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Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
05/12/2016 |
Data da última atualização: |
05/12/2016 |
Autoria: |
SOUZA, F. N.; CUNHA, A. F.; ROSA, D. L. S. O.; BRITO, M. A. V. P. e; GUIMARAES, A. S.; MENDONCA, L. C.; SOUZA, G. N.; LAGE, A. P.; BLAGITZ, M. G.; DELLA LIBERA, A. M. M. P.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. |
Afiliação: |
FERNANDO N. SOUZA, UFMG; ADRIANO F. CUNHA, UFMG; DALILA L. S. O. ROSA, UFMG; MARIA APARECIDA V PAIVA E BRITO, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; LETICIA CALDAS MENDONCA, CNPGL; GUILHERME NUNES DE SOUZA, CNPGL; ANDREY P. LAGE, UFMG; MAIARA G. BLAGITZ, FMVZ/USP; ALICE M. M. P. DELLA LIBERA, FMVZ/USP; MARCOS B. HEINEMANN, FMVZ/USP; MÔNICA M. O. P. CERQUEIRA, UFMG. |
Título: |
Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF. V. 36, N. 9, p. 811-818, set. 2016. |
Idioma: |
Inglês |
Conteúdo: |
The most acceptable criteria for diagnosing bovine intramammary infections include results of bacteriological culture and measures of inflammation. Therefore, information on the diagnostic characteristics of the procedures used to identify infected quarters is required. Thus, this study was designed to evaluate a set of criteria to classify the infectious status of an udder at the quarter (single and duplicate milk samples) and cow (composite milk sample) levels, and to compare the infectious status with somatic cell counts (SCCs) of the samples. Here, the SCC thresholds determined by receiver operating characteristic curve analysis had a higher Youden index using mammary quarter duplicate milk samples as the gold standard for testing compared with single quarter and composite milk samples, especially for samples for which at least one of the duplicates was microbiologically positive, regardless of the mastitis pathogen isolated. The kappa coefficient for bacteriological results of the single quarter milk samples (single S1 and S2) was 0.85+0.019, indicating that single quarter milk sampling can be useful in mastitis control programs. Therefore, the use of composite milk samples to detect mastitis pathogens may be limited to the detection of major pathogens, given their predictive values. Thus, our findings suggest that the milk SCCs and microbiological examinations, although regarded as the most reliable indicators of ongoing mastitis, should be used in an integrated manner in mastitis control programs. Furthermore, the accuracy of single, duplicate and composite microbiological analyses to diagnosis mastitis should be considered for its implications in mastitis control strategies. MenosThe most acceptable criteria for diagnosing bovine intramammary infections include results of bacteriological culture and measures of inflammation. Therefore, information on the diagnostic characteristics of the procedures used to identify infected quarters is required. Thus, this study was designed to evaluate a set of criteria to classify the infectious status of an udder at the quarter (single and duplicate milk samples) and cow (composite milk sample) levels, and to compare the infectious status with somatic cell counts (SCCs) of the samples. Here, the SCC thresholds determined by receiver operating characteristic curve analysis had a higher Youden index using mammary quarter duplicate milk samples as the gold standard for testing compared with single quarter and composite milk samples, especially for samples for which at least one of the duplicates was microbiologically positive, regardless of the mastitis pathogen isolated. The kappa coefficient for bacteriological results of the single quarter milk samples (single S1 and S2) was 0.85+0.019, indicating that single quarter milk sampling can be useful in mastitis control programs. Therefore, the use of composite milk samples to detect mastitis pathogens may be limited to the detection of major pathogens, given their predictive values. Thus, our findings suggest that the milk SCCs and microbiological examinations, although regarded as the most reliable indicators of ongoing mastitis, should be used in an integrated manner... Mostrar Tudo |
Palavras-Chave: |
Especificidade; Infecção intramamária; Intramammary infection; Mastite; Saúde da glândula mamária; Sensibilidade; Sensitivity; Specificity; Udder health status. |
Thesagro: |
Mamite; Vaca leiteira. |
Thesaurus Nal: |
Dairy cows; Mastitis; Somatic cell count. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/151230/1/Somatic-cell-count-and-mastitis.pdf
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Marc: |
LEADER 02936naa a2200421 a 4500 001 2057934 005 2016-12-05 008 2016 bl uuuu u00u1 u #d 100 1 $aSOUZA, F. N. 245 $aSomatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples. 260 $c2016 520 $aThe most acceptable criteria for diagnosing bovine intramammary infections include results of bacteriological culture and measures of inflammation. Therefore, information on the diagnostic characteristics of the procedures used to identify infected quarters is required. Thus, this study was designed to evaluate a set of criteria to classify the infectious status of an udder at the quarter (single and duplicate milk samples) and cow (composite milk sample) levels, and to compare the infectious status with somatic cell counts (SCCs) of the samples. Here, the SCC thresholds determined by receiver operating characteristic curve analysis had a higher Youden index using mammary quarter duplicate milk samples as the gold standard for testing compared with single quarter and composite milk samples, especially for samples for which at least one of the duplicates was microbiologically positive, regardless of the mastitis pathogen isolated. The kappa coefficient for bacteriological results of the single quarter milk samples (single S1 and S2) was 0.85+0.019, indicating that single quarter milk sampling can be useful in mastitis control programs. Therefore, the use of composite milk samples to detect mastitis pathogens may be limited to the detection of major pathogens, given their predictive values. Thus, our findings suggest that the milk SCCs and microbiological examinations, although regarded as the most reliable indicators of ongoing mastitis, should be used in an integrated manner in mastitis control programs. Furthermore, the accuracy of single, duplicate and composite microbiological analyses to diagnosis mastitis should be considered for its implications in mastitis control strategies. 650 $aDairy cows 650 $aMastitis 650 $aSomatic cell count 650 $aMamite 650 $aVaca leiteira 653 $aEspecificidade 653 $aInfecção intramamária 653 $aIntramammary infection 653 $aMastite 653 $aSaúde da glândula mamária 653 $aSensibilidade 653 $aSensitivity 653 $aSpecificity 653 $aUdder health status 700 1 $aCUNHA, A. F. 700 1 $aROSA, D. L. S. O. 700 1 $aBRITO, M. A. V. P. e 700 1 $aGUIMARAES, A. S. 700 1 $aMENDONCA, L. C. 700 1 $aSOUZA, G. N. 700 1 $aLAGE, A. P. 700 1 $aBLAGITZ, M. G. 700 1 $aDELLA LIBERA, A. M. M. P. 700 1 $aHEINEMANN, M. B. 700 1 $aCERQUEIRA, M. M. O. P. 773 $tPesquisa Veterinária Brasileira, Brasília, DF. V. 36, N. 9, p. 811-818, set. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
25/03/2011 |
Data da última atualização: |
15/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
MOTA, G. B.; BATISTA, R. I. T. P.; SERAPIÃO, R. V.; BOITE, M. C.; VIANA, J. H. M. |
Afiliação: |
Gustavo Bruno Mota, UFV; RIBRIO IVAN TAVARES PEREIRA BATISTA, UNIVERSIDADE ESTADUAL DO CEARÁ; RAQUEL VARELA SERAPIAO, PESAGRO - RIO; MARIANA CORTES BOITE, UFF; JOAO HENRIQUE MOREIRA VIANA, CNPGL. |
Título: |
Developmental competence and expression of the MATER and ZAR1 genes in immature bovine oocytes selected by brilliant cresyl blue. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Zygote, v. 18, n. 3, p. 209-216, 2010. |
DOI: |
https://doi.org/10.1017/S0967199409990219 |
Idioma: |
Inglês |
Conteúdo: |
The objective of this work was to evaluate the selection of immature bovine oocytes by brilliant cresyl blue dye (BCB) and expression of transcripts MATER and ZAR1. Cumulus–oocyte complexes (COCs) from slaughterhouse ovaries were exposed to BCB diluted in mDPBS and incubated for 60 min at 38.5 °C in humidified air. After exposure those COCs were distributed in two groups, according to their cytoplasm colour: BCB+ (coloured cytoplasm) or BCB− (colourless cytoplasm). The control group was submitted to in vitro maturation (IVM) immediately after morphological selection and holding control group COCs were exposed to mDPBS without BCB but in the same incubation conditions of BCB+ and BCB− group. The COCs of all groups were submitted to IVM, in vitro fertilization (IVF) and in vitro culture (IVC). Cleavage rate (72 h post-insemination) was similar between control (65.3%) and BCB+ (64.4%) groups, but greater than (p < 0.05) holding control (49.8%) and BCB− (51.3%) groups. Blastocyst rate (192 h post-insemination) was not different between BCB+ (18.5%) and control (16.3%) groups, but greater (p < 0.05) than BCB− (8.4%) group. No difference was found for blastocyst rate between holding control group (14.2%), control and BCB+ groups. The relative expression of MATER and ZAR1 genes was evaluated by real-time PCR in immature oocytes collected from the control, holding control, BCB+ and BCB− groups. Despite the relative expression of MATER in holding control, BCB+ and BCB− were down regulated in comparison to control group there was no statistical difference (p > 0.05) in the relative expression of MATER and ZAR1 transcripts among groups. The results indicate that the BCB dye detects immature oocyte populations with different developmental competence, although no improvement in in vitro embryo production using oocytes exposed or not to BCB was observed. Development competence of immature oocytes exposed to BCB does not seem to be associated with variations in the expression of MATER and ZAR1 transcripts. MenosThe objective of this work was to evaluate the selection of immature bovine oocytes by brilliant cresyl blue dye (BCB) and expression of transcripts MATER and ZAR1. Cumulus–oocyte complexes (COCs) from slaughterhouse ovaries were exposed to BCB diluted in mDPBS and incubated for 60 min at 38.5 °C in humidified air. After exposure those COCs were distributed in two groups, according to their cytoplasm colour: BCB+ (coloured cytoplasm) or BCB− (colourless cytoplasm). The control group was submitted to in vitro maturation (IVM) immediately after morphological selection and holding control group COCs were exposed to mDPBS without BCB but in the same incubation conditions of BCB+ and BCB− group. The COCs of all groups were submitted to IVM, in vitro fertilization (IVF) and in vitro culture (IVC). Cleavage rate (72 h post-insemination) was similar between control (65.3%) and BCB+ (64.4%) groups, but greater than (p < 0.05) holding control (49.8%) and BCB− (51.3%) groups. Blastocyst rate (192 h post-insemination) was not different between BCB+ (18.5%) and control (16.3%) groups, but greater (p < 0.05) than BCB− (8.4%) group. No difference was found for blastocyst rate between holding control group (14.2%), control and BCB+ groups. The relative expression of MATER and ZAR1 genes was evaluated by real-time PCR in immature oocytes collected from the control, holding control, BCB+ and BCB− groups. Despite the relative expression of MATER in holding control, BCB+ and BCB− were down regu... Mostrar Tudo |
Palavras-Chave: |
Brilliant cresyl blue. |
Thesaurus NAL: |
gene expression; in vitro fertilization; oocytes. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02786naa a2200229 a 4500 001 1883077 005 2024-02-15 008 2010 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1017/S0967199409990219$2DOI 100 1 $aMOTA, G. B. 245 $aDevelopmental competence and expression of the MATER and ZAR1 genes in immature bovine oocytes selected by brilliant cresyl blue.$h[electronic resource] 260 $c2010 520 $aThe objective of this work was to evaluate the selection of immature bovine oocytes by brilliant cresyl blue dye (BCB) and expression of transcripts MATER and ZAR1. Cumulus–oocyte complexes (COCs) from slaughterhouse ovaries were exposed to BCB diluted in mDPBS and incubated for 60 min at 38.5 °C in humidified air. After exposure those COCs were distributed in two groups, according to their cytoplasm colour: BCB+ (coloured cytoplasm) or BCB− (colourless cytoplasm). The control group was submitted to in vitro maturation (IVM) immediately after morphological selection and holding control group COCs were exposed to mDPBS without BCB but in the same incubation conditions of BCB+ and BCB− group. The COCs of all groups were submitted to IVM, in vitro fertilization (IVF) and in vitro culture (IVC). Cleavage rate (72 h post-insemination) was similar between control (65.3%) and BCB+ (64.4%) groups, but greater than (p < 0.05) holding control (49.8%) and BCB− (51.3%) groups. Blastocyst rate (192 h post-insemination) was not different between BCB+ (18.5%) and control (16.3%) groups, but greater (p < 0.05) than BCB− (8.4%) group. No difference was found for blastocyst rate between holding control group (14.2%), control and BCB+ groups. The relative expression of MATER and ZAR1 genes was evaluated by real-time PCR in immature oocytes collected from the control, holding control, BCB+ and BCB− groups. Despite the relative expression of MATER in holding control, BCB+ and BCB− were down regulated in comparison to control group there was no statistical difference (p > 0.05) in the relative expression of MATER and ZAR1 transcripts among groups. The results indicate that the BCB dye detects immature oocyte populations with different developmental competence, although no improvement in in vitro embryo production using oocytes exposed or not to BCB was observed. Development competence of immature oocytes exposed to BCB does not seem to be associated with variations in the expression of MATER and ZAR1 transcripts. 650 $agene expression 650 $ain vitro fertilization 650 $aoocytes 653 $aBrilliant cresyl blue 700 1 $aBATISTA, R. I. T. P. 700 1 $aSERAPIÃO, R. V. 700 1 $aBOITE, M. C. 700 1 $aVIANA, J. H. M. 773 $tZygote$gv. 18, n. 3, p. 209-216, 2010.
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