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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
15/02/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
SANTOS, M. L. T.; BORGES, A. A.; QUEIROZ NETA, L. B.; SANTOS, M. V. O.; OLIVEIRA, M. F.; SILVA, A. R.; PEREIRA, A. F. |
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Afiliação: |
MAGDA L. T. SANTOS, UFERSA; ALANA A. BORGES, UFERSA; LUIZA B. QUEIROZ NETA, UFERSA; MARIA V. O. SANTOS, UFERSA; MOACIR F. OLIVEIRA, UFERSA; ALEXANDRE R. SILVA, UFERSA; ALEXANDRA F. PEREIRA, UFERSA. |
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Título: |
In vitro culture of somatic cells derived from ear tissue of collared peccary (Pecari tajacu Linnaeus, 1758) in medium with different requirements. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 12, p. 1194-1202, dez. 2016. |
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Idioma: |
Português |
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Conteúdo: |
The maintenance of metabolic activities during the in vitro culture of somatic cells of wild animals, especially collared peccary (Pecari tajacu), is an interesting step in conservation of these cells for the use in nuclear transfer. In this context, it is necessary to optimize the culture conditions of somatic cells by the establishment of appropriate supplementation to the media. Therefore, this study aimed to analyze the composition of the culture means of somatic cell derived from ear tissue of collared peccaries, evaluating concentrations of fetal bovine serum (FBS; 10% vs. 20%) and epidermal growth factor (EGF; 5ng/mL vs. 10ng/mL). Tissues were submitted to primary culture and subcultures for 40 days and cells were analyzed for morphology, adhesion, subconfluence, and proliferative activity to develop the growth curve and to determine the population doubling time (PDT), viability, and functional/metabolic activity. No difference was observed between the concentrations of FBS for several parameters, except for viability [FBS10: 85.6% vs. FBS20: 98.2%], PDT [FBS10: 155.4h vs. 77.2h], and functional/metabolic assay [FBS10: 0.57?0.55 vs. FBS20: 0.82?0.99 (D5-D7)]. For the EGF in culture, no difference was observed in the evaluated parameters. In all experiments, the growth curves were typical S-shape and the cells passed through a lag, logarithmic, and plateau phase. In conclusion, 20% FBS is suitable for the recovery of somatic cells; nevertheless, EGF does not improve the quality of growing these cells. To our knowledge, this is the first study culturing somatic cells of collared peccaries. MenosThe maintenance of metabolic activities during the in vitro culture of somatic cells of wild animals, especially collared peccary (Pecari tajacu), is an interesting step in conservation of these cells for the use in nuclear transfer. In this context, it is necessary to optimize the culture conditions of somatic cells by the establishment of appropriate supplementation to the media. Therefore, this study aimed to analyze the composition of the culture means of somatic cell derived from ear tissue of collared peccaries, evaluating concentrations of fetal bovine serum (FBS; 10% vs. 20%) and epidermal growth factor (EGF; 5ng/mL vs. 10ng/mL). Tissues were submitted to primary culture and subcultures for 40 days and cells were analyzed for morphology, adhesion, subconfluence, and proliferative activity to develop the growth curve and to determine the population doubling time (PDT), viability, and functional/metabolic activity. No difference was observed between the concentrations of FBS for several parameters, except for viability [FBS10: 85.6% vs. FBS20: 98.2%], PDT [FBS10: 155.4h vs. 77.2h], and functional/metabolic assay [FBS10: 0.57?0.55 vs. FBS20: 0.82?0.99 (D5-D7)]. For the EGF in culture, no difference was observed in the evaluated parameters. In all experiments, the growth curves were typical S-shape and the cells passed through a lag, logarithmic, and plateau phase. In conclusion, 20% FBS is suitable for the recovery of somatic cells; nevertheless, EGF does not improve th... Mostrar Tudo |
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Palavras-Chave: |
Mitotic factor; Somatic tissue. |
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Thesagro: |
Tayassu Tajacu. |
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Thesaurus Nal: |
Culture media; Protein sources; Somatic cells; Wild animals. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/155804/1/In-vitro-culutre-of-somatic.pdf
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Marc: |
LEADER 02511naa a2200277 a 4500
001 2064367
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aSANTOS, M. L. T.
245 $aIn vitro culture of somatic cells derived from ear tissue of collared peccary (Pecari tajacu Linnaeus, 1758) in medium with different requirements.$h[electronic resource]
260 $c2016
520 $aThe maintenance of metabolic activities during the in vitro culture of somatic cells of wild animals, especially collared peccary (Pecari tajacu), is an interesting step in conservation of these cells for the use in nuclear transfer. In this context, it is necessary to optimize the culture conditions of somatic cells by the establishment of appropriate supplementation to the media. Therefore, this study aimed to analyze the composition of the culture means of somatic cell derived from ear tissue of collared peccaries, evaluating concentrations of fetal bovine serum (FBS; 10% vs. 20%) and epidermal growth factor (EGF; 5ng/mL vs. 10ng/mL). Tissues were submitted to primary culture and subcultures for 40 days and cells were analyzed for morphology, adhesion, subconfluence, and proliferative activity to develop the growth curve and to determine the population doubling time (PDT), viability, and functional/metabolic activity. No difference was observed between the concentrations of FBS for several parameters, except for viability [FBS10: 85.6% vs. FBS20: 98.2%], PDT [FBS10: 155.4h vs. 77.2h], and functional/metabolic assay [FBS10: 0.57?0.55 vs. FBS20: 0.82?0.99 (D5-D7)]. For the EGF in culture, no difference was observed in the evaluated parameters. In all experiments, the growth curves were typical S-shape and the cells passed through a lag, logarithmic, and plateau phase. In conclusion, 20% FBS is suitable for the recovery of somatic cells; nevertheless, EGF does not improve the quality of growing these cells. To our knowledge, this is the first study culturing somatic cells of collared peccaries.
650 $aCulture media
650 $aProtein sources
650 $aSomatic cells
650 $aWild animals
650 $aTayassu Tajacu
653 $aMitotic factor
653 $aSomatic tissue
700 1 $aBORGES, A. A.
700 1 $aQUEIROZ NETA, L. B.
700 1 $aSANTOS, M. V. O.
700 1 $aOLIVEIRA, M. F.
700 1 $aSILVA, A. R.
700 1 $aPEREIRA, A. F.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 12, p. 1194-1202, dez. 2016.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
10/11/2022 |
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Data da última atualização: |
12/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MARQUES-BASTOS, S. L. S.; COELHO, M. L. V.; SANTOS, I. N. de S.; MORENO, D. S. A.; BARRIAS, E. S.; MENDONÇA, J. F. M. de; MENDONCA, L. C.; LANGE, C. C.; BRITO, M. A. V. de P.; BASTOS, M. do C. de F. |
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Afiliação: |
SELDA LOASE SALUSTIANO MARQUES-BASTOS, Universidade Federal do Rio de Janeiro; MARCUS LÍVIO VARELLA COELHO, Universidade Federal do Rio de Janeiro; ILANA NASCIMENTO DE SOUSA SANTOS, Universidade Federal do Rio de Janeiro; DANIELA SALES ALVIANO MORENO, Universidade Federal do Rio de Janeiro; EMILE SANTOS BARRIAS, Instituto Nacional de Metrologia, Qualidade E Tecnologia; JULIANA FRANÇA MONTEIRO DE MENDONÇA; LETICIA CALDAS MENDONCA, CNPGL; CARLA CHRISTINE LANGE, CNPGL; MARIA APARECIDA VASCONCELOS DE PAIVA BRITO; MARIA DO CARMO DE FREIRE BASTOS, Universidade Federal do Rio de Janeiro. |
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Título: |
Effects of the natural antimicrobial peptide aureocin A53 on cells of Staphylococcus aureus and Streptococcus agalactiae involved in bovine mastitis in the excised teat model. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
World Journal of Microbiology & Biotechnology, v. 39, n. 1, article 5, 2023. |
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DOI: |
https://doi.org/10.1007/s11274-022-03443-w |
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Idioma: |
Inglês |
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Conteúdo: |
Aureocin A53 is an N-formylated antimicrobial peptide (AMP) produced by Staphylococcus aureus. Aureocin A53 has a broad spectrum of antimicrobial activity against human and animal pathogens. In the present study, its antagonistic activity was investigated towards 30 strains of S. aureus and 30 strains of Streptococcus spp. isolated from bovine mastitis cases in Brazil. Bovine mastitis is a disease that causes a major economic impact worldwide. Aureocin A53 inhibited the growth of all 60 strains tested, including multidrug-resistant streptococcal isolates and strains of S. aureus belonging to different pulsotypes. This AMP proved to be bactericidal against the six target strains randomly selected among staphylococci and streptococci, also exhibiting a lytic mode of action against the staphylococcal cells. Furthermore, it was determined that 2,048 AU/mL of the AMP were required to inhibit 99.99% of the cell growth of the strain less sensitive to aureocin A53. Aureocin A53 was not toxic to bovine mammary gland epithelial cells after a 24-h exposure and maintained its antimicrobial activity when tested in the excised-teat model against strains of S. aureus and Streptococcus agalactiae, the species responsible for most intramammary infections, not only in Brazil but in other countries as well. Therefore, the use of aureocin A53 in the development of new pharmacological products for the prophylaxis and/or treatment of bovine mastitis was considered promising. |
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Palavras-Chave: |
Aureocina A53; Mastite; Peptídeo antimicrobiano. |
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Thesagro: |
Bovino; Estreptococo. |
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Thesaurus NAL: |
Antimicrobial peptides; Bovine mastitis; Staphylococcus aureus subsp. aureus; Streptococcus. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02660naa a2200349 a 4500
001 2148198
005 2022-12-12
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11274-022-03443-w$2DOI
100 1 $aMARQUES-BASTOS, S. L. S.
245 $aEffects of the natural antimicrobial peptide aureocin A53 on cells of Staphylococcus aureus and Streptococcus agalactiae involved in bovine mastitis in the excised teat model.$h[electronic resource]
260 $c2023
520 $aAureocin A53 is an N-formylated antimicrobial peptide (AMP) produced by Staphylococcus aureus. Aureocin A53 has a broad spectrum of antimicrobial activity against human and animal pathogens. In the present study, its antagonistic activity was investigated towards 30 strains of S. aureus and 30 strains of Streptococcus spp. isolated from bovine mastitis cases in Brazil. Bovine mastitis is a disease that causes a major economic impact worldwide. Aureocin A53 inhibited the growth of all 60 strains tested, including multidrug-resistant streptococcal isolates and strains of S. aureus belonging to different pulsotypes. This AMP proved to be bactericidal against the six target strains randomly selected among staphylococci and streptococci, also exhibiting a lytic mode of action against the staphylococcal cells. Furthermore, it was determined that 2,048 AU/mL of the AMP were required to inhibit 99.99% of the cell growth of the strain less sensitive to aureocin A53. Aureocin A53 was not toxic to bovine mammary gland epithelial cells after a 24-h exposure and maintained its antimicrobial activity when tested in the excised-teat model against strains of S. aureus and Streptococcus agalactiae, the species responsible for most intramammary infections, not only in Brazil but in other countries as well. Therefore, the use of aureocin A53 in the development of new pharmacological products for the prophylaxis and/or treatment of bovine mastitis was considered promising.
650 $aAntimicrobial peptides
650 $aBovine mastitis
650 $aStaphylococcus aureus subsp. aureus
650 $aStreptococcus
650 $aBovino
650 $aEstreptococo
653 $aAureocina A53
653 $aMastite
653 $aPeptídeo antimicrobiano
700 1 $aCOELHO, M. L. V.
700 1 $aSANTOS, I. N. de S.
700 1 $aMORENO, D. S. A.
700 1 $aBARRIAS, E. S.
700 1 $aMENDONÇA, J. F. M. de
700 1 $aMENDONCA, L. C.
700 1 $aLANGE, C. C.
700 1 $aBRITO, M. A. V. de P.
700 1 $aBASTOS, M. do C. de F.
773 $tWorld Journal of Microbiology & Biotechnology$gv. 39, n. 1, article 5, 2023.
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