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| Registros recuperados : 9 | |
| 2. |  | AGUIAR, M. de J. N.; SOUSA NETO, N. C. de; BRAGA, C. C. : BRITO, J. I. B. de; SILVA, E. D. V.; SILVA, F. B. R.; BURGOS, N.; VAREJÃO-SILVA, M. A.; COSTA, C. A. R. da. Zoneamento pedoclimático do cajueiro (Anacardium occidentale L.) no Nordeste do Brasil e Norte de Minas Gerais. Fortaleza: Embrapa Agroindústria Tropical; Recife: Embrapa-CNPS-ERP-NE, 2000. 30 p. (Embrapa Agroindústria Tropical. Boletim de Pesquisa, 27). Anexo mapa na escala 1:2.000.000.| Biblioteca(s): Embrapa Meio-Norte; Embrapa Roraima. |
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| 3. | | AGUIAR, M. de J. N.; SOUSA NETO, N. C. de; BRAGA, C. C.; BRITO, J. I. B. de; SILVA, E. D. V.; SILVA, F. B. R.; BURGOS, N.; VAREJAO-SILVA, M. A. Zoneamento pedoclimático para a cultura do cajueiro (Anacardium occidentale L.) no Nordeste do Brasil e Norte de Minas Gerais. Fortaleza, CE: Embrapa-CNPAT; Recife, PE: Embrapa-CNPS-ERP, 1999. 26 p. (Embrapa. CNPAT. Boletim de Pesquisa, 27).| Biblioteca(s): Embrapa Solos / UEP-Recife. |
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| 5. | | AGUIAR, M. de J. N.; SOUZA NETO, N. C. de; BRAGA, C. C.; BRITO, J. I. B. de; SILVA, E. D. V.; SILVA, F. B. R.; BURGOS, N.; VAREJAO-SILVA, M. A.; COSTA, C. A. R. da. Zoneamento pedoclimático para a cultura do cajueiro (Anacardium occidentale L.) no Nordeste do Brasil e Norte de Minas Gerais. Fortaleza: Embrapa Agroindustrial Tropical; Recife: Embrapa-CNPS-ERP-NE, 2000. 30 p. (Embrapa Agroindústria Tropical. Boletim de Pesquisa, 27)| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 6. | | AGUIAR, M. de J. N; CAVALCANTI, A. C; BRAGA, C. C; BRITO, J. I. B. de; SILVA, M. A. V; BARROS, A. H. C; LUZ, L. R. Q. P. da; SILVA, F. B. R. e; COSTA, C. A. R. da; SILVA, D. F. da; PEREIRA, R. C. Zoneamento pedoclimatico para a cultura do cajueiro (Anacardium occidentale L.) no Estado da Bahia. Fortaleza: Embrapa Agroindustria Tropical, 2003. (Embrapa Agroindustria Tropical; Boletim de Pesquisa e Desenvolvimento, 13)| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 7. | | AGUIAR, M. de J. N.; CAVALCANTI, A. C.; BRAGA, C. C.; BRITO, J. I. B. de; SILVA, M. A. V.; BARROS, A. H. C.; LUZ, L. R. Q. P. da; SILVA, F. B. R. e; COSTA, C. A. R. da; SILVA, E. D. V.; SILVA, D. F. da; PEREIRA, R. C. Zoneamento pedoclimático para a cultura do cajueiro (Anacardium occidentale L.) no Estado da Bahia. Fortaleza: Embrapa Agroindústria Tropical, 2003. 32 p. (Embrapa Agroindústria Tropical. Boletim de pesquisa e desenvolvimento, 13).| Biblioteca(s): Embrapa Agroindústria Tropical; Embrapa Meio-Norte; Embrapa Unidades Centrais. |
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| 8. | | AGUIAR, M. de J. N.; BRAGA, C. C.; BRITO, J. I. B. de; SILVA, E. D. V.; SILVA, M. A. V.; BARROS, A. H. C.; CAVALCANTI, A. C.; NOGUEIRA, L. R. Q.; SILVA, F. B. R. e; COSTA, C. A. R. da. Zoneamento pedoclimatico para a cultura do cajueiro (Anacardium occidentale L.) no estado do Maranhao. Forataleza: Embrapa Agroindustria Tropical/Recife: Embrapa-CNPS-ERP-NE, 2000. 30p. (Embrapa Agroindustria Tropical. Boletim de Pesquisa, 38)| Biblioteca(s): Embrapa Meio-Norte. |
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| 9. | | AGUIAR, M. de J. N.; BRAGA, C. C.; BRITO, J. I. B. de; VAREJAO-SILVA, M. A.; BARROS, A. H. C.; CAVALCANTI, A. C.; NOGUEIRA, L. R. Q.; SILVA, F. B. R. e; COSTA, C. A. R. da; SILVA, D. F. da; SILVA, E. D. V.; PEREIRA, R. C. Zoneamento pedoclimático para a cultura do cajueiro (Anacardium occidentale L.) no Estado do Maranhão. Fortaleza: Embrapa Agroindustria Tropical,2000; Recife: Embrapa Solos-ERP-NE, 2000. 30 p. (Embrapa Agroindustria Tropical. Boletim de Pesquisa, 38). Acompanha mapa escala 1:1.000.000| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| Registros recuperados : 9 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
02/12/2010 |
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Data da última atualização: |
27/01/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
HERAI, R. H.; YAMAGISHI, M. E. B. |
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Afiliação: |
ROBERTO H. HERAI, IB/UNICAMP; MICHEL E. B. YAMAGISHI, CNPTIA. |
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Título: |
A bioinformatics approach to detect interchromosomal trans-splicing in bovine full length cDNA databanks. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: PLANT & ANIMAL GENOME CONFERENCE, 18., 2010, San Diego. Abstracts... [S.l.: s.n.], 2010. |
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Páginas: |
Não paginado. |
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Idioma: |
Inglês |
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Notas: |
P869. PAG-XVIII. |
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Conteúdo: |
Trans-splicing is an unusual form of RNA processing mechanism where distinct pre-mRNA sequences contribute to the formation of a single mRNA. It is common in nematodes and kinetoplastids, but it is rare in superior organisms. Nevertheless, some recent studies show that trans-splicing, although rare, may be more widespread than believed (Gingeras). Consequently, its identification in large databanks is very important for posteriori experimental confirmation. Recently, we have developed a Bioinformatic methodology that successfully found 16 inter-chromosomal trans-splicing candidate sequences in a large human full length cDNA (FlcDNA) databank (Herai & Yamagishi, in press). Unfortunately, our methodology was supposed to be applied on organisms with high quality reference genome, otherwise the number of false positive candidate sequences turned the analysis prohibitive. The problem is that many important model organisms have only draft assemblies. In order to overcome this problem, we extended our bioinformatics methodology applying additional filtering criteria and ad hoc algorithms necessary to deal with those cases. Using 83,048 bovine FlcDNA transcripts (NCBI, MGC, BGD) and Bos taurus UMD 3.0 genome assembly, our extended methodology successfully found 12 hybrid mRNAs which may be the first instances of bovine inter-chromosomal trans-splicing sequences, since the single reported bovine trans-splicing evidence was an intra-chromosomal instance (Roux et al.). Furthermore, just like in the human case, the bovine candidate sequence gene loci had many inverted repeat sequences which may support the conjecture that those sequences may be part of a non-spliceosome mediated trans-splicing mechanism (Di Segni et al.). MenosTrans-splicing is an unusual form of RNA processing mechanism where distinct pre-mRNA sequences contribute to the formation of a single mRNA. It is common in nematodes and kinetoplastids, but it is rare in superior organisms. Nevertheless, some recent studies show that trans-splicing, although rare, may be more widespread than believed (Gingeras). Consequently, its identification in large databanks is very important for posteriori experimental confirmation. Recently, we have developed a Bioinformatic methodology that successfully found 16 inter-chromosomal trans-splicing candidate sequences in a large human full length cDNA (FlcDNA) databank (Herai & Yamagishi, in press). Unfortunately, our methodology was supposed to be applied on organisms with high quality reference genome, otherwise the number of false positive candidate sequences turned the analysis prohibitive. The problem is that many important model organisms have only draft assemblies. In order to overcome this problem, we extended our bioinformatics methodology applying additional filtering criteria and ad hoc algorithms necessary to deal with those cases. Using 83,048 bovine FlcDNA transcripts (NCBI, MGC, BGD) and Bos taurus UMD 3.0 genome assembly, our extended methodology successfully found 12 hybrid mRNAs which may be the first instances of bovine inter-chromosomal trans-splicing sequences, since the single reported bovine trans-splicing evidence was an intra-chromosomal instance (Roux et al.). Furthermore, jus... Mostrar Tudo |
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Palavras-Chave: |
Algoritmo; Bioinformática. |
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Thesaurus NAL: |
Algorithms; Bioinformatics; RNA splicing. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/91866/1/869.pdf
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Marc: |
LEADER 02405nam a2200205 a 4500
001 1868482
005 2020-01-27
008 2010 bl uuuu u00u1 u #d
100 1 $aHERAI, R. H.
245 $aA bioinformatics approach to detect interchromosomal trans-splicing in bovine full length cDNA databanks.$h[electronic resource]
260 $aIn: PLANT & ANIMAL GENOME CONFERENCE, 18., 2010, San Diego. Abstracts... [S.l.: s.n.]$c2010
300 $aNão paginado.
500 $aP869. PAG-XVIII.
520 $aTrans-splicing is an unusual form of RNA processing mechanism where distinct pre-mRNA sequences contribute to the formation of a single mRNA. It is common in nematodes and kinetoplastids, but it is rare in superior organisms. Nevertheless, some recent studies show that trans-splicing, although rare, may be more widespread than believed (Gingeras). Consequently, its identification in large databanks is very important for posteriori experimental confirmation. Recently, we have developed a Bioinformatic methodology that successfully found 16 inter-chromosomal trans-splicing candidate sequences in a large human full length cDNA (FlcDNA) databank (Herai & Yamagishi, in press). Unfortunately, our methodology was supposed to be applied on organisms with high quality reference genome, otherwise the number of false positive candidate sequences turned the analysis prohibitive. The problem is that many important model organisms have only draft assemblies. In order to overcome this problem, we extended our bioinformatics methodology applying additional filtering criteria and ad hoc algorithms necessary to deal with those cases. Using 83,048 bovine FlcDNA transcripts (NCBI, MGC, BGD) and Bos taurus UMD 3.0 genome assembly, our extended methodology successfully found 12 hybrid mRNAs which may be the first instances of bovine inter-chromosomal trans-splicing sequences, since the single reported bovine trans-splicing evidence was an intra-chromosomal instance (Roux et al.). Furthermore, just like in the human case, the bovine candidate sequence gene loci had many inverted repeat sequences which may support the conjecture that those sequences may be part of a non-spliceosome mediated trans-splicing mechanism (Di Segni et al.).
650 $aAlgorithms
650 $aBioinformatics
650 $aRNA splicing
653 $aAlgoritmo
653 $aBioinformática
700 1 $aYAMAGISHI, M. E. B.
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Embrapa Agricultura Digital (CNPTIA) |
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