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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Instrumentação. |
Data corrente: |
28/09/2022 |
Data da última atualização: |
28/09/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
COATRINI-SOARES, A.; COATRINI-SOARES, J.; POPOLIN NETO, M.; MELLO, S. S. de; PINTO, D. S. C.; CARVALHO, W. A.; GILMORE, M. S.; PIAZZETTA, M. H. O.; GOBBI, A. L.; BRANDÃO, H. M.; PAULOVICH, F. V.; OLIVEIRA JR, O. N.; MATTOSO, L. H. C. |
Afiliação: |
LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA. |
Título: |
Microfluidic E-tongue to diagnose bovine mastitis with milk samples using machine learning with decision tree models. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Chemical Engineering Journal, v. 451, e138523, 2022. |
Páginas: |
1 - 9 |
ISSN: |
1385-8947 |
DOI: |
https://doi.org/10.1016/j.cej.2022.138523 |
Idioma: |
Inglês |
Conteúdo: |
We report an electronic tongue based on impedance spectroscopy to detect Staphylococcus aureus and diagnose bovine mastitis in milk samples. This was achieved with optimized sensing units made with layer-by-layer films and by treating the capacitance data with machine learning algorithms employing decision trees models. These films were made with chitosan, chondroitin sulfate, sericin and gold nanoparticles /sericin, whose molecularlevel interaction with S.aureus depended on the architecture according to PM-IRRAS measurements. The limit of detection in blank milk varied from 3.41 to 2.01 CFU/mL depending on the sensing unit. This sensitivity was complemented with the selectivity provided by combining the electrical responses of the four sensing units. Indeed, with machine learning it was possible to determine multidimensional calibration spaces (MCS) that could generate rules to explain how the milk samples could be discriminated. With a 7-dimension MCS, distinct S. aureus concentrations could be distinguished from possible interferents with a 100 % accuracy. In crude milk samples, 94 % accuracy was obtained with a 6-dimension MCS in multiclass classification for milk from different udders of a mastitis infected cow, including samples diluted 50-fold, in addition to milk from an infected cow treated with Bronopol and from a healthy cow. It is significant that in a ternary classification with these crude milk samples, a 2-dimension MCS could distinguish between milk from an infected cow, treated with Bronopol and from a healthy cow with 100 % accuracy. The combination of electronic tongues and machine learning ? as in this proof-of-concept study - is promising for diagnosis of mastitis at a low cost. MenosWe report an electronic tongue based on impedance spectroscopy to detect Staphylococcus aureus and diagnose bovine mastitis in milk samples. This was achieved with optimized sensing units made with layer-by-layer films and by treating the capacitance data with machine learning algorithms employing decision trees models. These films were made with chitosan, chondroitin sulfate, sericin and gold nanoparticles /sericin, whose molecularlevel interaction with S.aureus depended on the architecture according to PM-IRRAS measurements. The limit of detection in blank milk varied from 3.41 to 2.01 CFU/mL depending on the sensing unit. This sensitivity was complemented with the selectivity provided by combining the electrical responses of the four sensing units. Indeed, with machine learning it was possible to determine multidimensional calibration spaces (MCS) that could generate rules to explain how the milk samples could be discriminated. With a 7-dimension MCS, distinct S. aureus concentrations could be distinguished from possible interferents with a 100 % accuracy. In crude milk samples, 94 % accuracy was obtained with a 6-dimension MCS in multiclass classification for milk from different udders of a mastitis infected cow, including samples diluted 50-fold, in addition to milk from an infected cow treated with Bronopol and from a healthy cow. It is significant that in a ternary classification with these crude milk samples, a 2-dimension MCS could distinguish between milk from an i... Mostrar Tudo |
Palavras-Chave: |
Impedance spectroscopy; Machine learning; Mastite; Multidimensional calibration space; S aureus; Sensors. |
Thesagro: |
Bovino; Doença Animal; Gado Leiteiro. |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02936naa a2200409 a 4500 001 2146958 005 2022-09-28 008 2022 bl uuuu u00u1 u #d 022 $a1385-8947 024 7 $ahttps://doi.org/10.1016/j.cej.2022.138523$2DOI 100 1 $aCOATRINI-SOARES, A. 245 $aMicrofluidic E-tongue to diagnose bovine mastitis with milk samples using machine learning with decision tree models.$h[electronic resource] 260 $c2022 300 $a1 - 9 520 $aWe report an electronic tongue based on impedance spectroscopy to detect Staphylococcus aureus and diagnose bovine mastitis in milk samples. This was achieved with optimized sensing units made with layer-by-layer films and by treating the capacitance data with machine learning algorithms employing decision trees models. These films were made with chitosan, chondroitin sulfate, sericin and gold nanoparticles /sericin, whose molecularlevel interaction with S.aureus depended on the architecture according to PM-IRRAS measurements. The limit of detection in blank milk varied from 3.41 to 2.01 CFU/mL depending on the sensing unit. This sensitivity was complemented with the selectivity provided by combining the electrical responses of the four sensing units. Indeed, with machine learning it was possible to determine multidimensional calibration spaces (MCS) that could generate rules to explain how the milk samples could be discriminated. With a 7-dimension MCS, distinct S. aureus concentrations could be distinguished from possible interferents with a 100 % accuracy. In crude milk samples, 94 % accuracy was obtained with a 6-dimension MCS in multiclass classification for milk from different udders of a mastitis infected cow, including samples diluted 50-fold, in addition to milk from an infected cow treated with Bronopol and from a healthy cow. It is significant that in a ternary classification with these crude milk samples, a 2-dimension MCS could distinguish between milk from an infected cow, treated with Bronopol and from a healthy cow with 100 % accuracy. The combination of electronic tongues and machine learning ? as in this proof-of-concept study - is promising for diagnosis of mastitis at a low cost. 650 $aBovino 650 $aDoença Animal 650 $aGado Leiteiro 653 $aImpedance spectroscopy 653 $aMachine learning 653 $aMastite 653 $aMultidimensional calibration space 653 $aS aureus 653 $aSensors 700 1 $aCOATRINI-SOARES, J. 700 1 $aPOPOLIN NETO, M. 700 1 $aMELLO, S. S. de 700 1 $aPINTO, D. S. C. 700 1 $aCARVALHO, W. A. 700 1 $aGILMORE, M. S. 700 1 $aPIAZZETTA, M. H. O. 700 1 $aGOBBI, A. L. 700 1 $aBRANDÃO, H. M. 700 1 $aPAULOVICH, F. V. 700 1 $aOLIVEIRA JR, O. N. 700 1 $aMATTOSO, L. H. C. 773 $tChemical Engineering Journal$gv. 451, e138523, 2022.
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Registros recuperados : 29 | |
2. | | PASCHOALIN, R. T.; BRANDÃO, H. M.; ARAÚJO-MOREIRA, F. M.; HERRMANN, P. S. P. Agriculture applications of drug-delivery systems based on ferromagnetic graphite In: INTERNATIONAL CONFERENCE ON FOOD AND AGRICULTURE APPLICATIONS OF NANOTECHNOLOGIES - NanoAgri, 2010, São Pedro, SP. [Anais…] São Pedro: Aptor Software, 2010. Editors: Caue Ribeiro, Odílio Benedito Garrido de Assis, Luiz Henrique Capparelli Mattoso, Sérgio Mascarenhas.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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4. | | ZANETTE, R.; ALMEIDA, L. B. F.; ALMEIDA, C. G.; OLIVEIRA, C. R. de; BRANDÃO, H. M.; MUNK, M. Análise da citoxicidade in vitro das nanofibras de celulose de algodão. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 9., 2017, São Carlos. Anais ... São Carlos: Embrapa Instrumentação, 2017. p.681-684. Editores: Caue Ribeiro de Oliveira, Elaine Cristina Paris, Luiz Henrique Capparelli Mattoso, Marcelo Porto Bemquerer, Maria Alice Martins, Odílio Benedito Garrido de Assis.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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5. | | BRANDÃO, H. M.; SOUSA, R. V.; WOUTERS, F.; PADUA, L. V. R.; MADUREIRA, E. H.; TRALDI, A. S. Avaliação da influência da insulina no processo de luteogênese de ovinos. In: SIMPÓSIO E PÓS-GRADUAÇÃO DO DEPARTAMENTO DE REPRODUÇÃO ANIMAL, 2., 2008, Pirassununga. Anais... Pirassununga: USP, 2008. p. 43Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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8. | | BRANDAO, H. M.; TEIXEIRA, E. M.; ASSUNÇAO, C. M.; CARNEIRO, J.; MARCONCINI, J. M.; MATTOSO, L. H. C. Characterization of polycaprolactone (PCL) after treatment into ruminant digestive system. In: FRENCH-BRAZILIAN MEETING ON POLYMERS - FBPOL, 2., 2008, Florianópolis, SC. Abstracts? Florianópolis: Universidade Federal de Santa Catarina/Centre de Recherches sur les Macromolecules Vegetables, 2008. C35.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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9. | | ZAFALON, L. F.; SANTANA, R. C. M.; PILON, L. E.; BRANDÃO, H. M.; MOSQUEIRA, V. C. F.; TANAKA, E. V. Use of nanoparticulate and convencional antimicrobials for treatment of subclinical mastitis in sheep at drying off. In: WORLD VETERINARY CONGRESS , 31., 2013, Prague. Proceedings... Prague WVA, 2013.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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10. | | ZAFALON, L. F.; SANTANA, R. C. M.; PILON, L. E.; BRANDÃO, H. M.; MOSQUEIRA, V. C. F.; TANAKA, E. V. Use of nanoparticulate and convencional antimicrobials for treatment of subclinical in sheep at drying off. WORLD VETERINARY CONGRESS, 31., 2013, Prague. Proceedings... Prague: World Veterinary Association, 2013.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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12. | | BRANDÃO, H. M.; MARCONCINI, J. M.; SILVA, S. R. da; BARBOSA, N. R.; TRALDI, A. S.; MADUREIRA, E. H. A potential device for oral vaccination of ruminants In: INTERNATIONAL CONFERENCE ON FOOD AND AGRICULTURE APPLICATIONS OF NANOTECHNOLOGIES - NanoAgri, 2010, São Pedro, SP. [Anais…] São Pedro: Aptor Software, 2010. Editors: Caue Ribeiro, Odílio Benedito Garrido de Assis, Luiz Henrique Capparelli Mattoso, Sérgio Mascarenhas.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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13. | | PEREIRA, M. M.; MOUTON, L.; YEPREMIAN, C.; COUTE, A.; LO, J.; MARCONCINI, J. M.; LADEIRA, L. O.; RAPOSO, N. R. B.; BRANDAO, H. M.; BRAYNER, R. Interaction of carbon nanotube and cellulose nanofiber with algal cells klebsormidium flaccidum. In: INTERNATIONAL CONFERENCE ON SAFE PRODUCTION AND USE OF NANOMATERIALS - NANOSAFE, 4., 2014, Grenoble. Book of Abstracts... [S. l.: s. n.], 2014. P5-11.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite; Embrapa Instrumentação. |
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14. | | SIQUEIRA, M. C.; AOUADA, M. R. M.; CASTRO, V. L. S.; BRANDÃO, H. M.; RECH, R. R.; MARCONCINI, J. M.; MATTOSO, L. H. C. Caracterização e avaliação da toxicidade de nanopartículas de prata para incorporação em matriz polimérica para uso em embalagens de alimentos. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 7.; ESCOLA DE NANOTECNOLOGIA, 3., 2013, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação, 2013. p. 542-544 Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso. CD-ROM. Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite; Embrapa Instrumentação. |
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15. | | SOUZA, N. L. G. D. de; BRANDÃO, H. M.; CARNEIRO, J. C.; MARCONCINI, J. M.; DUQUE, A. C. A.; OLIVEIRA, L. F. C. de. Studying the possible application of chitosan in the formulation of bovine rumen delivery systems In: INTERNATIONAL CONFERENCE ON FOOD AND AGRICULTURE APPLICATIONS OF NANOTECHNOLOGIES - NanoAgri, 2010, São Pedro, SP. [Anais…] São Pedro: Aptor Software, 2010. Editors: Caue Ribeiro, Odílio Benedito Garrido de Assis, Luiz Henrique Capparelli Mattoso, Sérgio Mascarenhas.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite; Embrapa Instrumentação. |
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17. | | NOBRE, M. M.; CAMARGO, M. P. E.; MOURA, I. U. G.; ROCHA, D. T.; GERN, J. C.; MATTOSO, L. H. C.; RIBEIRO, C.; BRANDÃO, H. M. O uso do estudo de mercado como ferramenta decisória no processo de patenteabilidade internacional da tecnologia "nanopropolis". In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 6., 2012, Fortaleza. Anais... São Carlos: Embrapa Instrumentação; Fortaleza: Embrapa Agroindústria Tropical, 2012. p. 522-524. Editores: Maria Alice Martins, MOrsyleide de Freitas Rosa, Men de Sá Moreira de Souza Filho, Nicodemos Moreira dos Santos Junior, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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18. | | DA SILVA, S. R.; RAPOSO, N. R. B.; LANGE, C. C.; GERN, J. C.; BRANDI, R. R.; MATTOSO, L. H. C.; OLIVEIRA, C.; BRANDÃO, H. M. Uso de nanopartículas de própolis para encapsular azitromicina. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 6., 2012, Fortaleza. Anais... São Carlos: Embrapa Instrumentação; Fortaleza: Embrapa Agroindústria Tropical, 2012. p. 356-358. Editores: Maria Alice Martins, MOrsyleide de Freitas Rosa, Men de Sá Moreira de Souza Filho, Nicodemos Moreira dos Santos Junior, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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19. | | MUNK, M.; BRANDÃO, H. M.; NOWAK, S.; MOUTON, L.; GERN, J. C.; GUIMARÃES, A. S.; YÉPRÉMIAN, C.; COUTÉ, A.; RAPOSO, N. R. B.; MARCONCINI, J. M.; BRAYNER, R. Direct and indirect toxic effects of cotton-derived cellulose nanofibers on filamentous green algae. Ecotoxicology and environmental safety, v. 122, p. 399-405, 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Instrumentação. |
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20. | | PEREIRA, M. M; MOUTON, L.; YÉPRÉMIAN, C.; COUTÉ, A.; LO, J.; MARCONCINI, J. M.; LADEIRA, L. O.; RAPOSO, N. RB; BRANDÃO, H. M.; BRAYNER, R. Ecotoxicological effects of carbon nanotubes and cellulose nanofibers in Chlorella vulgaris. Journal of Nanobiotechnology, Londres, v. 12, n. 15, p. 1-13, 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Gado de Leite; Embrapa Instrumentação. |
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Registros recuperados : 29 | |
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