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| 1. |  | DUBS, F.; DECAËNS, T.; HEDDE, M.; GEOFFROY, J. J.; BOUTH, M. le; GARNIER, A.; BOGUCKI, C.; LANGLOIS, E.; BUREAU, F.; MARGERIE, P.; AUBERT, M.; LAVELLE, P. Surrounding landscape effects on local community of soil macrofauna detritivore. In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.| Biblioteca(s): Embrapa Florestas. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
12/12/2008 |
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Data da última atualização: |
24/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
PURCINO, A. A. C.; LIMA, T. R.; PINTO, A. C.; CARNEIRO, N. P.; SOUZA, I. R. P. de; MARRIEL, I. E.; PARENTONI, S. N.; DURAES, F.; CARVALHO, L. |
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Afiliação: |
ANTONIO ALVARO CORSETTI PURCINO, CNPMS; THALES LIMA ROCHA, CENARGEN; NEWTON PORTILHO CARNEIRO, CNPMS; ISABEL REGINA PRAZERES DE SOUZA, CNPMS; IVANILDO EVODIO MARRIEL, CNPMS; SIDNEY NETTO PARENTONI, CNPMS; Frederico Durães, Embrapa Milho e Sorgo; I. Carvalho, bolsista Embrapa Recursos Genéticos e Biotecnologia. |
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Título: |
Glutamine synthetase response to nitrate in maize genotypes of contrasting nitrogen use efficiency. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Maydica, Bergamo, v. 53, p. 101- 109, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
Although nitrogen use efficiency (NUE) is a complex trait. recent evidences have indicated that glutamine synthetase (GS, EC 6.3.1.2) is one of its major components. ln maize. GS is encoded by a multigene family and its gene products can be resolved into cytosolic (GSl) and chloroplastic (GS2) isoforms. Thercfore, the objective of this study was to determine possible systematie patterns of GS activity or isoenzyme expression response to nitrate nutrition among five maize (Zea mays 1.) SS inbred lines and five single cross hybrids, previously selected for contrasting NUE. Leaf GS biosynthetie activity significantly responded to nitrate nutrition at the 4-leaf seedling and at the 2-day after silking stages. but breeding for increased NUE did not lead to the development of genotypes with increased GS activity. After native polyacrylamide gcl electrophoreses (native-PAGE) and staining for transferase activity, GS produced distinct "up-per" and "lower" activity bands. ln leaves, these bands did not show a clear nitrate effect pattem, but in roots, the "upper" bands seemed to be more active in high nitrate plants, independently of the NUE of the genotypes tested. Protein gel analysis (SDS-PAGE), however. indicat-ed that breeding for increased NUE, under both low and high nitrate nutrition, led to the devclopment of inbred lines with more GSl than GS2 Cincreased GSl:GS2 ratio). Except for CMS 28-17.2 under low nitrate, this trend was not observed among the low NUE inbred lines. Altogether, these results suggest that breeding maize inbred !ines for better NUE, indirectly selected plants with an increased GSl content in rclation to GS2, without a significant effect on total GS biosynthetie activity. NUE in the singlc cross hybrids werc less influenccd by the GSl:GS2 ratio, but one possiblc explanation for this discrepancy may have becn ascribcd to the less imense sc!ection pressure for increased NUE undergone by the hybrids. MenosAlthough nitrogen use efficiency (NUE) is a complex trait. recent evidences have indicated that glutamine synthetase (GS, EC 6.3.1.2) is one of its major components. ln maize. GS is encoded by a multigene family and its gene products can be resolved into cytosolic (GSl) and chloroplastic (GS2) isoforms. Thercfore, the objective of this study was to determine possible systematie patterns of GS activity or isoenzyme expression response to nitrate nutrition among five maize (Zea mays 1.) SS inbred lines and five single cross hybrids, previously selected for contrasting NUE. Leaf GS biosynthetie activity significantly responded to nitrate nutrition at the 4-leaf seedling and at the 2-day after silking stages. but breeding for increased NUE did not lead to the development of genotypes with increased GS activity. After native polyacrylamide gcl electrophoreses (native-PAGE) and staining for transferase activity, GS produced distinct "up-per" and "lower" activity bands. ln leaves, these bands did not show a clear nitrate effect pattem, but in roots, the "upper" bands seemed to be more active in high nitrate plants, independently of the NUE of the genotypes tested. Protein gel analysis (SDS-PAGE), however. indicat-ed that breeding for increased NUE, under both low and high nitrate nutrition, led to the devclopment of inbred lines with more GSl than GS2 Cincreased GSl:GS2 ratio). Except for CMS 28-17.2 under low nitrate, this trend was not observed among the low NUE inbred lines. Alt... Mostrar Tudo |
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Palavras-Chave: |
Glutamine synthetase; Nitrogcn use effi-cicncy; Nitrogen use efficiency; Zea mays L. |
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Thesagro: |
Milho; Zea Mays. |
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Categoria do assunto: |
--
S Ciências Biológicas |
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Marc: |
LEADER 02778naa a2200289 a 4500
001 1491623
005 2018-05-24
008 2008 bl uuuu u00u1 u #d
100 1 $aPURCINO, A. A. C.
245 $aGlutamine synthetase response to nitrate in maize genotypes of contrasting nitrogen use efficiency.$h[electronic resource]
260 $c2008
520 $aAlthough nitrogen use efficiency (NUE) is a complex trait. recent evidences have indicated that glutamine synthetase (GS, EC 6.3.1.2) is one of its major components. ln maize. GS is encoded by a multigene family and its gene products can be resolved into cytosolic (GSl) and chloroplastic (GS2) isoforms. Thercfore, the objective of this study was to determine possible systematie patterns of GS activity or isoenzyme expression response to nitrate nutrition among five maize (Zea mays 1.) SS inbred lines and five single cross hybrids, previously selected for contrasting NUE. Leaf GS biosynthetie activity significantly responded to nitrate nutrition at the 4-leaf seedling and at the 2-day after silking stages. but breeding for increased NUE did not lead to the development of genotypes with increased GS activity. After native polyacrylamide gcl electrophoreses (native-PAGE) and staining for transferase activity, GS produced distinct "up-per" and "lower" activity bands. ln leaves, these bands did not show a clear nitrate effect pattem, but in roots, the "upper" bands seemed to be more active in high nitrate plants, independently of the NUE of the genotypes tested. Protein gel analysis (SDS-PAGE), however. indicat-ed that breeding for increased NUE, under both low and high nitrate nutrition, led to the devclopment of inbred lines with more GSl than GS2 Cincreased GSl:GS2 ratio). Except for CMS 28-17.2 under low nitrate, this trend was not observed among the low NUE inbred lines. Altogether, these results suggest that breeding maize inbred !ines for better NUE, indirectly selected plants with an increased GSl content in rclation to GS2, without a significant effect on total GS biosynthetie activity. NUE in the singlc cross hybrids werc less influenccd by the GSl:GS2 ratio, but one possiblc explanation for this discrepancy may have becn ascribcd to the less imense sc!ection pressure for increased NUE undergone by the hybrids.
650 $aMilho
650 $aZea Mays
653 $aGlutamine synthetase
653 $aNitrogcn use effi-cicncy
653 $aNitrogen use efficiency
653 $aZea mays L
700 1 $aLIMA, T. R.
700 1 $aPINTO, A. C.
700 1 $aCARNEIRO, N. P.
700 1 $aSOUZA, I. R. P. de
700 1 $aMARRIEL, I. E.
700 1 $aPARENTONI, S. N.
700 1 $aDURAES, F.
700 1 $aCARVALHO, L.
773 $tMaydica, Bergamo$gv. 53, p. 101- 109, 2008.
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