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Registro Completo |
Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
21/02/2019 |
Data da última atualização: |
13/01/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, C. G. da; MARTINS, C. F.; CUMPA, H. C. B.; FONSECA NETO, A. M. da; CARDOSO, T. C.; FRANCO, M. M.; MENDONÇA, A. dos S.; LEME, L. de O.; BORGES, J. R. J.; MALAQUIAS, J. V.; BAO, S. N. |
Afiliação: |
CAROLINA GONZALES DA SILVA, UNB; CARLOS FREDERICO MARTINS, CPAC; HEIDI CHRISTINA BESSLER CUMPA, CPAC; ALVARO MORAES DA FONSECA NETO, CPAC; TEREZA CRISTINA CARDOSO, UNESP; MAURICIO MACHAIM FRANCO, Cenargen; ANELISE DOS SANTOS MENDONÇA, UNIVERSIDADE DE UBERLÂNDIA; LIGIANE DE OLIVEIRA LEME, Cenargen; JOSÉ RENATO JUNQUEIRA BORGES, UNB; JUACI VITORIA MALAQUIAS, CPAC; SONIA NAIR BAO, UNB. |
Título: |
Use of trichostatin A alters the expression of HDAC3 and KAT2 and improves in vitro development of bovine embryos cloned using less methylated mesenchymal stem cells. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Reproduction in Domestic Animals, v. 54, n. 2, p. 289-299, February 2019. |
DOI: |
10.1111/rda.13360 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The aim of this work was to investigate the methylation and hydroxymethylation status of mesenchymal stem cells (MSC) from amniotic fluid (MSC-AF), adipose tissue (MSC-AT) and fibroblasts (FIB-control) and to verify the effect of trichostatin A (TSA) on gene expression and development of cloned bovine embryos produced using these cells. Characterization of MSC from two animals (BOV1 and BOV2) was performed by flow cytometry, immunophenotyping and analysis of cellular differentiation genes expression. The cells were used in the nuclear transfer in the absence or presence of 50 nM TSA for 20 hr in embryo culture. Expression of HDAC1, HDAC3 and KAT2A genes was measured in embryos by qRT-PCR. Methylation results showed difference between animals, with MSC from BOV2 demonstrating lower methylation rate than BOV1. Meanwhile, MSC-AF were less hydroxymethylated for both animals. MSC-AF from BOV2 produced 44.92 ± 8.88% of blastocysts when embryos were exposed to TSA and similar to embryo rate of MSC-AT also treated with TSA (37.96 ± 15.80%). However, when methylation was lower in FIB compared to MSC, as found in BOV1, the use of TSA was not sufficient to increase embryo production. MSC-AF embryos expressed less HDAC3 when treated with TSA, and expression of KAT2A was higher in embryos produced with all MSC and treated with TSA than embryos produced with FIB. The use of MSC less methylated and more hydroxymethylated in combination with embryo incubation with TSA can induce lower expression of HDAC3 and higher expression of KAT2A in the embryos and consequently improve bovine embryo production. MenosAbstract: The aim of this work was to investigate the methylation and hydroxymethylation status of mesenchymal stem cells (MSC) from amniotic fluid (MSC-AF), adipose tissue (MSC-AT) and fibroblasts (FIB-control) and to verify the effect of trichostatin A (TSA) on gene expression and development of cloned bovine embryos produced using these cells. Characterization of MSC from two animals (BOV1 and BOV2) was performed by flow cytometry, immunophenotyping and analysis of cellular differentiation genes expression. The cells were used in the nuclear transfer in the absence or presence of 50 nM TSA for 20 hr in embryo culture. Expression of HDAC1, HDAC3 and KAT2A genes was measured in embryos by qRT-PCR. Methylation results showed difference between animals, with MSC from BOV2 demonstrating lower methylation rate than BOV1. Meanwhile, MSC-AF were less hydroxymethylated for both animals. MSC-AF from BOV2 produced 44.92 ± 8.88% of blastocysts when embryos were exposed to TSA and similar to embryo rate of MSC-AT also treated with TSA (37.96 ± 15.80%). However, when methylation was lower in FIB compared to MSC, as found in BOV1, the use of TSA was not sufficient to increase embryo production. MSC-AF embryos expressed less HDAC3 when treated with TSA, and expression of KAT2A was higher in embryos produced with all MSC and treated with TSA than embryos produced with FIB. The use of MSC less methylated and more hydroxymethylated in combination with embryo incubation with TSA can induce l... Mostrar Tudo |
Palavras-Chave: |
Acetilação de histona; Célula adiposa; Célula amniótica. |
Thesagro: |
Bovino; Clonagem; DNA; Melhoramento Genético Animal; Metilação. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02722naa a2200349 a 4500 001 2106339 005 2020-01-13 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1111/rda.13360$2DOI 100 1 $aSILVA, C. G. da 245 $aUse of trichostatin A alters the expression of HDAC3 and KAT2 and improves in vitro development of bovine embryos cloned using less methylated mesenchymal stem cells.$h[electronic resource] 260 $c2019 520 $aAbstract: The aim of this work was to investigate the methylation and hydroxymethylation status of mesenchymal stem cells (MSC) from amniotic fluid (MSC-AF), adipose tissue (MSC-AT) and fibroblasts (FIB-control) and to verify the effect of trichostatin A (TSA) on gene expression and development of cloned bovine embryos produced using these cells. Characterization of MSC from two animals (BOV1 and BOV2) was performed by flow cytometry, immunophenotyping and analysis of cellular differentiation genes expression. The cells were used in the nuclear transfer in the absence or presence of 50 nM TSA for 20 hr in embryo culture. Expression of HDAC1, HDAC3 and KAT2A genes was measured in embryos by qRT-PCR. Methylation results showed difference between animals, with MSC from BOV2 demonstrating lower methylation rate than BOV1. Meanwhile, MSC-AF were less hydroxymethylated for both animals. MSC-AF from BOV2 produced 44.92 ± 8.88% of blastocysts when embryos were exposed to TSA and similar to embryo rate of MSC-AT also treated with TSA (37.96 ± 15.80%). However, when methylation was lower in FIB compared to MSC, as found in BOV1, the use of TSA was not sufficient to increase embryo production. MSC-AF embryos expressed less HDAC3 when treated with TSA, and expression of KAT2A was higher in embryos produced with all MSC and treated with TSA than embryos produced with FIB. The use of MSC less methylated and more hydroxymethylated in combination with embryo incubation with TSA can induce lower expression of HDAC3 and higher expression of KAT2A in the embryos and consequently improve bovine embryo production. 650 $aBovino 650 $aClonagem 650 $aDNA 650 $aMelhoramento Genético Animal 650 $aMetilação 653 $aAcetilação de histona 653 $aCélula adiposa 653 $aCélula amniótica 700 1 $aMARTINS, C. F. 700 1 $aCUMPA, H. C. B. 700 1 $aFONSECA NETO, A. M. da 700 1 $aCARDOSO, T. C. 700 1 $aFRANCO, M. M. 700 1 $aMENDONÇA, A. dos S. 700 1 $aLEME, L. de O. 700 1 $aBORGES, J. R. J. 700 1 $aMALAQUIAS, J. V. 700 1 $aBAO, S. N. 773 $tReproduction in Domestic Animals$gv. 54, n. 2, p. 289-299, February 2019.
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Registro Completo
Biblioteca(s): |
Embrapa Solos. |
Data corrente: |
27/03/2009 |
Data da última atualização: |
27/03/2009 |
Tipo da produção científica: |
Monitoramento/Zoneamento |
Autoria: |
ACCIOLY, L. J. de O.; SILVA, A. B. da; SILVEIRA, H. L. F. da; LOPES, H. L.; FONSECA, J. C. da; MENEZES, A. A. de A.; SILVA, J. A. da; SILVA, E. A. da; FRANÇA, A. E. D. de; SILVA, F. F. da; GUIMARÃES, L. M.; AZEVEDO, V. W. B.; SILVA, E. A. da. |
Afiliação: |
Luciano José de Oliveira Accioly, Embrapa Solos; Ademar Barros da Silva, Embrapa Solos; Hilton Luís Ferraz da Silveira, Embrapa Solos; Hélio Leandro Lopes, Embrapa Solos; João Cordeiro da Fonseca, Embrapa Solos; Arnaldo A. de Alecrim Menezes, Embrapa Solos; Juliana Alves da Silva, Embrapa Solos; Eduardo Alves da Silva, Embrapa Solos; Álvaro Eugênio Duarte de França, Embrapa Solos; Fábio Fernandes da Silva, Embrapa Solos; Luciano Macedo Guimarães, Embrapa Solos; Verônica Wilma Bezerra Azevedo, Embrapa Solos; Eudmar Alves da Silva, Embrapa Solos. |
Título: |
Mapa relatório de uso e cobertura das terras do estado de Alagoas - Folha Piranhas. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Recife: Embrapa Solos UEP, 2008. |
Páginas: |
1 mapa |
Descrição Física: |
mapa color.; escala 1:100.000. |
Idioma: |
Português |
Categoria do assunto: |
-- |
Marc: |
LEADER 00766nem a2200253 a 4500 001 1340054 005 2009-03-27 008 2008 bl uuuu u0uu1 u #d 100 1 $aACCIOLY, L. J. de O. 245 $aMapa relatório de uso e cobertura das terras do estado de Alagoas - Folha Piranhas. 260 $aRecife: Embrapa Solos UEP$c2008 300 $a1 mapa$cmapa color.; escala 1:100.000. 700 1 $aSILVA, A. B. da 700 1 $aSILVEIRA, H. L. F. da 700 1 $aLOPES, H. L. 700 1 $aFONSECA, J. C. da 700 1 $aMENEZES, A. A. de A. 700 1 $aSILVA, J. A. da 700 1 $aSILVA, E. A. da 700 1 $aFRANÇA, A. E. D. de 700 1 $aSILVA, F. F. da 700 1 $aGUIMARÃES, L. M. 700 1 $aAZEVEDO, V. W. B. 700 1 $aSILVA, E. A. da
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