved in bovine mastitis in Brazil.$h[electronic resource]
260 $c2009
520 $aIn the present study, 257 Staphylococcus spp. strains were isolated from bovine mastitis cases in 56 different Brazilian dairy herds located in the southeast region of the country and tested for antimicrobial substance (AMS) production. Forty-six strains (17.9%) exhibited AMS production and their identification as Staphylococcus aureus was based on the presence of Gram-positive cocci and on positive results in tests for the ability to coagulate rabbit plasma, to ferment mannitol, and to produce acetoin. The AMS were characterized as bacteriocins (Bac) by their sensitivity to proteolytic enzymes. The Bacþ strains were tested for resistance to 14 antimicrobial agents showing different profiles. Eighteen strains (39.0%) expressed a multiple antibiotic resistance phenotype. Forty-five strains exhibited at least one plasmid DNA. Cross-immunity analysis against strain S. aureus A70, which produces aureocin A70, amplification of the aurABCD operon (which encodes aureocin A70) or detection of this same operon by DNA/DNA hybridization revealed that 34 strains produce bacteriocins either identical or similar to aureocin A70. The remaining 12 Bacþ strains produce antimicrobial peptides that seem to be distinct from the best characterized staphylococcal bacteriocins described thus far. The bacteriocin produced by strain 4185 may possess potential practical applications, since it was able to inhibit important pathogens such as Bacillus cereus, Listeria monocytogenes, and Staphylococcus spp. isolated from nosocomial infections.
650 $aantimicrobial peptides
650 $abacteriocins
650 $abovine mastitis
650 $aStaphylococcus Aureus
700 1 $aNASCIMENTO, J. dos S.
700 1 $aBRITO, M. A. V. P. e
700 1 $aBASTOS, M. do C. de F.
773 $tResearch in Microbiology, Paris$gv. 160, p. 592-599, 2009.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
08/02/2017 |
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Data da última atualização: |
22/03/2017 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
CHAVES, N. P.; STAVER, C.; RODRIGUEZ, M. A. D. |
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Afiliação: |
N. P. CHAVES, RTB; C. STAVER, RTB; MIGUEL ANGEL DITA RODRIGUEZ, CNPMF. |
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Título: |
Potential of Trichoderma asperellum for biocontrol of Fusarium wilt in banana. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Acta Horticulturae, 1114, ISHS, 2016, |
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ISSN: |
0567-7572 |
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Idioma: |
Inglês |
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Conteúdo: |
A total of 72 fungal endophytes were isolated from surface-sterilized roots of healthy 'Gros Michel' (AAA genome, Gros Michel subgroup) banana plants in Turrialba, Costa Rica. In vitro and in vivo tests were carried out in order to identify microorganisms with potential activity against Fusarium oxysporum f. sp. cubense (Foc, race 1). In vitro tests revealed that 22 isolates inhibited the growth of Foc. The four most effective isolates were selected for suppression of Foc under greenhouse conditions. Plants treated with the four isolates showed a significant reduction of external and internal symptoms compared with untreated plants. Based on sequence analyses of specific genome regions [elongation factor - Tefa-1? or Internal Transcribed Spacer - ITS], the fungi tested in the greenhouse were identified as Trichoderma asperellum. Field trials are currently underway in Costa Rica to verify the performance of T. asperellum isolates as potential biological control agents against Foc. Results indicate that certain endophytic microorganisms may play important roles in controlling Fusarium wilt in banana. A better understanding of the antagonistic mechanisms displayed by endophytes in order to provide the best conditions to optimize the antagonistic activities of beneficial strains is still required. |
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Thesagro: |
Banana. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01773nam a2200157 a 4500
001 2063057
005 2017-03-22
008 2016 bl uuuu u00u1 u #d
022 $a0567-7572
100 1 $aCHAVES, N. P.
245 $aPotential of Trichoderma asperellum for biocontrol of Fusarium wilt in banana.$h[electronic resource]
260 $aActa Horticulturae, 1114, ISHS, 2016,$c2016
520 $aA total of 72 fungal endophytes were isolated from surface-sterilized roots of healthy 'Gros Michel' (AAA genome, Gros Michel subgroup) banana plants in Turrialba, Costa Rica. In vitro and in vivo tests were carried out in order to identify microorganisms with potential activity against Fusarium oxysporum f. sp. cubense (Foc, race 1). In vitro tests revealed that 22 isolates inhibited the growth of Foc. The four most effective isolates were selected for suppression of Foc under greenhouse conditions. Plants treated with the four isolates showed a significant reduction of external and internal symptoms compared with untreated plants. Based on sequence analyses of specific genome regions [elongation factor - Tefa-1? or Internal Transcribed Spacer - ITS], the fungi tested in the greenhouse were identified as Trichoderma asperellum. Field trials are currently underway in Costa Rica to verify the performance of T. asperellum isolates as potential biological control agents against Foc. Results indicate that certain endophytic microorganisms may play important roles in controlling Fusarium wilt in banana. A better understanding of the antagonistic mechanisms displayed by endophytes in order to provide the best conditions to optimize the antagonistic activities of beneficial strains is still required.
650 $aBanana
700 1 $aSTAVER, C.
700 1 $aRODRIGUEZ, M. A. D.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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