Data/hora: 07/06/2024 18:24:00

Biblioteca(s): | Embrapa Suínos e Aves; Embrapa Unidades Centrais.
Data corrente: | 31/05/2016
Data da última atualização: | 08/11/2016
Autoria: | KUNERT-FILHO, H. C.; CIBULSKI, S. P.; FINKLER, F.; GRASSOTTI, T. T.; JAENISCH, F. R. F.; BRITO, K. C. T. de; CARVALHO, D.; LOVATO, M.; BRITO, B. G. de.
Afiliação: | HIRAN C. KUNERT-FILHO, Instituto de Pesquisas Veterinárias Desidério Finamor; SAMUEL PAULO CIBULSKI, Instituto de Pesquisas Veterinárias Desidério Finamor; FABRINE FINKLER, Instituto de Pesquisas Veterinárias Desidério Finamor; TIELA T. GRASSOTTI, Instituto de Pesquisas Veterinárias Desidério Finamor; FATIMA REGINA FERREIRA JAENISCH, CNPSA; KELLY CRISTINA TAGLIARI DE BRITO, Instituto de Pesquisas Veterinárias Desidério Finamor; DAIANE CARVALHO, Instituto de Pesquisas Veterinárias Desidério Finamor; MARISTELA LOVATO, UFSM; BENITO GUIMARÃES DE BRITO, Instituto de Pesquisas Veterinárias Desidério Finamor.
Título: | First phylogenetic analysis of Avipoxvirus (APV) in Brazil.
Ano de publicação: | 2016
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Brasília, DF, v. 36, n. 5, p. 357-362, maio. 2016.
Idioma: | Inglês

Conteúdo:

Abstract: This study represents the first phylogenetic analysis of avian poxvirus recovered from
turkeys in Brazil. The clinical disorders related to fowlpox herein described occurred in
a turkey housing system. The birds displaying characteristic pox lesions which were observed
on the neck, eyelids and beak of the turkeys. Four affected turkeys were randomly
chosen, euthanized and necropsied. Tissues samples were submitted for histopathological
analysis and total DNA was further extracted, amplified by conventional PCR, sequenced
and phylogenetically analyzed. Avian poxviruses specific PCR was performed based on P4b
core protein gene sequence. The histological analysis revealed dermal inflammatory process,
granulation tissue, hyperplasia of epithelial cells and inclusion bodies. The P4b gene
was detected in all samples. Sequencing revealed a 100% nucleotide and amino acid sequence
identity among the samples, and the sequences were deposited in GenBank®. The
four Avian poxviruses fragments sequenced in this study clustered along the A1 clade of
avipoxviruses, and were classified as Avipoxvirus (APV). Additional studies, such as virus
isolation, PCR and sequencing includinga large number of specimens from the Brazilian
turkey production must be conducted due to the hazardous risk that poxvirus infections
may cause to the Brazilian poultry production scenario, given that Brazil?s turkey production
attracts attention due to its economic importance worldwide. Our findings point to
the need to identify the prevalence of APV in Brazilian turkey production, to perform risk
assessment studies and continued surveillance of APV infections in both wild and commercial
avian species.

Palavras-Chave: | Análise filogenética; APV; Brasil; PCR; Turkey.
Thesagro: | Avicultura; Peru; Virologia.
Thesaurus Nal: | Acyrthosiphon pisum virus; Avipoxvirus; Brazil; Phylogeny; Poxviridae; Turkeys.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/143553/1/First-phylogenetic.pdf
Registro original: | Embrapa Suínos e Aves (CNPSA)

Exemplares:

AI-SEDE - (59554-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS
CNPSA - (20678-1) - UPC - AP - DD -  -  -  - DIS