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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Norte / UEP-Parnaíba; Embrapa Meio-Norte. |
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Data corrente: |
30/06/1997 |
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Data da última atualização: |
20/11/2009 |
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Autoria: |
KHAN, R. I.; GATEHOUSE, J. A.; BOULTER, D. |
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Afiliação: |
Department of Botany, University of Durham. |
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Título: |
The seed proteins of cowpea (Vigna unguiculata L. Walp.). |
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Ano de publicação: |
1980 |
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Fonte/Imprenta: |
Journal of Experimental Botany, v. 31, n. 125, p.1599-1611, 1980. |
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Idioma: |
Inglês |
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Conteúdo: |
Cowpea (Vigna unguiculata L. Walp.) seeds were shown to contain a major globulin protein of mol. wt. l70 000 which was heterogenous; two components could be separated by gel electrophoresis at pH 4.0, one containing subunits of mol. wt. 52 000 and 58 000, the former predominating, the other containing subunits of mol. wt. 52 000, 58 000, and 63 000 in roughly equal amounts. The 58 000 and 63 000 mol, wt. subunits contained covalently bound carbohydrate. All three subunit types were shown to contain extensive sequence homology by tryptic mapping; cleavage with cyanogen bromide also indicated homology between subunit types. The 52 000 and 58 000 mol. wt. subunits were shown to be heterogeneous within each size class. The major globulin protein ispossibly a trimer, three subunits from the various types of 52 000, 58 000, and 53 000 mol. wt. subunits assembling to form a molecule. Of the other proteins identified in cowpea seeds, one was a globulin protein of mol. wt. 300.400 000 containing disulphide-linked paris of subunits of mol. wt. 62 000 and 18 500. Another globulin protein disulphide-linked dimers of a 56 000 mol. wt subunit. A major albumin protein was a monomer, subunit mol. wt. 105 000; other albumin proteins present in significant amount (Subunit mol. wts 32 000, 22 500, and lower) had lower mol. wts. |
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Palavras-Chave: |
Albuminas; Caupi; Cowpea; Globulinas; Protein. |
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Thesagro: |
Feijão; Proteína; Vigna Unguiculata. |
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Thesaurus Nal: |
albumins; globulins; proteins. |
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Categoria do assunto: |
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Marc: |
LEADER 02020naa a2200277 a 4500
001 1067075
005 2009-11-20
008 1980 bl --- 0-- u #d
100 1 $aKHAN, R. I.
245 $aThe seed proteins of cowpea (Vigna unguiculata L. Walp.).
260 $c1980
520 $aCowpea (Vigna unguiculata L. Walp.) seeds were shown to contain a major globulin protein of mol. wt. l70 000 which was heterogenous; two components could be separated by gel electrophoresis at pH 4.0, one containing subunits of mol. wt. 52 000 and 58 000, the former predominating, the other containing subunits of mol. wt. 52 000, 58 000, and 63 000 in roughly equal amounts. The 58 000 and 63 000 mol, wt. subunits contained covalently bound carbohydrate. All three subunit types were shown to contain extensive sequence homology by tryptic mapping; cleavage with cyanogen bromide also indicated homology between subunit types. The 52 000 and 58 000 mol. wt. subunits were shown to be heterogeneous within each size class. The major globulin protein ispossibly a trimer, three subunits from the various types of 52 000, 58 000, and 53 000 mol. wt. subunits assembling to form a molecule. Of the other proteins identified in cowpea seeds, one was a globulin protein of mol. wt. 300.400 000 containing disulphide-linked paris of subunits of mol. wt. 62 000 and 18 500. Another globulin protein disulphide-linked dimers of a 56 000 mol. wt subunit. A major albumin protein was a monomer, subunit mol. wt. 105 000; other albumin proteins present in significant amount (Subunit mol. wts 32 000, 22 500, and lower) had lower mol. wts.
650 $aalbumins
650 $aglobulins
650 $aproteins
650 $aFeijão
650 $aProteína
650 $aVigna Unguiculata
653 $aAlbuminas
653 $aCaupi
653 $aCowpea
653 $aGlobulinas
653 $aProtein
700 1 $aGATEHOUSE, J. A.
700 1 $aBOULTER, D.
773 $tJournal of Experimental Botany$gv. 31, n. 125, p.1599-1611, 1980.
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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