| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Hortaliças. |
|
Data corrente: |
10/05/1995 |
|
Data da última atualização: |
10/05/1995 |
|
Autoria: |
TELES, F. F. F.; SALES FILHO, J. B. de; GIUDICE, M. de L. P. del; BORGES, V. E. de L. e. |
|
Afiliação: |
UFV - 36570-000 Vicosa, MG. |
|
Título: |
Carboidratos digeriveis de algumas tuberosas comercializadas em Vicosa, Minas Gerais. |
|
Ano de publicação: |
1986 |
|
Fonte/Imprenta: |
Revista Ceres, Vicosa, v.33, n.186, p.189-193, 1986. |
|
ISSN: |
0034-737X |
|
Idioma: |
Português |
|
Palavras-Chave: |
Beet; Carrot; Mandioquinha-salsa; Peruvian carrot; Potato; Sweet potato. |
|
Thesagro: |
Arracacia Xanthorrhiza; Batata; Batata Doce; Beta Vulgaris; Beterraba; Cenoura; Colocasia Esculenta; Daucus Carota; Ipomoea Batatas; Solanum Tuberosum; Taro. |
|
Thesaurus Nal: |
carbohydrates; tubers. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01029naa a2200385 a 4500
001 1752993
005 1995-05-10
008 1986 bl uuuu u00u1 u #d
022 $a0034-737X
100 1 $aTELES, F. F. F.
245 $aCarboidratos digeriveis de algumas tuberosas comercializadas em Vicosa, Minas Gerais.
260 $c1986
650 $acarbohydrates
650 $atubers
650 $aArracacia Xanthorrhiza
650 $aBatata
650 $aBatata Doce
650 $aBeta Vulgaris
650 $aBeterraba
650 $aCenoura
650 $aColocasia Esculenta
650 $aDaucus Carota
650 $aIpomoea Batatas
650 $aSolanum Tuberosum
650 $aTaro
653 $aBeet
653 $aCarrot
653 $aMandioquinha-salsa
653 $aPeruvian carrot
653 $aPotato
653 $aSweet potato
700 1 $aSALES FILHO, J. B. de
700 1 $aGIUDICE, M. de L. P. del
700 1 $aBORGES, V. E. de L. e.
773 $tRevista Ceres, Vicosa$gv.33, n.186, p.189-193, 1986.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Hortaliças (CNPH) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
19/09/2019 |
|
Data da última atualização: |
06/12/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
FERREIRA, C. F.; GUTIERREZ, D. L.; KREUZE, J. F.; ISKRA-CARUANA, M. L.; CHABANNES, M.; BARBOSA, A. C. O.; SANTOS, T. A.; SILVA, A. G. S.; SANTOS, R. M. F.; AMORIM, E. P.; OLIVEIRA, S. A. S. de; JESUS, O. N. de. |
|
Afiliação: |
CLAUDIA FORTES FERREIRA, CNPMF; D. L. GUTIERREZ, INIA; J. F. KREUZE, CIP; M. L. ISKRA-CARUANA, CIRAD; M. CHABANNES, CIRAD; A. C. O. BARBOSA, UFRB; T. A. SANTOS, UFRB; A. G. S. SILVA, UFRB; R.M.F. SANTOS, UEFS; EDSON PERITO AMORIM, CNPMF; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; ONILDO NUNES DE JESUS, CNPMF. |
|
Título: |
Rapid plant DNA and RNA extraction protocol using a bench drill Brief Note. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Genetics and Molecular Research, v. 18, n.3, 2019. |
|
Idioma: |
Inglês |
|
Notas: |
gmr18394 |
|
Conteúdo: |
Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint. |
|
Thesagro: |
Melhoramento Vegetal. |
|
Thesaurus NAL: |
Breeding and Genetic Improvement. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/202165/1/-rapid-plant-dna-and-rna-extraction-protocol-using-bench-drill.pdf
|
|
Marc: |
LEADER 02135naa a2200289 a 4500
001 2112332
005 2019-12-06
008 2019 bl uuuu u00u1 u #d
100 1 $aFERREIRA, C. F.
245 $aRapid plant DNA and RNA extraction protocol using a bench drill Brief Note.$h[electronic resource]
260 $c2019
500 $agmr18394
520 $aPlant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint.
650 $aBreeding and Genetic Improvement
650 $aMelhoramento Vegetal
700 1 $aGUTIERREZ, D. L.
700 1 $aKREUZE, J. F.
700 1 $aISKRA-CARUANA, M. L.
700 1 $aCHABANNES, M.
700 1 $aBARBOSA, A. C. O.
700 1 $aSANTOS, T. A.
700 1 $aSILVA, A. G. S.
700 1 $aSANTOS, R. M. F.
700 1 $aAMORIM, E. P.
700 1 $aOLIVEIRA, S. A. S. de
700 1 $aJESUS, O. N. de
773 $tGenetics and Molecular Research$gv. 18, n.3, 2019.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
