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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
05/08/2009 |
Data da última atualização: |
30/05/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FIGUEIREDO, J. E. F.; GOMES, E. A.; GUIMARAES, C. T.; LANA, U. G. de P.; TEIXEIRA, M. A.; LIMA, G. V. C.; BRESSAN, W. |
Afiliação: |
JOSE EDSON FONTES FIGUEIREDO, CNPMS; ELIANE APARECIDA GOMES, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; Maria Aparecida Teixeira, CNPMS; Guilherme Vitor Correa Lima, Fundação Educacional Monsenhor Messias; WELLINGTON BRESSAN, CNPMS. |
Título: |
Molecular analysis endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.). |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 40, n. 3, p. 522-534, 2009. |
DOI: |
10.1590/S1517-83822009000300014 |
Idioma: |
Inglês |
Conteúdo: |
Endophytíc bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of whole-cell protein extract of forty-two isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some high-accumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosonal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains. Among the forty-two isolates identified by rDNA sequencing. Bacillus. subitilis and B. pumilus were the most frequenty species (15 and 12 isolates. respectively) followed by B. licheniforms (7 isolates), B. cereus (5 isolates) and B. uniloliquefascelns (3 isolates). According to present results. SDS-P AGE techínique could be used as a fast and cheap first tool for identifying inter-specific variation in maize endophytic bacterial collections while rDNA sequencing could be applied for analyzing intra-specific variation among isolates with similar protein profile as well as for taxonomic studies. |
Palavras-Chave: |
Bacillus; Endophytíc bacteria; rDNA sequencing; SDS-PAGE; Sweet corn. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/31303/1/Molecular-analysis.pdf
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Marc: |
LEADER 02190naa a2200265 a 4500 001 1488802 005 2018-05-30 008 2009 bl uuuu u00u1 u #d 024 7 $a10.1590/S1517-83822009000300014$2DOI 100 1 $aFIGUEIREDO, J. E. F. 245 $aMolecular analysis endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.).$h[electronic resource] 260 $c2009 520 $aEndophytíc bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of whole-cell protein extract of forty-two isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some high-accumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosonal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains. Among the forty-two isolates identified by rDNA sequencing. Bacillus. subitilis and B. pumilus were the most frequenty species (15 and 12 isolates. respectively) followed by B. licheniforms (7 isolates), B. cereus (5 isolates) and B. uniloliquefascelns (3 isolates). According to present results. SDS-P AGE techínique could be used as a fast and cheap first tool for identifying inter-specific variation in maize endophytic bacterial collections while rDNA sequencing could be applied for analyzing intra-specific variation among isolates with similar protein profile as well as for taxonomic studies. 653 $aBacillus 653 $aEndophytíc bacteria 653 $arDNA sequencing 653 $aSDS-PAGE 653 $aSweet corn 700 1 $aGOMES, E. A. 700 1 $aGUIMARAES, C. T. 700 1 $aLANA, U. G. de P. 700 1 $aTEIXEIRA, M. A. 700 1 $aLIMA, G. V. C. 700 1 $aBRESSAN, W. 773 $tBrazilian Journal of Microbiology, São Paulo$gv. 40, n. 3, p. 522-534, 2009.
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Embrapa Milho e Sorgo (CNPMS) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
19/08/2016 |
Data da última atualização: |
20/09/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
NOGUEIRA, D. M.; GARDINER, C. P.; GUMMOW, B.; CAVALIERI, J.; FITZPATRICK, L. A.; PARKER, A. J. |
Afiliação: |
DANIEL MAIA NOGUEIRA, CPATSA; C. P. GARDINER, School of Veterinary and Biomedical Sciences, James Cook University, Townsville; B. GUMMOW, School of Veterinary and Biomedical Sciences, James Cook University, Townsville; J. CAVALIERI, School of Veterinary and Biomedical Sciences, James Cook University, Townsville; L. A. FITZPATRICK, School of Veterinary and Biomedical Sciences, James Cook University, Townsville; A. J. PARKER, School of Veterinary and Biomedical Sciences, James Cook University, Townsville. |
Título: |
A survey of the meat goat industry in Queensland and New South Wales. 1. General property information, goat and pasture management. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Animal Production Science, v. 56, p. 1520-1532, 2016. |
DOI: |
10.1071/AN14793 |
Idioma: |
Inglês |
Conteúdo: |
This study aimed to survey farmers knowledge and practices on the management of pastures, stocking rates and markets of meat goat-producing enterprises within New South Wales and Queensland, Australia. An interview-based questionnaire was conducted on properties that derived a significant proportion of their income from goats. The survey covered 31 landholders with a total land area of 567 177 ha and a reported total of 160 010 goats. A total of 55% (17/31) of producers were involved in both opportunistic harvesting and commercial goat operations, and 45% (14/31) were specialised seedstock producers. Goats were the most important livestock enterprise on 55% (17/31) of surveyed properties. Stocking rate varied considerably (0.3?9.3 goats/ha) within and across surveyed properties and was found to be negatively associated with property size and positively associated with rainfall. Overall, 81% (25/31) of producers reported that the purpose of running goats on their properties was to target international markets. Producers also cited the importance of targeting markets as a way to increase profitability. Fifty-three percent of producers were located over 600 km from a processing plant and the high cost of freight can limit the continuity of goats supplied to abattoirs. Fencing was an important issue for goat farmers, with many producers acknowledging this could potentially add to capital costs associated with better goat management and production. Producers in the pastoral regions appear to have a low investment in pasture development and opportunistic goat harvesting appears to be an important source of income. MenosThis study aimed to survey farmers knowledge and practices on the management of pastures, stocking rates and markets of meat goat-producing enterprises within New South Wales and Queensland, Australia. An interview-based questionnaire was conducted on properties that derived a significant proportion of their income from goats. The survey covered 31 landholders with a total land area of 567 177 ha and a reported total of 160 010 goats. A total of 55% (17/31) of producers were involved in both opportunistic harvesting and commercial goat operations, and 45% (14/31) were specialised seedstock producers. Goats were the most important livestock enterprise on 55% (17/31) of surveyed properties. Stocking rate varied considerably (0.3?9.3 goats/ha) within and across surveyed properties and was found to be negatively associated with property size and positively associated with rainfall. Overall, 81% (25/31) of producers reported that the purpose of running goats on their properties was to target international markets. Producers also cited the importance of targeting markets as a way to increase profitability. Fifty-three percent of producers were located over 600 km from a processing plant and the high cost of freight can limit the continuity of goats supplied to abattoirs. Fencing was an important issue for goat farmers, with many producers acknowledging this could potentially add to capital costs associated with better goat management and production. Producers in the pastoral regio... Mostrar Tudo |
Palavras-Chave: |
Cabras selvagens; Carne de caprino. |
Thesagro: |
Caprino; Mercado; Pastagem; Produção animal. |
Thesaurus NAL: |
Animal production. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02466naa a2200277 a 4500 001 2051315 005 2018-09-20 008 2016 bl uuuu u00u1 u #d 024 7 $a10.1071/AN14793$2DOI 100 1 $aNOGUEIRA, D. M. 245 $aA survey of the meat goat industry in Queensland and New South Wales. 1. General property information, goat and pasture management.$h[electronic resource] 260 $c2016 520 $aThis study aimed to survey farmers knowledge and practices on the management of pastures, stocking rates and markets of meat goat-producing enterprises within New South Wales and Queensland, Australia. An interview-based questionnaire was conducted on properties that derived a significant proportion of their income from goats. The survey covered 31 landholders with a total land area of 567 177 ha and a reported total of 160 010 goats. A total of 55% (17/31) of producers were involved in both opportunistic harvesting and commercial goat operations, and 45% (14/31) were specialised seedstock producers. Goats were the most important livestock enterprise on 55% (17/31) of surveyed properties. Stocking rate varied considerably (0.3?9.3 goats/ha) within and across surveyed properties and was found to be negatively associated with property size and positively associated with rainfall. Overall, 81% (25/31) of producers reported that the purpose of running goats on their properties was to target international markets. Producers also cited the importance of targeting markets as a way to increase profitability. Fifty-three percent of producers were located over 600 km from a processing plant and the high cost of freight can limit the continuity of goats supplied to abattoirs. Fencing was an important issue for goat farmers, with many producers acknowledging this could potentially add to capital costs associated with better goat management and production. Producers in the pastoral regions appear to have a low investment in pasture development and opportunistic goat harvesting appears to be an important source of income. 650 $aAnimal production 650 $aCaprino 650 $aMercado 650 $aPastagem 650 $aProdução animal 653 $aCabras selvagens 653 $aCarne de caprino 700 1 $aGARDINER, C. P. 700 1 $aGUMMOW, B. 700 1 $aCAVALIERI, J. 700 1 $aFITZPATRICK, L. A. 700 1 $aPARKER, A. J. 773 $tAnimal Production Science$gv. 56, p. 1520-1532, 2016.
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